Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

观驾山—黑岛一带信息量法找金研究

本文以成矿地质模型为依据，对观驾山—黑山岛一带的化探、物探、遥感及地质等信息变量进行了详细的分析，选取了１０个信息变量２４个标志状态；在此基础上，运用信息量法对研究区内４５８个单元的信息量值进行计算，并由此确定了研究范围内的成矿远景区。事实表明，研究区信息量成矿预测是非常有效的     

Prediction Test for the Two Extremely Strong Solar Storms in October 2003

In late October and early November 2003, a series of space weather hazard events erupted in solar-terrestrial space. Aiming at two intense storm (shock) events on 28 and 29 October, this paper presents a Two-Step method, which combines synoptic analysis of space weather–`observing’ and quantitative prediction – ‘palpating’, and uses it to test predictions. In the first step, ‘observing’, on the basis of observations of the source surface magnetic field, interplanetary scintillation (IPS) and ACE spacecraft, we find that the propagation of the shock waves is asymmetric and northward relative to the normal direction of their solar sources due to the large-scale configuration of the coronal magnetic fields, and the Earth is located near the direction of the fastest speed and greatest energy of the shocks. Being two fast ejection shock events, the fast explosion of extremely high temperature and strong magnetic field, and background solar wind velocity as high as 600 and 1000 km s⁻¹, are also helpful to their rapid propagation. According to the synoptic analysis, the shock travel times can be estimated as 21 and 20 h, which are close to the observational results of 19.97 and 19.63 h, respectively. In the second step, ‘palpating’, we adopt a new membership function of the fast shock events for the ISF method. The predicted results here show that for the onset time of the geomagnetic disturbance, the relative errors between the observational and the predicted results are 1.8 and 6.7%, which are consistent with the estimated results of the first step; and for the magnetic disturbance magnitude, the relative errors between the observational and the predicted results are 4.1 and 3.1%, respectively. Furthermore, the comparison among the predicted results of our Two-Step method with those of five other prevailing methods shows that the Two-Step method is advantageous in predicting such strong shock event. It can predict not only shock arrival time, but also the magnitude of magnetic disturbance. The results of the present paper tell us that understanding the physical features of shock propagation thoroughly is of great importance in improving the prediction efficiency.     

Microwave Type III Burst Group in the 1993 October 2 Flare

Microwave Type III bursts with positive frequency drifting rate were found by Stahli and Benz (1987) for first time. Type III events are especially interesting because they are well-known to be signatures of electron beams in coronal plasma, and they are effective means for diagnosting of source plasma. A microwave burst consisting of some microwave type III burst groups was registered at Beijing Astronomical Observatory with the 2545–2645–2840 MHz synchronous observing system. The distributions of frequency drifting rate, half power duration, and intensity for each impulse in the groups have been statistically analysed. From this analysis, some important parameters for the dynamic process in the flare are deduced and discussed.     

Isotopic evidence for holocene climatic changes in Bosten Lake, Southern Xinjiang, China

Based on the δ¹³C and δ¹⁸O fluctuation of lacustrine carbonate, CaCO₃ content and sporo-pollen data, a palaeoclimatic history of Bosten Lake during the Holocene has been outlined, several stages of climatic changes are divided, and the following result es are obtained: (1) Palaeoclimatic changes revealed by carbonate isotope around Bosten Lake are basically identical with that revealed by other geological records in Xinjiang. Environmental changes presented apparent Westlies Style model: during cold period, relative humidity increased, δ¹⁸O, δ¹³C and CaCO₃ appeared low; but in warm periods, the dry regime aggravated. (2) The temperature reflected by δ¹⁸O exist evident features being increase in the late period during the Holocene. Together with the δ¹³C, pollen and CaCO₃ analyses, several cold and warm phases which are of broad regional significance can be identified. The warm peaks occurred at about 11.0 ka B.P., 9.4 ka B.P., 7.5 ka B.P., 5.0 ka B.P., 3.0 ka B.P. and 2.0 ka B.P.; the cold peaks at 11.5 ka B.P., 10.5 ka B.P., 8.8 ka B.P., 5.5 ka B.P., 3.3 ka B.P., 2.2 ka B.P. and 1.5 ka B.P.. (3) Several climatic events with the nature of “abrupt climatic changes” are revealed in the periods of 11.0 ka B.P. −10.5 ka B.P., 9.4 ka B.P. −8.8 ka B.P., 5.5 ka B.P. −5.0 ka B.P. and 2.0 ka B.P. −1.5 ka B.P.. (4) The results show that carbonate isotopic record of lacustrine sediment in arid area is very sensitive to climatic changes, and may be play a very important role in understanding the features and mechanism of palaeoclimatic changes.     

北部湾北部海上油田发现历程

北部湾北部海上油田的开发工作始于５０年代，６０～７０年代作了大量地质工作。改革开放后，油气勘查取得突破性进展。累计发现油气构造６个，含油面积超过４０ｋｍ２，石油地质储量约４亿ｔ，已开发油田２个，揭开我国南方油气资源开发序幕     

彗星等离子体某些特征的讨论

本文讨论了彗星中尘埃粒子的充电机制 ,带电特性和平衡电势的变化规律 ,分析了彗星尘埃的破碎特性和临界半径 ,得出了很有意义的结果     

南海北缘北东东向构造活动性研究

从地形地貌特征、重磁地球物理场、深部构造与断裂构造以及地震活动、震源机制等多方面论述北东东向构造是南海北缘的主要活动构造。对于长期以来认为新华夏系北东向构造是该区主要活动构造的观点来说，这是一种新的学术思想。     

富氏谱分析法模拟地壳垂直运动的地学依据及其策应力模型

应用地壳波浪与镶嵌构造学说对富氏谱分析法提取地壳垂直形变信息的科学性做了地质学意义上的阐释 ,并提出了根据多期形变资料提取特定波段上构造策应力的数学模型