Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

BORDER PORT MANZHOULh URBAN FUNCTION AND SPACE DEVELOPMENT

Manzhouli is the largest land port city on the Sino-Russia border, transit cargo through the land port amount-ed to 5.95 million tons, transit tourists were 304 500 in 2000. It stands at the joint place of China, Mongolia and Russi-a, faces to Siberia area of Russia, receives direct support from the Northeast China and Bohai Sea Rim Area, and possess-es priorities in geographical location, land port infrastructure, water resources, coal resources, tourist resources andgreat potentiality in economic cooperation with Russia. The future urban function is a key port on the First Eurasia Continen-tal Bridge. Manzhouli Port will keep its first place between China and Russia land transport, and it is forecasted that thetransit amount through Manzhouli Port will go up to 10 million tons in 2005 and 20 million tons in 2010. It will be construct-ed to be a trade center of the peripheral area extending to Russia and Mongolia, a key export-oriented processing industri-al zone supported by industries such as export processing industries, export agriculture, trade services, technology trad-ing and the other service industries. It keeps being a well-known touring city for trade, shopping, sightseeing, vocation,local food, recreation and cultural events. To build Manzhouli Export Processing Industry Zone will improve city econom-ic structure, and the main sectors are organic food processing, livestock products processing, garment and furniture indus-try. Moreover, Manzhouli Export Processing Industry Zone will eventually be upgraded to be a border free trade zone.The city functional transition will inevitably affect urban spatial restructure and its expansion. The city space transforma-tion will develop as such: one development axis of No. 301 highway paralleling with Bin - Zhou(Harbin - Manzhouli) rail-way which cuts through central part of Manzhouli City, and links Zhalainuocr District with central city; three urban unitsincluding central city, Zhalainuocr District and Manzhouli Interchange Trade Zone; cohesion with Aoerjin and Cuogangpastures; regional dual-nuclei structure of Hailaer City and Manhzouli City; and the Manzhouli-Zabaykalsk Free TradeZone.     

Discovery of 13 New Variable Stars in the Field of the Open Cluster NGC 2168 （M35）

A wide-field time-series CCD photometric survey of variable stars in the field of the open cluster NGC 2168 was carried out using the BATC Schmidt telescope. In total 13 new variable stars are discovered with three W UMa systems, one EA type and two EB type eclipsing binaries (one of them could be a W UMa system), and seven pulsating stars including three candidates of δScuti stars.     

观驾山—黑岛一带信息量法找金研究

本文以成矿地质模型为依据，对观驾山—黑山岛一带的化探、物探、遥感及地质等信息变量进行了详细的分析，选取了１０个信息变量２４个标志状态；在此基础上，运用信息量法对研究区内４５８个单元的信息量值进行计算，并由此确定了研究范围内的成矿远景区。事实表明，研究区信息量成矿预测是非常有效的     

青藏块体东北缘断层形变与中强地震

对祁连山－海原断裂带近期断层形变特征进行了初步研究，发现多场地，大范围的断层活动异常是中等强度地震发生的显著背景，并且往往与大陆地震活动的阶段性总体状况相呼应；区域形变存在明显的特征量，包括特征形态和特征时间，同一场地在不同地震前的异常特征具有重复性，但会受到背景差异显著的不同地震的影响，目前形变状况表明研究区仍具有发生中强地震的地壳运动背景。     

BRINGING FORWARD OF THE CONCEPT OF COGIS AND ITS ARCHITECUTRE RESEARCH

Based on the analysis of the development of GIS technology and application,this paper brought forward the concept of GoGIS,namely Cooperative GIS ,CoGIS is GIS facing group-users and supporting human-human interaction,which makes it differ from the former GISs,Then,the characteristics of general Computer Spport Cooperative Work (CSCW)applications and the complexity of Geographic Information Science were analyzed,and the conclusion the CoGIS was not a simple GIS layer on CSCW was reached,Further,this paper gaver the hierarchical architecture of CoGIS,and analyzed the coperative platform in detail from the following:1)basic elements;2) collaboration patterns;3) cooperation control mechanism;4) synchronization mechanism;5) security and 6) group communication and so on.With those,the problems about GIS applications are discussed,such as 1)distributed multi-source GIS information and knowledge sharing platform;2)the fusion and visualization of GIS information;3)virtual reality cooperative modeling;4) dymamic simulation;5)expert system and 6) decision-making.Finally,this paper analyzed CoGIS application mode in brief.     

Microwave Type III Burst Group in the 1993 October 2 Flare

Microwave Type III bursts with positive frequency drifting rate were found by Stahli and Benz (1987) for first time. Type III events are especially interesting because they are well-known to be signatures of electron beams in coronal plasma, and they are effective means for diagnosting of source plasma. A microwave burst consisting of some microwave type III burst groups was registered at Beijing Astronomical Observatory with the 2545–2645–2840 MHz synchronous observing system. The distributions of frequency drifting rate, half power duration, and intensity for each impulse in the groups have been statistically analysed. From this analysis, some important parameters for the dynamic process in the flare are deduced and discussed.     

Application of Grain Size Analysis to Study Sedimentary Environment of Sand Dune in the Vicinity of Tumen River

The dunes in estuary of Tumen River in China lie to the area between the Jiushaping and Fangchuan in the left bank of Tumen River (Fig.1). The dis-tance is about 15～20 km between the dunes and the coast. It ranges from China to D. P. R. Korea and Russia. The range of the height of dunes is about 15～20 m and the width is 100～200 m. By the re-connaissance, we protracted 9 section planes of the dunes and collected 40 sand samples of the dunes, and identified the sedimentary environment …