A biodiversity evaluation framework for restoration of aquatic macrophyte communities in shallow lakes driven by hydrological process management

Macrophyte community diversity and composition respond to ecosystem conservation and local environmental factors. In this study, we developed a multidimensional diversity framework for macrophyte communities, including the taxonomic and functional alpha and beta diversity. We used the framework to explore the relationships among water level regimes and these diversity parameters in a case study of China's Baiyangdian Lake. Analysis of indicators of hydrologic alteration divided the water level from 1959 to 2019 into four regimes (dry, <6.42 m; low, 6.42–7.23 m; medium, 7.23–8.19 m; high, >8.19 m). Alpha and beta diversity were significantly higher in the medium regime than in the low and high regimes. Redundancy analysis indicated that the maximum water depth significantly affected taxonomic alpha diversity, and total nitrogen (TN) and chemical oxygen demand (COD) concentration significantly affected functional alpha diversity, respectively. Mantel tests showed that TN, Secchi depth (SD), and water depth in the high water level regime significantly increased the total beta diversity and turnover components. TN was the main factor that increased total taxonomic beta diversity. Water level regime mainly influenced interspecific relationships by changing the TN and COD concentration. The water level should be maintained between the medium and high water level regimes to promote restoration of the macrophyte community and improve ecosystem stability. The biodiversity evaluation framework would provide a deeper insight into the hydrological process management for restoration of aquatic macrophyte communities in shallow lakes.     

A novel coupled FDM-DEM modelling method for flexible membrane boundary in laboratory tests

Difficulties are involved in discrete element method (DEM) modelling of the flexible boundary, that is, the membranes covering the soil sample, which can be commonly found in contemporary laboratory soil tests. In this paper, a novel method is proposed wherein the finite difference method (FDM) and DEM are coupled to simulate the rubber membrane and soil body, respectively. Numerical plane strain and triaxial tests, served by the flexible membrane, are implemented and analysed later. The effect of the membrane modulus on the measurement accuracy is considered, with analytical formulae derived to judge the significance of this effect. Based on an analysis of stress-strain responses and the grain rotation field, the mechanical performances produced by the flexible and rigid lateral boundaries are compared for the plane strain test. The results show that (1) the effect of the membrane on the test result becomes more significant at larger strain level because the membrane applies additional lateral confining pressure to the soil body; (2) the tested models reproduce typical stress and volumetric paths for specimens with shear bands; (3) for the plane strain test, the rigid lateral boundary derives a much higher peak strength and larger bulk dilatation, but a similar residual strength, compared with the flexible boundary. The latter produces a more uniform (or ‘diffuse') rotation field and more mobilised local kinematics than does the former. All simulations show that the proposed FDM-DEM coupling method is able to simulate laboratory tests with a flexible boundary membrane.     

Hydrological management strategies for the control of algal blooms in regulated lowland rivers

Reservoirs of lowland floodplain rivers with eutrophic backgrounds cause variations in the hydrological and hydraulic conditions of estuaries and low-dam reservoir areas, which can promote planktonic algae to proliferate and algal bloom outbreaks. Understanding the ecological effects of variations in hydrological and hydraulic processes in lowland rivers is important for algal bloom control. In this study, the middle and lower reaches of the Han River, China, a typical regulated lowland river with a eutrophic background, are selected. Based on the effect of hydrological and hydraulic variability on algal blooms, a hydrological management strategy for river algal bloom control is proposed. The results showed that (a) differences in river morphology and background nutrient levels cause significant differences in the critical threshold flow velocities for algal bloom outbreaks between natural river and low-dam reservoir sections; there is no uniform threshold flow velocity for algal bloom control. (b) There are significant differences in the river hydrological/hydraulic conditions between years with and without algal blooms. The average river flow, water level and velocity in years with algal blooms are significantly lower than those in years without algal blooms. (c) For different river sections where algal blooms occur and to meet the threshold flow velocities, the joint operation of cascade reservoirs and diversion projects is an effective method to prevent and control algal blooms in regulated lowland rivers. This study is expected to deepen our understanding of the ecological significance of special hydrological processes and guide algal bloom management in regulated lowland rivers.     

A multiscale work-analysis approach for geotechnical structures

This paper presents a second-order work analysis in application to geotechnical problems by using a novel effective multiscale approach. To abandon complicated equations involved in conventional phenomenological models, this multiscale approach employs a micromechanically-based formulation, in which only four parameters are involved. The multiscale approach makes it possible a coupling of the finite element method (FEM) and the micromechanically-based model. The FEM is used to solve the boundary value problem (BVP) while the micromechanically-based model is utilized at the Gauss point of the FEM. Then, the multiscale approach is used to simulate a three-dimensional triaxial test and a plain-strain footing. On the basis of the simulations, material instabilities are analyzed at both mesoscale and global scale. The second-order work criterion is then used to analyze the numerical results. It opens a road to interpret and understand the micromechanisms hiding behind the occurrence of failure in geotechnical issues.     

Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

EFFECTS OF FISH OIL,DHA OIL AND LECITIHIN IN MICROPARTICULATE DIETS ON STRESS TOLERANCE OF LARVAL GILTHEAD SEABREAM（SPARUS AURATA）

The effects of natural fish oil,DHA oil and soybean lecithin in microparticulate diets on stress tolerance of larval gilthead seabream(Sparus aurata)were investigated after 15 days feeding trials.The tolerance of larval gilthead seabream to various stress factors such as exposure to air(lack of dissolved oxygen),changes in water temperature(low)and salinity(high) were determined.This study showed that microparticulate diet with natural fish oil and soybean lecithin was the most effective for in-creasing the tolerance of larval gilthead seabream to various stresses,and that microparticulate diet with natural fish oil and palmitic acid(16:0)was more effective than microparticulate diet with DHA oil and soybean lecithin.     

AN ILLUSTRATIVE STUDY ON LOCAL LANDSCAPEAND ITS LONG-TERM CHANGES BASED ON IKONOS AND HISTORICAL AERIAL PHOTO

Nowadays, the research works on landscape at fine scales using high-resolution images are uncommon.This research is based on the analysis of the combination of remote sensing data (1KONOS imagery acquired in 2002 and historical aerial photo taken in 1942). In the paper, the ecotopes in Qiujiadou and Xishao villages in Yixing City of Jiangsu Province in 1942 and 2002 were compared and landscape changes as well as the causes of the considerable changes were analyzed. It was found that the ecotope changes were at greater level in some aspects such as water surface and perennial vegetation coverage etc. This study at fine scale is globally significant for the rural areas, especially for the subsistence agricultural land, which occupies larger percentage in the earth. And it analyzes the structure of landscape based on a new landscape classification system--stratifications method.     

Discovery of 13 New Variable Stars in the Field of the Open Cluster NGC 2168 （M35）

A wide-field time-series CCD photometric survey of variable stars in the field of the open cluster NGC 2168 was carried out using the BATC Schmidt telescope. In total 13 new variable stars are discovered with three W UMa systems, one EA type and two EB type eclipsing binaries (one of them could be a W UMa system), and seven pulsating stars including three candidates of δScuti stars.     

Revisiting Sustainable Development of Dry Valleys in Hengduan Mountains Region

Dry valleys are a striking geographic landscape in Hengduan Mountains Region and are characterized by low rainfall, desert type of vegetation and fragile environment. Past efforts and resources have been concentrated mainly on rehabilitation of degraded ecosystem and fragile environment, particularly reforestation, while socio-economic development has been largely overlooked. Despite successes in pocket areas, the overall trend of unsustainability and environmental deterioration are continuing. It is important to understand that uplift of the Tibetan Plateau is the root cause of development of dry valleys, and development and formation of dry valleys is a natural process. Human intervention has played a secondary role in development of dry valleys and degradation of dry valleys though human intervention in many cases has speeded up environmental degradation of the dry valleys. It is important to understand that dry valleys are climatic enclaves and an integrated approach that combines rehabilitation of degraded ecosystems and socio-economic development should be adopted if the overall goal of sustainable development of dry valleys is to be achieved. Promotion of niche-based cash crops, rural energy including hydropower, solar energy, biogas and fuelwood plantation is recommended as the priority activities.     

Cytoplasmic regionalization in starfish oocyte occurrence and localization of cytoplasmic determinants responsible for the formation of archenteron and primary mesenchyme in starfish (asterias amurensis) oocytes

Starfish oocytes with intact germinal vesicles (GVs) were cut along desired planes with glass needles or ligated using silk thread loops into two parts and allowed to mature in vitro, and inseminated. The experimental results showed that (1) only the parts with GVs or partial GV contents (PGVCs) cleaved, those without any GV materials did not; but nucleated and non-nucleated fragments cut from mature eggs were able to divide; (2) the development of animal parts of oocytes containing GVs or PGVCs was like that of animal fragments of matured oocytes with female pronuclei; most of them gave rise to permanent blastulae, and just a few formed ectodermal vesicles with a little primary mesenchyme; (3) a large part of vegetal fragments with GVs or PGVCs, and the vegetal parts of mature eggs without female pronuclei developed into small but normal embryos; (4) the fragments containing GVs or PGVCs obtained from the oocytes along a plane parallel to the animal-vegetal (A-V) axis developed as normally as the halves (with or without female pronuclei) severed from mature eggs along the same axis. Based on the data above, it was concluded that (1) the non-chromatin materials in the oocyte GVs are indispensable for successful fertilization and cleavage of starfish eggs; (2) some factor (s) located asymmetrically in the vegetal hemispheres of starfish oocytes is (are) responsible for formation of the archenteron and primary mesenchyme. It is evident from the above findings that the oocyte cytoplasm of the starfish had already regionalized before the GV break-down. Contribution No. 1722 from the Institute of Oceanology, Academia Sinica