Ecological–Microbiological Studies of Lake Beloe in Winter and Spring with the Use of Innovation Test-Systems

Water Resources - Chlorophyll a concentration, the total abundance of bacteria, the number of bacterial cells with active metabolism, and the abundance of saprotrophic bacteria were studied in the...     

State of heterotrophic bacterioplankton of Yenisei estuary and the zone of Ob–Yenisei discharge in autumn in relation with environmental factors

In September 2011, variations of the total abundance of bacteria were studied, including, for the first time, the abundance of bacteria with intact membranes and active electron transport chains, as well as chlorophyll a concentrations and some hydrological and hydrochemical characteristics of water in Yenisei estuary and the zone of Ob–Yenisei river discharge. The study found abundant, viable, and actively functioning bacterioplankton to exist in the region. The absolute values of all microbiological characteristics were found to decrease with the distance from the freshened waters of Yenisei estuary toward the seawater of the Ob–Yenisei river discharge, while the percentage of bacteria with intact membranes and actively functioning microorganisms in bacterioplankton increased in the same direction. Positive correlations were found to exist between the values of all examined microbiological characteristics and the concentration in water of chlorophyll a, silicium, organic nitrogen, and oxygen, as well as water temperature. The same biological characteristics showed negative correlations with water salinity and phosphates. The spatial distribution of bacteria in the examined water body was supposed to be due mostly to the intensity of hydrodynamic processes, rather than the biogenic matter content of water.     

Current state of heterotrophic bacterioplankton in the Kosinskie Lakes

From December 2009 to December 2010, the total abundance of bacteria, the abundance of bacteria with electron transport chain, the abundance of viable saprotrophic bacteria, and the concentration of chlorophyll a were studied in water samples from lakes Svyatoe, Beloe, and Chernoe in Kosino-Ukhtomskoe district, Moscow City. The abundance of bacteria with active electron transport chain (CTC+bacteria) and its seasonal variations in Kosinskie Lakes are studied for the first time. Those lakes were found to contain well-developed and actively functioning heterotrophic bacterioplankton, the abundance of which have increased considerably since the 1930s–1940s. The seasonal dynamics of bacterioplankton has also changed. The largest absolute abundance of CTC+bacteria in lakes Svyatoe and Beloe was recorded in summer and autumn (from August to November). The abundance peak of CTH+bacteria in Lake Svyatoe was recorded in September and that in Lake Beloe, in October. The abundance of CTC+bacteria also showed a peak in April. In Lake Chernoe, the abundance of CTC+bacteria was much less than in lakes Svyatoe and Beloe; its maximums were recorded in December 2009 and April 2010. The share of CTC+bacteria in the total abundance of bacteria was 2.4–19.2% in Lake Svyatoe, 1.8–63.0% in Lake Beloe, and 0.96–22.5% in Lake Chernoe. Significant correlations were found to exist in all three lakes between the abundance of active bacterioplankton fraction and chlorophyll a content of water.     

Distribution of bacterioplankton with active metabolism in waters of the St. Anna Trough,Kara Sea,in autumn 2011

The distribution of bacterioplankton with active electron transport chains, as well as bacteria with intact cell membranes, was investigated for the first time in the region of St. Anna Trough in the Kara Sea. The average number of bacteria with active electron transport chains in the waters of the St. Anna Trough was 15.55 × 10³ cells mL^–1 (the limits of variation were 1.06–92.17 × 10³ cells mL^–1). The average number of bacteria with intact membranes was 33.46 × 103 cells mL^–1 (the limits of variation were 6.78 to 103.18 × 10³ cells mL^–1). Almost all bacterioplankton microorganisms in the studied area were potentially viable, and the average share of bacteria with intact membranes was 92.1% of the total number of bacterioplankton (TNB) (the limits of variation were 76.2 to 98.4%). The share of bacteria with active metabolisms was 38.2% of the TNB (the limits of variation were 5.6–93.4%). The shares of the bacteria with active metabolisms were maximum in areas with the most stable environmental conditions (on the shelf and in deep water), whereas on the slope, where the gradients of water temperature and salinity were maximum, these values were lower.     

Spatial Variability of Phytoplankton Primary Production Characteristics in the North Atlantic in Summer 2013

Oceanology - The spatial variability of phytoplankton primary production characteristics has been studied along transects between the Shetland Islands and Iceland (transect I) and along 59.5°...     

Bacterioplankton in the northeastern part of the Black Sea during the summer and autumn of 2005

On the basis of luminescent microscopy using a DAPI dye, the abundance, morphometric characteristics, and biomass of bacteria in the coastal waters off the northeastern part of the Black Sea in 2005 were considered. The material was collected in June, September, and October along the cross section from Golubaya Bay towards the open part of the sea. An analysis of the microorganism development level showed that the bacteria achieved their maximal abundance in September, amounting, in the closest near-shore area, to 2435 × 10³ cells/ml, or 276 mg/m³ of wet weight; the lowest values were characteristic of October. During all the survey periods, coccal forms were prevailing among the microorganisms; regardless of the season, their fraction was maximal at the coastal stations. The greatest prevalence of coccal forms was characteristic of October (83%). The average sizes of the bacterial cells in 2005 varied within 0.073–0.257 μm³. The highest values of the bacterial abundance and biomass, almost in all the cases, were found at coastal stations; with the distance from the coast, a decrease in the bacterioplankton abundance was observed. A comparison of methodically similar data on the concentration of bacteria (dying with acridine orange and DAPI) from 1985 till now shows a trend of the annual growth in the bacterial abundance.