Sublittoral seaweed communities on natural and artificial substrata in a high-latitude coral community in South Africa

Coral mortality may result in macroalgal proliferation or a phase shift into an alga-dominated state. Subtidal, high-latitude western Indian Ocean coral communities at Sodwana Bay on the KwaZulu-Natal coast, South Africa, have experienced some mortality because of warm-water anomalies, storms and other causes, but the response of the macroalgae is unknown. We investigated the abundance and diversity of benthic algae on different hard natural substrata (dead digitate, brain and plate corals and beach rock) on Two-Mile Reef, Sodwana Bay. We also compared algal communities colonising ceramic, marble and pretreated ceramic tiles placed on the reef for six months. We identified 95 algae (14 Chlorophyta, 11 Phaeophyceae, 69 Rhodophyta and one cyanobacterium). Assemblages on natural and artificial substrata were dominated by the brown alga Lobophora variegata (Lamouroux) Womersley ex Oliveira and non-geniculate corallines (Rhodophyta, Corallinaceae). Cluster and ordination analyses revealed that the algae showed no affinity for particular substrata, whether natural or artificial. Algal cover was occasionally higher on rougher tiles and crustose corallines were significantly more abundant on marble than ceramic tiles. Two-Mile Reef had 23.1% dead and 48.4% live scleractinian coral cover, where dead corals were colonised indiscriminately by many small algal species, but there was no evidence of algal proliferation. The results provide a baseline for monitoring this high-latitude reef system.     

Phytoplankton Spatial Variability in the River-Dominated Estuary,Apalachicola Bay,Florida

In shallow estuaries with strong river influence, the short residence time and pronounced gradients generate an environment for plankton that differs substantially in its dynamics from that of the open ocean, and the question arises “How is phytoplankton biomass affected?” This study assesses the small-scale spatial and temporal distribution of phytoplankton in Apalachicola Bay, a shallow bar-built estuary in the Florida Panhandle. Phytoplankton peaks were characterized to gain insights into the processes affecting spatial heterogeneity in biomass. Chlorophyll a (Chl a) distribution at 50-m spatial resolution was mapped using a flow-through sensor array, Dataflow©, operated from a boat that sampled four transects across the bay every 2 weeks for 16 months. Chl a peaks exceeding background concentrations had an average width of 1.3?±?0.7 km delineated by an average gradient of 3.0?±?6.0 μg Chl a L^?1 km^?1. Magnitude of E-W wind, velocity of N-S wind, tidal stage, and temperature affected peak characteristics. Phytoplankton contained in the peaks contributed 7.7?±?2.7% of the total integrated biomass observed along the transects during the study period. The river plume front was frequently a location of elevated Chl a, which shifted in response to river discharge. The results demonstrate that despite the shallow water column, river flushing, and strong wind and tidal mixing, distinct patchiness develops that should be taken into consideration in ecological studies and when assessing productivity of such ecosystems.     

Transparent Exopolymer Particles (TEP) in a River-Dominated Estuary: Spatial–Temporal Distributions and an Assessment of Controls upon TEP Formation

Transparent exopolymer particle (TEP) distributions were examined in North Carolina’s Neuse River Estuary (NRE) over a 1-year period, and experiments were conducted to examine controls upon TEP formation. TEP-carbon averaged 16% of the total organic carbon pool in the NRE. From May to early August 2007, TEP ranged from 805 to 1,801 μg xanthan gum (XG) L⁻¹. A large phytoplankton bloom peaked in early August and then subsequently declined. Within 2 weeks of the bloom’s peak, TEP concentrations increased to >3,500 μg XG L⁻¹ and remained elevated through mid-December. Decreasing water temperatures and enhanced retention due to drought conditions in Fall 2007 likely aided in the persistence of TEP concentrations during this timeframe. Thereafter, TEP decreased coincident with increased river flow and flushing of the estuary that began in January 2008, and TEP remained low (ranging from 991 to 1,712 μg XG L⁻¹) until the end of April 2008. TEP was positively correlated with salinity, suggesting that cation availability (which co-varies with salinity and stabilizes the structural integrity of TEP) may play a role in estuarine TEP formation. In two cation addition experiments using water from the mesohaline region, TEP concentrations tended to be higher in treatments with vs. without cations added. Statistically significant relationships were also found between in situ TEP concentrations and pH, mixing, and temperature, suggesting that a complex suite of environmental parameters affect TEP formation and its distribution in estuaries.     

Dredging-induced nutrient release from sediments to the water column in a southeastern saltmarsh tidal creek

Dredging is a large-scale anthropogenic disturbance agent in coastal and estuarine habitats that can profoundly affect water quality. We examined the impact of a small-scale dredging operation in a salt marsh in South Carolina by comparing nutrient levels (NH(4)(+), NO(x), PO(4)(-)) and total suspended solid concentrations before and during dredging activities. Nutrient enrichment was evaluated within the context of tidal, seasonal, and inter-annual variability by using long-term water chemistry data provided by the North Inlet-Winyah Bay National Estuarine Research Reserve. The conditions of the dredging permit (i.e., its relatively small scale), the season chosen for the work (fall-winter), the nature of the sediments dredged (coarse-grained), and the amount of natural variability in the estuary's water chemistry (even on a daily time-scale) all minimized the impact of the dredging activities. Results of this study will add to the limited body of empirical data that should be considered in evaluating future dredging permit applications related to shallow estuarine waterways.     

辉钼矿Re-Os同位素定年方法的改进与应用

公认的Re-Os同位素定年代表物辉钼矿,目前已在金属硫化物矿床年代学研究领域获得了广泛的应用。本研究采用浓HNO₃分解辉钼矿样品,大大地简化了Re和Os的化学制备过程,并根据辉钼矿中正常Os含量水平相对放射成因¹⁸⁷Os可以忽略的特点,以正常Os标准为稀释剂,实现了仪器测量过程中Os同位素质量分馏的在线校正,改善了分析数据的质量。该方法经辉钼矿国家标准物质进行验证,获得了满意的Re、Os含量及Re-Os年龄数据,并且在南岭地区与连阳复式岩体相关的姓坪夕卡岩型钼矿床成矿年龄研究中获得了成功应用。在实际应用中,为了获得有意义的能反映真实地质事件的年龄数据,辉钼矿样品不仅要有足够的取样量,而且还要保证粒度细、混合均匀。     

Estuarine Phytoplankton Responses to Hurricanes and Tropical Storms with Different Characteristics (Trajectory,Rainfall, Winds)

We examined the short-term (<1 month post-storm) impact of storms [Tropical Storm (TS) Helene in 2000, Hurricane (H) Isabel in 2003, H Alex, Tropical Depression (TD) Bonnie and TS Charley in 2004] varying in their trajectory, wind and rainfall characteristics, on water column structure, nutrients, and phytoplankton biomass in North Carolina’s Neuse R. Estuary (NRE). Data are presented from two sampling programs, ModMon (biweekly) and FerryMon (measurements made every 3 min daily). Helene’s winds mixed the previously stratified water column, delivering sediment-bound nutrients to the euphotic zone, and localized freshwater input from Helene was also evident. Mean chlorophyll a concentrations in the mesohaline portion of the NRE, where N was strongly limiting before the storm (molar DIN:DIP < 1), more than doubled after the storm. Unlike with Helene, the water column was well mixed before passage of Isabel, and nutrient concentrations were high. As a result, minimal impact on phytoplankton biomass was detected despite Isabel’s high winds and significant freshwater input. In fact, conditions became less favorable for phytoplankton growth after the storm. Alex was fast moving and relatively small, but its winds were sufficient to mix the water column. Although data from ModMon suggest that chlorophyll a was only slightly higher after passage of Alex, FerryMon detected an ephemeral bloom that was missed by ModMon. Overall, these results suggest that relatively small tropical storms and hurricanes can lead to significant increases in phytoplankton biomass. However, the phytoplankton response depends on both the characteristics of a particular storm and the physical–chemical conditions of the water column before storm passage. Finally, the ephemeral bloom that developed as a result of Alex, the strong response of phytoplankton in the mesohaline portion of the estuary to nutrient inputs, and their patchiness on several other occasions suggests that storms may create “hot spots” for trophic transfer and biogeochemical dynamics in estuaries. Adaptive sampling is necessary to capture these features and to fully understand the impact of perturbations such as storms on estuarine ecosystem functioning.     

Using an unbaited stationary video system to investigate the behaviour and interactions of bull sharks Carcharhinus leucas under an aquaculture farm

Bull sharks Carcharhinus leucas are common along the coast of Reunion Island (South-West Indian Ocean) and were suspected to aggregate in the vicinity of an aquaculture farm in Saint-Paul Bay on the west coast. In order to understand the behaviour and interaction of bull sharks near aquaculture cages at Saint-Paul Bay, we deployed an experimental unbaited stationary video camera. From 175 hours of recording during daylight hours from March to April 2012, eight individual female bull sharks (seven adults and one immature) were identified based on their natural markings. These sharks were resighted between 3 and 45 times. Residency analysis revealed site attachment under the aquaculture cages for at least three individuals over the course of the study. Recorded behaviours included intraspecific social interactions such as synchronised swimming. Social interactions and relatively strong paired associations for two pairs of females suggest some level of sociality among bull sharks around Reunion Island. Overall, our results demonstrate the utility of unbaited video systems to monitor the behaviour of adult coastal sharks.     

Biogeochemistry of a River-Dominated Estuary Influenced by Drought and Storms

Increased frequency and severity of droughts, as well as growing human freshwater demands, in the Apalachicola-Chattahoochee-Flint River Basin are expected to lead to a long-term decrease in freshwater discharge to Apalachicola Bay (Florida). To date, no long-term studies have assessed how river discharge variability affects the Bay’s phytoplankton community. Here a 14-year time series was used to assess the influence of hydrologic variability on the biogeochemistry and phytoplankton biomass in Apalachicola Bay. Data were collected at 10 sites in the bay along the salinity gradient and include drought and storm periods. Riverine dissolved inorganic nitrogen and phosphate inputs were correlated to river discharge, but chlorophyll a (Chl a) was similar between periods of drought and average/above-average river discharge in most of the Bay. Results suggest that the potentially negative impact of decreased riverine nutrient input on Bay phytoplankton biomass is mitigated by the nutrient buffering capacity of the estuary. Additionally, increased light availability, longer residence time, and decreased grazing pressures may allow more Chl a biomass to accumulate during drought. In contrast to droughts, tropical cyclones and subsequent increases in river discharge increased flushing and reduced light penetration, leading to reduced Chl a in the Bay. Analysis of the time series revealed that Chl a concentrations in the Bay do not directly mirror the effect of riverine nutrient input, which is masked by multiple interacting mechanisms (i.e., nutrient loading and retention, grazing, flushing, light penetration) that need to be considered when projecting the response of Bay Chl a to changes in freshwater input.     

Quantification of Vibrio vulnificus in an Estuarine Environment: a Multi-Year Analysis Using QPCR

Using a refined quantitative polymerase chain reaction assay, we compared surface and bottom water concentrations of Vibrio vulnificus with total Vibrio spp. concentrations, fecal indicator bacteria (Escherichia coli and Enterococcus sp.), and environmental parameters (salinity, temperature, chlorophyll a, and turbidity) over 4 years at a mesohaline site in the Neuse River Estuary in North Carolina. V. vulnificus was not detected when water temperature was below 20 °C and 93 % of positive samples were from salinities 10–20 psu. V. vulnificus was detected in 50 % of summer samples with peak concentrations in summer bottom waters. Significant positive correlations for V. vulnificus and temperature, salinity, and bacterial group abundance were found. Significant negative correlations with chlorophyll a were also observed. Interannual comparisons indicated a significant decrease in mean V. vulnificus since 2005, corresponding with increasing salinity due to severe drought conditions in 2007 and 2008. Total Vibrio spp. abundance also decreased in 2007 but returned to the previously observed abundance by 2008. Although a significant positive relationship between total Vibrio spp. and V. vulnificus was documented, interannual comparisons indicate that total Vibrio spp. densities may not be indicative of V. vulnificus in all environmental conditions and that long-term drought conditions may alter community composition.