To analyze global gene expressions, we constructed a cDNA microarray from a basal chordate, the ascidian Ciona intestinalis. Ciona is a cosmopolitan species and a genomic analysis of Ciona revealed that ascidians had approximately 15,500 protein-coding genes. Our "Ciona intestinalis cDNA chip version 1 (Ci cDNA chip ver. 1)" has arrayed 13,400 unique Ciona cDNAs. To establish a detection system for gene expression profiles in wild ascidians using a cDNA microarray, we analyzed gene expressions in the whole body of Ciona adults after exposure to 100 nM tributyltin (TBT) for 24 h. In our preliminary array data using Ci cDNA chip ver. 1, we found more than 200 genes that showed strong differential expressions. These genes encoded proteins that were concerned with stress response, detoxification, oxidoreduction reaction, biosynthesis, and catabolism. This, the first large cDNA microarray of this animal, should facilitate analyses of global gene expressions following exposure to TBT. 相似文献
This paper briefly introduces the history of the study of the Pacific decadal oscillation (PDO) and explores the relationship between the PDO and sediment grain size from two typical sediment cores from the lower Changjiang (Yangtze River) and Huanghe (Yellow River) estuaries. It is found that the median grain sizes of both cores exhibit relatively high correlation with the PDO. This is because the PDO causes interdecadal variability of precipitation in the East Asia Monsoon region, thus changing the hydrodynamics in both the Changjiang and Huanghe catchments, eventually resulting in variation of sediment grain size. Our analysis also revealed that during different phases of the PDO, the sediment grain size of the Changjiang and Huanghe estuaries showed different variations in cold and warm PDO phases. This is related to movement of the precipitation center driven by the shift in different PDO phases. Moreover, we compared more high resolution geological proxies with the PDO, including stalagmites and tree rings, in East China over the past century. The results indicate that variations of studied geological proxies are generally well correlated with the PDO but have some differences. Finally, longer variations of sediment grain sizes in the Changjiang and Huanghe estuaries are compared with a reconstructed PDO over the last 200 years; sediment grain sizes were still correlated with the PDO, implying that sediment grain size may be used as a new proxy for studying the long-term behavior of the PDO. This result supports previous knowledge of the PDO impact on East China climate evolution and offers a new proxy for further PDO study. Our study will improve paleoenvironment reconstruction in East China on a decadal time scale and benefit future climatic predictions. 相似文献
In certain field conditions such as offshore projects under wave loads or embankments under traffic loads, both the vertical and horizontal stresses are variable. However, previous investigations rarely considered the variation in horizontal stress. To better understand the characteristics of natural saturated soft clay, a series of monotonic and cyclic triaxial tests with a K0-consolidation state were carried out under a variable confining pressure (VCP) stress path. The development of axial strain, pore water pressure and effective stress path is analysed. The results show that with the increase in η (the ratio of the variation in the mean effective principal stress to that of the deviatoric stress), the undrained shear strength (qf) decreases continuously. The pore water pressure generation is slightly improved under a stress path with increasing confining pressure. Based on the test results, a unified formula was established to predict the pore water pressure under VCP stress paths. The unique p–q–e relationship of normally consolidated clay in monotonic VCP triaxial tests was also demonstrated. Under VCP stress paths, the amplitude of the pore pressure increases, and the effective stress path tilts more sharply to the right. Moreover, a unified formula was established that can provide a good reference for predicting effective stress paths under cyclic VCP triaxial tests.
The surface accuracy of a large parabolic antenna is an important indicator to evaluate the quality of the antenna.It not only directly affects the antenna’s aperture efficiency,thereby determining the shortest wavelength that the antenna can work,but also affects the main lobe width and side lobe structure of the antenna pattern.Microwave holography is an important method for parabolic antenna profile detection.In this article we adopt a new algorithm to adjust the panels for the large radio telescope with an active main reflector through the TM65 m antenna’s aperture phase profile.The panels of the TM65 m radio telescope is in a radial pattern with 14 rings.Each corner of the panel is fixed on the screw of the actuator to move up and down,and the adjacent corners of the four panels share an actuator.We use the method of plane fitting to calculate the adjustment value of every panel’s corner.But one actuator,which simultaneously controls the common corner of the adjacent panels,will have different adjustment values according to the different plane fitting equation based on adjacent panels.In this paper,the adjustment value of the adjacent panels’crosspoints are constrained to be equal to the constraint condition to calculate each actuator’s adjustment value of the TM65 m radio telescope.Through multiple adjustments and application of the new algorithms,the accuracy of the TM65 m antenna reflector profile has been improved from the original 0.28 mm to the current 0.19 mm. 相似文献