Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

观驾山—黑岛一带信息量法找金研究

本文以成矿地质模型为依据，对观驾山—黑山岛一带的化探、物探、遥感及地质等信息变量进行了详细的分析，选取了１０个信息变量２４个标志状态；在此基础上，运用信息量法对研究区内４５８个单元的信息量值进行计算，并由此确定了研究范围内的成矿远景区。事实表明，研究区信息量成矿预测是非常有效的     

Microwave Type III Burst Group in the 1993 October 2 Flare

Microwave Type III bursts with positive frequency drifting rate were found by Stahli and Benz (1987) for first time. Type III events are especially interesting because they are well-known to be signatures of electron beams in coronal plasma, and they are effective means for diagnosting of source plasma. A microwave burst consisting of some microwave type III burst groups was registered at Beijing Astronomical Observatory with the 2545–2645–2840 MHz synchronous observing system. The distributions of frequency drifting rate, half power duration, and intensity for each impulse in the groups have been statistically analysed. From this analysis, some important parameters for the dynamic process in the flare are deduced and discussed.     

Dynamic analysis of three-dimensional marine cables

Marine cables are widely used in a variety of offshore activities. In this paper, a numerical approach is presented which is capable of predicting the dynamic behaviour of three-dimensional marine cables. The solution of this problem is of practical importance in the estimation of dybamic loading and motion, and thus has direct application to the enhancement of safety and the effectiveness of the offshore activities. The approach is based upon the lump-mass-and-spring model and the finite difference method. Mathematical analysis is provided on the basic characteristics of the model and the numerical stability. A numerical example is given to demonstrate the validity of the approach.     

富氏谱分析法模拟地壳垂直运动的地学依据及其策应力模型

应用地壳波浪与镶嵌构造学说对富氏谱分析法提取地壳垂直形变信息的科学性做了地质学意义上的阐释 ,并提出了根据多期形变资料提取特定波段上构造策应力的数学模型     

The Statistical Analysis of Migration of Strong Earthquakes-Taking the North China Region as an Example

The migration of strong earthquakes is an important research topic because the migration phenomena reflect partly the seismic mechanism and involve the prediction of tendency of seismic activity. Research on migration of strong earthquakes has mostly focused on finding the phenomena. Some attempts on getting regularity were comparatively subjective. This paper suggests that there should be indices of migration in earthquake dataset and the indexes should have statistical meaning if there is regularity in the migration of strong earthquakes. In this study, three derivative attributes of migration, i.e., migration orientation, migration distance and migration time interval, were statistically analyzed. Results in the North China region show that the migration of strong earthquakes has statistical meaning. There is a dominant migration orientation (W by S to E by N), a dominant distance (≤100km and on the confines of 300～700km), and a dominant time interval (≤1a and on the confines of 3～4a). The results also show that the migration will differ slightly with different magnitude range or earthquake activity phase.     

星际A型CH_3CH－H_2含超精细能级的碰撞跃迁速率系数的理论计算

本文作者利用ＩＯＳ近似模型，计算了星际分子云条件下Ａ型ＣＨ３ＣＮ－Ｈ２含超精细能级的碰撞跃迁速率系数。温度范围是２０Ｋ－１４０Ｋ。为研究分子云与恒星形成区的物理、化学性质提供了有用的基础分子数据。     

Automated Separation of Stars and Normal Galaxies Based on Statistical Mixture Modeling with RBF Neural Networks

For LAMOST, the largest sky survey program in China, the solution of the problem of automatic discrimination of stars from galaxies by spectra has shown that the results of the PSF test can be significantly refined. However, the problem is made worse when the redshifts of galaxies are not available. We present a new automatic method of star/(normal) galaxy separation, which is based on Statistical Mixture Modeling with Radial Basis Function Neural Networks (SMM-RBFNN). This work is a continuation of our previous one, where active and non-active celestial objects were successfully segregated. By combining the method in this paper and the previous one, stars can now be effectively separated from galaxies and AGNs by their spectra-a major goal of LAMOST, and an indispensable step in any automatic spectrum classification system. In our work, the training set includes standard stellar spectra from Jacoby's spectrum library and simulated galaxy spectra of EO, SO, Sa, Sb types with redshift ranging from 0 to 1     

Timing of UHP metamorphism in the Hong’an area,western Dabie Mountains,China: evidence from zircon U–Pb age,trace element and Hf isotope composition

The Hong’an area (western Dabie Mountains) is the westernmost terrane in the Qinling-Dabie-Sulu orogen that preserves UHP eclogites. The ages of the UHP metamorphism have not been well constrained, and thus hinder our understanding of the tectonic evolution of this area. LA-ICPMS U–Pb age, trace element and Hf isotope compositions of zircons of a granitic gneiss and an eclogite from the Xinxian UHP unit in the Hong’an area were analyzed to constrain the age of the UHP metamorphism. Most zircons are unzoned or show sector zoning. They have low trace element concentrations, without significant negative Eu anomalies. These metamorphic zircons can be further subdivided into two groups according to their U–Pb ages, and trace element and Lu–Hf isotope compositions. One group with an average age of 239 ± 2 Ma show relatively high and variable HREE contents (527 ≥ Lu_N ≥ 14) and ¹⁷⁶Lu/¹⁷⁷Hf ratios (0.00008–0.000931), indicating their growth prior to a great deal of garnet growth in the late stage of continental subduction. The other group yields an average age of 227 ± 2 Ma, and shows consistent low HREE contents and ¹⁷⁶Lu/¹⁷⁷Hf ratios, suggesting their growth with concurrent garnet crystallization and/or recrystallization. These two groups of age are taken as recording the time of prograde HP to UHP and retrograde UHP–HP stages, respectively. A few cores have high Th/U ratios, high trace element contents, and a clear negative Eu anomaly. These features support a magmatic origin of these zircon cores. The upper intercept ages of 771 ± 86 and 752 ± 70 Ma for the granitic gneiss and eclogite, respectively, indicate that their protoliths probably formed as a bimodal suite in rifting zones in the northern margin of the Yangtze Block. Young Hf model ages (T _DM1) of magmatic cores indicate juvenile (mantle-derived) materials were involved in their protolith formation. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.