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A cDNA microarray technique applied for analysis of global gene expression profiles in tributyltin-exposed ascidians 总被引:2,自引:0,他引:2
To analyze global gene expressions, we constructed a cDNA microarray from a basal chordate, the ascidian Ciona intestinalis. Ciona is a cosmopolitan species and a genomic analysis of Ciona revealed that ascidians had approximately 15,500 protein-coding genes. Our "Ciona intestinalis cDNA chip version 1 (Ci cDNA chip ver. 1)" has arrayed 13,400 unique Ciona cDNAs. To establish a detection system for gene expression profiles in wild ascidians using a cDNA microarray, we analyzed gene expressions in the whole body of Ciona adults after exposure to 100 nM tributyltin (TBT) for 24 h. In our preliminary array data using Ci cDNA chip ver. 1, we found more than 200 genes that showed strong differential expressions. These genes encoded proteins that were concerned with stress response, detoxification, oxidoreduction reaction, biosynthesis, and catabolism. This, the first large cDNA microarray of this animal, should facilitate analyses of global gene expressions following exposure to TBT. 相似文献
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Michinobu Kuwae Azumi Yamashita Yuichi Hayami Atsushi Kaneda Takashige Sugimoto Yoshio Inouchi Atsuko Amano Hidetaka Takeoka 《Journal of Oceanography》2006,62(5):657-666
In order to examine the responses of primary productivity in the southern coastal sea of Japan to the Pacific Decadal Oscillation
(PDO) in the 20th century, sedimentary records of diatom productivity (diatom valve fluxes) were reconstructed using core
samples from the Bungo Channel (BC) in southwest Japan. The record of the Thalassionema spp. flux—the best index of fall primary productivity in the BC—indicated a multidecadal-scale duration with a low flux (1943–1982)
and those with a high flux (1913–1943 and 1982–2001); apparent shifts were recognized in 1943 and 1982. The shift in 1982
was also recognized in the flux records of other early summer to fall predominant genera in the BC and, previously, in the
biogenic silica records from a broad region of the southeast BC. This indicates that in our records, this shift reflects a
general trend in the primary production in the southeast BC. A comparison among the Thalassionema spp. flux records, meteorological data from an observatory adjacent to the core site, and the PDO index showed that the flux
records were more similar to the PDO index than the other meteorological records, which suggests that the multidecadal-scale
variability of the BC primary productivity may be associated with some marine-derived forcing. The bottom intrusions of nutrient-rich
water that upwelled from the shelf slope into the BC, the axis movement or the transport of the Kuroshio Current off the BC,
and a basin-scale wind stress in the North Pacific might play an important role in this forcing and mediate between the BC
primary productivity and the PDO. 相似文献
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Hodaka Kawahata Hideaki Ohshima Azumi Kuroyanagi 《Journal of Asian Earth Sciences》2011,40(6):430-1202
The Mixed Water Region (MWR) between the Oyashio Current and the Kuroshio Extension and surrounding terrestrial region have been affected by the global climatic change. The sedimentary core KR02-15 PC6 collected at 40°23.8871′N, 143°29.8663′E in the MWR provides detailed records of pollen and spores and alkenone sea surface temperature (SST) during the last 15 kyr, showing that both ocean and terrestrial environments have been appreciably linked. Abundance of Picea, a proxy for terrestrial cool climate, fluctuated, coinciding with the alkenone-SST change during the interstadial period (15.0–7.7 cal kyr B.P.). Alkenone-SST increased to the modern level at 8.0 cal kyr B.P. and remained almost constant until 2.0 kyr B.P. while the terrestrial warming still continued until 4.0 cal kyr B.P. The rainfall under terrestrial warm condition increased gradually from 7.9 cal kyr B.P. and rapidly from 2.0 cal kyr B.P. Both terrestrial and marine environments cooled down during the last 2 kyr possibly due to the intensified cold Oyashio Current. 相似文献
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Akinori Takami Teruo Kondo Azumi Kado Seiichiro Koda 《Journal of Atmospheric Chemistry》2001,39(2):139-153
Uptake coefficient of I2 on aqueous surfaces was measured by using an impinging flow method. Dependence of the uptake coefficient on the gas-liquid contact time and pH was investigated. The uptake coefficient was (3.7± 2.0)×10–4 at 120 ms, 293 K and pH = 5.6. In the alkaline region, the uptake coefficient was larger by one order of magnitude than that in the neutral and acidic region. The I2 uptake on KI solutions and synthetic sea water solutions was also measured. According to the analysis by the reactive uptake model, the uptake of I2 was shown to be mainly determined by liquid phase reactions and the accommodation coefficient of I2 on aqueous surfaces was estimated to be 0.01. 相似文献
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