Modelling the relationship between sexual reproduction and rhizome growth in Posidonia oceanica (L.) Delile

The relationship between flowering and growth performance of Posidonia oceanica (L.) Delile in meadows distributed along the south‐eastern coast of Sicily (Italy) was investigated by means of a statistical model (generalized linear mixed model) combined with the lepidochronological analysis. Over a 28‐year period, 67 floral stalk remains were observed. The highest flowering index was recorded in lepidochronological year 1998 (10.1%) and the Inflorescence Frequency per age showed a clear decrease corresponding to 15‐year‐old shoots. The sexual reproductive event had positive effects on rhizome elongation (cm year^?1) and leaf production (no. leaves year^?1) in the same flowering year, whilst no effect on the rhizome production (mg year^?1) was observed. Rhizome growth variables showed significant negative lagged responses in the two years following flowering. On the whole, we calculated that the effect exerted by flowering, in terms of loss on rhizome elongation and production, was about 27% and 38%, respectively. Although it has been demonstrated that recovery from the stress induced by sexual reproduction is limited to the two years after flowering, the magnitude of the reproductive cost may become quite considerable especially in comparison with the whole lifespan of individual shoots.     

Effects of tilling methods on soil penetration resistance,organic carbon and water stable aggregates in a vineyard of semiarid Mediterranean environment

Tillage, especially in semiarid Mediterranean environment, enhances the mineralization process of soil organic matter (SOM) and, in turn, decreases aggregate stability. Furthermore, continuous tillage leads to the formation of plough pan beneath the tilled layer. In the present study, we investigated the effect of an innovative self-propelled machine (spading machine, SM) for shallow tillage on SOM, water stable aggregates (WSA) and soil penetration resistance (PR). Such effects were compared to those of chisel plough (CP), rotary tiller (RT) and no tillage (NT). Each tilling method was applied up to a depth of 15 cm, whereas in NT only a brush cutter was used for weed control. Soil analyses were performed at the start of the experiment (March 2009, T0), in April 2010 (T1), May 2012 (T3), and June 2014 (T5) at both 0–15 and 15–30 cm. Compared to T0, soil PR increased with time in all the treatments and generally followed the order SM?<?RT?<?CP?<?NT. In soil tilled with the SM, soil PR never exceeded 2.5 MPa that was demonstrated to be a critical value for root elongation, and no evidence of the formation of plough pan beneath the tilled layer was observed. SOC as well as water content and WSA were higher in SM compared with CP and RT. In conclusion, the spading machine was proved to be more efficient in lowering the soil PR and in avoiding the formation of the plough pan. Furthermore, SM increased SOC and WSA.     

Crust and Upper Mantle Structure in the Caribbean Region by Group Velocity Tomography and Regionalization

An overview of the crust and upper mantle structure of Central America and the Caribbean region is presented as a result of the processing of more than 200 seismograms recorded by digital broadband stations from SSSN and GSN seismic networks. Group velocity dispersion curves are obtained in the period range from 10s to 40s by FTAN analysis of the fundamental mode of the Rayleigh waves; the error of these measurements varies from 0.06 and 0.09 km/s. From the dispersion curve, seven tomographic maps at different periods and with average spatial resolution of 500 km are obtained. Using the logical combinatorial classification techniques, eight main groups of dispersion curves are determined from the tomographic maps and eleven main regions, each one characterized by one kind of dispersion curves, are identified. The average dispersion curves obtained for each region are extended to 150s by adding data from a larger-scale tomographic study (Vdovin et al., 1999) and inverted using a nonlinear procedure. A set of models of the S-wave velocity vs. depth in the crust and upper mantle is found as a result of the inversion process. In six regions we identify a typically oceanic crust and upper mantle structure, while in the other two the models are consistent with the presence of a continental structure. Two regions, located over the major geological zones of the accretionary crust of the Caribbean region, are characterized by a peculiar crust and upper mantle structure, indicating the presence of lithospheric roots reaching, at least, about 200 km of depth.     

Effects of hydrocarbon contamination on a free living marine nematode community: results from microcosm experiments

Anthropogenic inputs of crude and refined petroleum hydrocarbons into the sea require knowledge of the effects of these contaminants on the receiving assemblages of organisms. A microcosm experiment was carried out to study the influence of diesel on a free living nematode community of a Tunisian lagoon. Sediments were contaminated by diesel that ranged in concentration from 0.5 to 20 mg diesel kg⁻¹ dry weight (dw), and effects were examined after 90 days. Gradual changes in community structure were revealed depending on the quantity of diesel administrated. In the medium (1 mg diesel kg⁻¹ and 5 mg diesel kg⁻¹ (dw)) and high (10 mg diesel kg⁻¹, 15 mg diesel kg⁻¹ and 20 mg kg⁻¹ (dw)) treated microcosms, most univariate measures, including diversity and species richness, decreased significantly with increasing level of diesel contamination whereas nematode assemblage from the low treated microcosm (0.5 mg diesel kg⁻¹ (dw)) remained unaffected. Results from multivariate analyses of the species abundance data demonstrated that responses of nematode species to the diesel treatments were varied: Chaetonema sp. was eliminated at all doses tested and seemed to be intolerant species to diesel contamination; Pomponema sp. and Oncholaimus campylocercoïdes were significantly affected at all diesel contamination levels but they were not eliminated, these species were categorized as “diesel-sensitive”; Hypodontolaimus colesi, Daptonema trabeculosum and Daptonema fallax which significantly increased respectively at 0.5, 1 and 5 mg diesel kg⁻¹ (dw) concentrations and appeared to be “opportunistic” species at these doses whereas Marylynnia stekhoveni which increased at all high doses (10, 15 and 20 mg diesel kg⁻¹ (dw)) seemed to be a “diesel-resistant” species.     

Mass Transfer Velocity and Momentum Vertical Exchange in Simulated Deep Street Canyons

A box model to simulate mass transfer inside deep street canyons and with atmospheric flow above is introduced and discussed. Two ideal deep street canyons with aspect ratios of 3 and 5 (the aspect ratio being the ratio between building height and street width H/W) are considered. This range of aspect ratios, found in many densely populated historical centres in Mediterranean cities as well as in other cities around the world, potentially creates high air pollutant concentration levels. Our model is based on a combination of analytical solutions and computation fluid dynamics (CFD) simulations using carbon monoxide (CO) as a tracer pollutant. The analytical part of the model is based on mass transfer velocity concepts while CFD simulations are used both for a preliminary validation of the physical hypothesis underlying the model (steady-state simulations) and to evaluate the concentration pattern with time (transient or wash-out simulations). Wash-out simulation curves were fitted by model curves, and mass transfer velocities were evaluated through a best-fitting procedure. Upon introducing into the model the contribution of traffic-produced turbulence, the modelled CO concentration levels became comparable with those obtained in real-world monitoring campaigns. The mass transfer rate between the canyon and the above atmosphere was then expressed in terms of an overall mass transfer velocity, which directly allows the evaluation of the mass transfer rate between the bottom volume of the canyon (pedestrian level) with the above atmosphere. Overall mass transfer velocities are reported as a function of the operating conditions studied (H/W = 3–5 and wind speeds = 2–8 ms⁻¹). Finally, a simple expression is reported for determining pollutant concentrations at the pedestrian level based on the overall mass transfer velocity defined.     

Natural background levels for some ions in groundwater of the Campania region (southern Italy)

The aim of the study was the determination of the natural background levels (NBLs) for the ions Na⁺, Cl^?, SO₄ ^2?, As³⁺, F^?, Fe²⁺, and Mn²⁺, in some groundwater bodies of the Campania region (southern Italy). The ??Protocol to evaluate the natural background concentrations?? proposed in 2009 by ISPRA (Italian Institute for Environmental Protection and Research) has been applied to the chemical data set of groundwater of the examined groundwater bodies. These analyses have also been examined following the guidelines of the BRIDGE project (Background cRiteria for the IDentification of Groundwater thrEshold). These approaches to evaluate the Threshold Values (TVs) and the NBLs, based on probability distribution functions, have been applied in many countries by various authors during the last 5?years. Changes applied to ISPRA Protocol in this study concern mainly the preselection criteria, in particular threshold values of specific ions, deriving from the aquifers geochemical features. The preselection criteria of the ISPRA Protocol have been merged with those of the BRIDGE Project in order to define a procedure suitable for the definition of the NBLs in the examined aquifers. The NBL of fluoride for the ??Phlegrean Fields?? and the ??eastern Plain of Naples?? groundwater bodies shows values deeply exceeding the reference value (REF) of 1,500???g/L, ranging between 3,600 and 15,000???g/L. The cause of this very high fluoride content is in the natural features of the aquifers constituted by volcanic and pyroclastic rocks. The volcanic origin of the aquifers is also the reason for the high arsenic content in ??Phlegrean Fields?? groundwater. Here the NBL calculated was about 47???g/L against the drinking water standard value of 10???g/L. The widespread high content of manganese and iron for the groundwater body of the ??eastern Plain of Naples?? is due to the reducing conditions related to the extensive marshlands present in the past. The very high NBL of all the examined ions for the groundwater body of ??Ischia Island?? depends on the existence of a geothermal system.     

Evaluation of microwave-assisted acid extraction procedures for the determination of metal content and potential bioavailability in sediments

Acid extractions represent a simple and effective tool for the assessment of metal contamination, origin and bioavailability in the aquatic environment. Optimized microwave-assisted procedures utilizing two extractions (8 M HNO₃ and 1 M HCl) were developed. Particular attention was given to the evaluation of efficiency and selectivity of the developed procedures. This involved an examination of the sediment and the extraction residues by Mössbauer spectroscopy and X-ray diffractometry. These instrumental approaches were used to identify the geochemical phases associated with acid decomposition. Microwave digestion with 8 M HNO₃ produced an efficient solubilization of the most important metal-bearing phases and left unaffected only the minerals (quartz, feldspars, muscovite) with a negligible metal content, thus providing a good estimate of the total metal concentrations. The 1 M HCl extraction, despite the limitation due to the incomplete solubilization of pyrite and to the partial dissolution of sheet silicates, represents a simple and effective technique for providing an estimate of the bioavailable metal fraction in sediments. The combined use of the two microwave-assisted acid extractions can provide a rapid and simple approach for the risk assessment of metal-polluted sediments.