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1.
Trace metal-binding proteins in marine molluscs and crustaceans 总被引:1,自引:0,他引:1
Some marine invertebrates, such as the American oyster, Crassostrea virginica, the blue crab, callinectes sapidus and the American lobster, Homarusamericanus, concentrate trace metals in their tissues. The occurrence of metallothionein, a low molecular weight, sulfur-containing metal-binding protein, has been correlated with elevated levels of trace metals in these organisms. It is our hypothesis that, whilst metallothioneins are involved in the sequestration of elevated levels of trace metals, they primarily function in regulating normal metal metabolism. In this paper we describe recent field and laboratory experiments designed to examine how oysters, blue crabs and lobsters manage accumulated cadmium, copper and zinc. The possible rôles of metallothionein, as well as the tissue distribution of metals, are emphasized. Metallothioneins were found in all animals we examined; concentrations of metallothioneins and the the amount of bound trace metals are related to time of exposure. We have also demonstrated that the laboratory developed metallothionein models for blue crabs and lobsters may be useful in predicting the partitioning of trace metals in animals living in environments polluted with trace metals. 相似文献
2.
The accumulation of cadmium from seawater by the blue crab, Callinectes sapidus, was studied as a function of metal concentration and exposure time, with special emphasis on cadmium-binding proteins. Cadmium was found, in decreasing order of magnitude, in gills, digestive gland and hemolymph. When exposed to 0·5 ppm cadmium for 2–24h, virtually all of the cadmium in the cytosolic fraction of the gill was associated with a low molecular weight (LMW) cadmium-binding protein (MW 8000). However, after 48h of exposure only 50% of the cadmium in the cytosol was bound to this protein. The rest wasfound to be associated with proteins of a molecular weight of 300 000 and 60 000. This pattern of cadmium distribution did not change over a 12-day depuration period. Similar results were obtained upon exposure to 0·1 ppm cadmium. The pattern of cadmium accumulation in the cytosolic fraction of the digestive gland was in marked contrast to that observed for the gill. Initially, the cadmium was distributed over three low molecular weight fractions. During depuration the distribution of cadmium changed and all of the metal became bound to a low molecular weight protein (MW 9000). The cadmium concentrations in the gill and digestive gland remained essentially constant during depuration (12 days). The LMW cadmium-binding proteins were purified by a combination of gelpermeation and ion-exchange chromatography. Their molecular weight, spectral properties and amino acid composition are characteristic of the vertebrate metallothioneins. During exposure to cadmium the metal rapidly appeared in the hemolymph, mainly associated with hemocyanin. During depuration cadmium was transferred from the hemolymph to the digestive gland, demonstrating that hemocyanin acts as a carrier in trace metal transport. 相似文献
3.
This paper focuses on different ways of characterizing the solar radiative regime of a day and the stability of this regime. The days may be stratified in classes of cloud shade, observed total cloud cover amount, daily averaged clearness index, and fractal dimension of the solar global irradiance signal. A new Boolean parameter related to solar irradiance fluctuation is defined, namely the sunshine stability number. The time averaged value of the sunshine stability number is used for the characterization of the radiative regime stability during a given time interval. Ranking the days from the view-point of the stability of their radiative regime is performed by using the daily average value of the sunshine stability number and appropriately defined values of disorder and complexity, respectively. Measurements performed in the Romanian town of Timisoara (latitude 45°46?? N, longitude 21°25?? E and 85?m altitude above mean sea level) are used here. They refer to time series of global and diffuse solar irradiance recorded at 15-s time interval between sunrise and sunset during all the days in 2009. 相似文献
4.
Milatz Marius Hüsener Nicole Andò Edward Viggiani Gioacchino Grabe Jürgen 《Acta Geotechnica》2021,16(11):3573-3600
Acta Geotechnica - Gauging the mechanical effect of partial saturation in granular materials is experimentally challenging due to the very low suctions resulting from large pores. To this end, a... 相似文献
5.
6.
Mustafa Y. Kaya Guillaume Dupont‐Nivet Jean‐Noël Proust Pierrick Roperch Laurie Bougeois Niels Meijer Joost Frieling Chiara Fioroni Sevin
zkan Altner Ezgi Vardar Natasha Barbolini Marius Stoica Jovid Aminov Mehmut Mamtimin Guo Zhaojie 《Basin Research》2019,31(3):461-486
The proto‐Paratethys Sea covered a vast area extending from the Mediterranean Tethys to the Tarim Basin in western China during Cretaceous and early Paleogene. Climate modelling and proxy studies suggest that Asian aridification has been governed by westerly moisture modulated by fluctuations of the proto‐Paratethys Sea. Transgressive and regressive episodes of the proto‐Paratethys Sea have been previously recognized but their timing, extent and depositional environments remain poorly constrained. This hampers understanding of their driving mechanisms (tectonic and/or eustatic) and their contribution to Asian aridification. Here, we present a new chronostratigraphic framework based on biostratigraphy and magnetostratigraphy as well as a detailed palaeoenvironmental analysis for the Paleogene proto‐Paratethys Sea incursions in the Tajik and Tarim basins. This enables us to identify the major drivers of marine fluctuations and their potential consequences on Asian aridification. A major regional restriction event, marked by the exceptionally thick (≤ 400 m) shelf evaporites is assigned a Danian‐Selandian age (ca. 63–59 Ma) in the Aertashi Formation. This is followed by the largest recorded proto‐Paratethys Sea incursion with a transgression estimated as early Thanetian (ca. 59–57 Ma) and a regression within the Ypresian (ca. 53–52 Ma), both within the Qimugen Formation. The transgression of the next incursion in the Kalatar and Wulagen formations is now constrained as early Lutetian (ca. 47–46 Ma), whereas its regression in the Bashibulake Formation is constrained as late Lutetian (ca. 41 Ma) and is associated with a drastic increase in both tectonic subsidence and basin infilling. The age of the final and least pronounced sea incursion restricted to the westernmost margin of the Tarim Basin is assigned as Bartonian–Priabonian (ca. 39.7–36.7 Ma). We interpret the long‐term westward retreat of the proto‐Paratethys Sea starting at ca. 41 Ma to be associated with far‐field tectonic effects of the Indo‐Asia collision and Pamir/Tibetan plateau uplift. Short‐term eustatic sea level transgressions are superimposed on this long‐term regression and seem coeval with the transgression events in the other northern Peri‐Tethyan sedimentary provinces for the 1st and 2nd sea incursions. However, the 3rd sea incursion is interpreted as related to tectonism. The transgressive and regressive intervals of the proto‐Paratethys Sea correlate well with the reported humid and arid phases, respectively in the Qaidam and Xining basins, thus demonstrating the role of the proto‐Paratethys Sea as an important moisture source for the Asian interior and its regression as a contributor to Asian aridification. 相似文献
7.
Marius Todsen 《Journal of Hydrology》1973,20(4):311-326
The partial differential equation which governs the seepage of water in unsaturated and saturated porous media is solved numerically by a generalized Newton iteration technique for two models, one ditch drainage model and one earth dam model. For each model, which is two-dimensional, a few hypothetical soils with different moisture retention curves are considered. In both models only drainage from an initially saturated soil occurs; thus, the problem of hysteresis is avoided. The results of the computations are compared with those of corresponding saturated (pure groundwater) models; solutions obtained earlier by this author and others.
Computational instability phenomena appear when the slope of the retention curves is made steep, i.e., for poorly-graded soils. 相似文献
8.
Marius G. Floriancic Benjamin M. C. Fischer Peter Molnar James W. Kirchner Ilja H.J. van Meerveld 《水文研究》2019,33(22):2847-2866
Catchments consist of distinct landforms that affect the storage and release of subsurface water. Certain landforms may be the main contributors to streamflow during extended dry periods, and these may vary for different catchments in a given region. We present a unique dataset from snapshot field campaigns during low‐flow conditions in 11 catchments across Switzerland to illustrate this. The catchments differed in size (10 to 110 km2), varied from predominantly agricultural lowlands to Alpine areas, and covered a range of physical characteristics. During each snapshot campaign, we jointly measured streamflow and collected water samples for the analysis of major ions and stable water isotopes. For every sampling location (basin), we determined several landscape characteristics from national geo‐datasets, including drainage area, elevation, slope, flowpath length, dominant land use, and geological and geomorphological characteristics, such as the lithology and fraction of quaternary deposits. The results demonstrate very large spatial variability in specific low‐flow discharge and water chemistry: Neighboring sampling locations could differ significantly in their specific discharge, isotopic composition, and ion concentrations, indicating that different sources contribute to streamflow during extended dry periods. However, none of the landscape characteristics that we analysed could explain the spatial variability in specific discharge or streamwater chemistry in multiple catchments. This suggests that local features determine the spatial differences in discharge and water chemistry during low‐flow conditions and that this variability cannot be assessed a priori from available geodata and statistical relations to landscape characteristics. The results furthermore suggest that measurements at the catchment outlet during low‐flow conditions do not reflect the heterogeneity of the different source areas in the catchment that contribute to streamflow. 相似文献
9.
Subglacial emplacement of tills and meltwater deposits at the base of overdeepened bedrock troughs
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Marius W. Buechi Stephan M. Frank Hans Rudolf Graf John Menzies Flavio S. Anselmetti 《Sedimentology》2017,64(3):658-685
The sedimentary infills of subglacially eroded bedrock troughs in the Alps are underexplored archives for the timing, extent and character of Pleistocene glaciations but may contain excellent records of the Quaternary landscape evolution over several glacial–interglacial cycles. The onset of sedimentation in these bedrock troughs is often reflected by diamicts and gravels directly overlying bedrock in the deepest basin segments. Subglacial or proglacial depositional environments have been proposed for these coarse‐grained basal units but their characteristics and origin remain controversial. This article presents results from drill cores that recovered a coarse‐grained basal unit in a major buried bedrock‐trough system in the Lower Glatt Valley, northern Switzerland. The excellent core recovery allowed a detailed study combining macroscopic, microscopic and geochemical methods and gives unprecedented insights into the transition from erosion to deposition in overdeepened bedrock troughs. These results show that the basal infill comprises diamicts, interpreted as subglacial tills, separated by thin sorted interbeds, originating from subglacial cavity deposition. The stacking of these units is interpreted to represent repeated switching between a coupled and decoupled ice–bed‐interface indicating an ever‐transforming mosaic of subglacial bed conditions. Decoupling in response to high basal water pressures is probably promoted by the confined subglacial hydraulic conditions resulting from the bedrock acting as aquitards, the narrow reverse sloping outlet and a large catchment area. While stratigraphic and lithological evidence suggests that erosion and the onset of basal sedimentation occurred during the same glaciation, different scenarios for the relative timing of infilling in relation to formation and glaciation of the bedrock trough are discussed. Overlying deltaic and glaciolacustrine sediments suggest deposition during subsequent deglaciation of the bedrock trough. The basal sediment characteristics are in agreement with previous reports in hydrogeological and seismic exploration and suggest the occurrence of similar basal successions in other subglacially overdeepened basins in the Alps and elsewhere. 相似文献
10.
Motor vehicles historically have been dangerous locations to shelter in during tornado events. Throughout the twentieth century, motor vehicle design has become safer while tornado forecasting has become better understood. Despite such advances, tornado fatalities in motor vehicles still occur today, and some events periodically result in high numbers of deaths (e.g., ten motor vehicle occupants were killed by a single tornado in Garland, Texas, in 2015). We seek to examine all US tornado-induced motor vehicle fatalities documented between 1991 and 2015. Our findings indicate that motor vehicle fatalities have not significantly changed during this study period. We attribute annual fatality totals to persons lacking awareness of impending dangers coupled with numbers of significant tornado events for a given year. We find most fatalities result when vehicles are lofted or passengers are ejected, and this most typically occurs at the EF3–EF5 intensity thresholds. Fatalities that occur at weaker tornado winds (EF0–EF2) are most often attributed to collapsing debris (mostly trees) on vehicles. Spatially, motor vehicle fatalities are greatest in the Deep South and southern Great Plains regions where overall tornado and nighttime tornado frequencies are greatest. Some of the largest motor vehicle fatality events have resulted from tornadoes not being distinctly visible to motorists; such events have been characterized by tornadoes occurring at night or by tornadoes not appearing as “classic funnels.” 相似文献