海洋走航式单边连续观测地震折射波Δt解释方法--1种新的浅层折射单支时距曲线解释方法

在浅层工程折射地震测量中 ,通常采用相遇观测系统采集数据和相应的解释方法进行解释 ;对于单边观测采集方法由于与其相应的解释方法较少而少被采用。本文提出 1种方法简单、精度可靠、可直接利用同一点相邻 2排列 2次观测的初至折射时间并适合于海洋走航式单边连续观测系统的折射初至资料解释方法。其精度基本与同观测段的采用相遇观测的差异时距方法解释的精度十分接近。该方法即适合于人工解释又适合于计算机自动解释。     

Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE

Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.     

恶劣环境下GPS/BDS混合双差模糊度固定性能分析

针对在恶劣观测环境下每个GNSS系统只观测到较少的几颗卫星,采用标准双差模型很难实现整周模糊度固定的问题,该文对GPS/BDS系统间混合双差模型进行研究,利用该模型计算出GPS/BDS系统偏差。在此基础上,利用实验分别对模拟和真实恶劣观测环境下的模糊度固定性能进行分析,实验结果表明,恶劣环境下GPS/BDS系统间混合双差模型表现良好,相较于经典的双差模型可将模糊度固定平均所需时间分别缩短43%和33%,模糊度固定成功率分别提高107%和31%。     

Effects of Seawater Acidification on Early Development of Clam Cyclina sinensis

Anthropogenic emission of atmospheric carbon dioxide(CO_2) has led to a rapid increase in atmospheric CO_2 concentration. Increasing atmospheric CO_2 can reduce seawater pH and carbonate ions, which may adversely affect the survival of the larvae of calcareous animals. Cyclina sinensis is a commercially and ecologically important species in several Asian countries. Living in coast shallow waters, this species has experienced the coastal environmental changes frequently throughout its life cycle. In this study, we simulated possible future seawater pH values including 8.2, 7.8 and 7.4 and examined the effects of ocean acidification on the early development of C. sinensis. Clam embryos were incubated for 48 h(2 d) in control and high-CO_2 seawater to compare embryogenesis, larval growth and swimming behavior. Fertilization rate was quite sensitive to pH, and moderate acidification could induce a significant decrease in fertilization rate. However, only extreme acidification could bring significant negative effect to hatching rate, body size, and average path velocity of trochophora. Moreover, with seawater acidification, C. sinensis needs much more time to reach the same developmental stage, which increases the risk of larva survival. Together with recent studies demonstrating negative impacts of high CO_2 on fertilization and larva swimming behavior, the results imply a future decrease of C. sinensis populations in oceans if its acclimation to the predicted environmental alteration does not occur.     

水下光学无线通信的海水信道特性研究

提出了将具有比声学高达几倍的数据通信速率,良好的安全性和隐蔽性的光学无线通信技术应用于海军、海洋科学研究和水下工程等领域,实现高速率的水下无线通信技术实现海量数据的信息交换.基于生物光学特性的水下光学信道模型,建立了水下光学通信系统性能分析方法.并对基于发光二极管(LED)的水下光学无线通信系统进行了仿真,其结果表明所建立的方法可以进行各种海域水质环境的模拟,便于时水下光学无线通信系统性能进行预测评估,为水下光学无线通信系统的设计方案的评估提供了依据.     

红藻碳代谢的研究进展

红藻门(Rhodophyta)在全世界约有760属,4 410种,绝大多数海产,少数生于淡水,分布于各地,包括极地.中国已知有127属,300种,分布于南北各海区,淡水种类极少.     

地层失水沉降诱发井筒破裂治理效果的三维数值分析

运用三维有限差分软件FLAC3D对破裂井筒的各种治理措施进行了数值模拟,分析评价了钢圈加固和喷射混凝土套壁、壁后注浆、地面注浆、开设卸压槽等各治理措施的工程效果。结果表明,钢圈加固和喷射混凝土套壁强度相对较小,适合紧急的抢险处理和防止破裂混凝土坠落,难以维持井壁的长期稳定;破壁注浆措施在一定程度上控制了井壁与土层相对位移的增大,防治井筒破裂效果良好,且预防性壁后注浆效果更为突出;地面注浆防治效果明显,在注浆孔数量不变的条件下,孔位的合理选择对保证地面注浆防治效果至关重要;卸压槽可以有效吸收纵向变形以抑制破裂井壁的发展,其开设在破裂处上部时,效果最好。分析结果可用于指导破裂井筒治理的设计与施工     

碎石桩复合地基的抗液化特性探讨

饱和砂土地震液化问题是岩土地震工程中一个重要的研究课题。在多种可行的防治液化措施中 ,最普遍的方法是采用碎石桩复合地基。本文结合目前国内外碎石桩复合地基的抗液化研究的最新进展 ,对碎石桩的密实、排水减压和减震作用做了较详细的评述 ,最后还提出碎石桩复合地基抗液化特性需要进一步研究的问题     

潍坊市洪水资源利用现状及开发利用对策研究

洪水资源利用是解决潍坊市水资源短缺的重要途径.本文系统闸述了潍坊市降水及洪水特征,进行了洪水资源利用现状和潜力分析,提出了洪水资源安全利用的原则、前提条件、合理配置及利用模式,为该地区洪水资源的合理开发,持续利用提供了依据.