Genetic diversity and population structure of the franciscana dolphin,Pontoporia blainvillei,in Southern Buenos Aires,Argentina

The franciscana dolphin, Pontoporia blainvillei, is endemic to the coastal waters of the southwestern Atlantic Ocean and the most endangered dolphin in the area. Four Franciscana Management Areas (FMAs) are currently recognised; however, results of genetic studies suggest the requirement for additional FMAs and highlight the need for more detailed studies in the southern extreme of the species distribution. With this aim, we studied the genetic diversity and population structure of the species analysing an mtDNA control region fragment (434?bp) in 44 individuals collected in four sampling sites located in Southern Buenos Aires. Haplotype diversity (H?=?0.75?±?0.05) was mostly higher than the observed in endangered or near threatened odontocetes. Population structure analyses suggest that three different genetic populations should be recognised within FMA IV: Northern, Eastern and Southern Buenos Aires. Altogether, these results should be taken into account in future conservation plans for the species.     

Development of airborne oil thickness measurements

A laboratory sensor has now been developed to measure the absolute thickness of oil on water slicks. This prototype oil slick thickness measurement system is known as the laser-ultrasonic remote sensing of oil thickness (LURSOT) sensor. This laser opto-acoustic sensor is the initial step in the ultimate goal of providing an airborne sensor with the ability to remotely measure oil-on-water slick thickness. The LURSOT sensor employs three lasers to produce and measure the time-of-flight of ultrasonic waves in oil and hence provide a direct measurement of oil slick thickness. The successful application of this technology to the measurement of oil slick thickness will benefit the scientific community as a whole by providing information about the dynamics of oil slick spreading and the spill responder by providing a measurement of the effectiveness of spill countermeasures such as dispersant application and in situ burning.

This paper will provide a review of early developments and discuss the current state-of-the-art in the field of oil slick thickness measurement.     

Studies of the formation process of water-in-oil emulsions

This paper summarizes studies to determine the formation process of water-in-oil emulsions and the stability of such emulsions formed in the laboratory and in a large test tank. These studies have confirmed that water-in-oil mixtures can be grouped into four states: stable emulsions, unstable water-in-oil mixtures, mesostable emulsions, and entrained water. These states are differentiated by rheological properties as well as by differences in visual appearance. The viscosity of a stable emulsion at a shear rate of one reciprocal second is about three orders of magnitude greater than that of the starting oil. An unstable emulsion usually has a viscosity no more than about 20 times greater than that of the starting oil. A stable emulsion has a significant elasticity, whereas an unstable emulsion does not. A mesostable emulsion has properties between stable and unstable, but breaks down within a few days of standing. The usual situation is that emulsions are either obviously stable, mesostable, or unstable. Entrained water, water suspended in oil by viscous forces alone, is also evident. Very few emulsions have questionable stability. Analytical techniques were developed to test these observations.

The type of emulsion produced is determined primarily by the properties of the starting oil. The most important of these properties are the asphaltene and resin content and the viscosity of the oil. The composition and property ranges of the starting oil that would be required to form each of the water-in-oil states are discussed in this paper.     

Studies on water-in-oil products from crude oils and petroleum products

Water-in-oil mixtures such as emulsions, often form and complicate oil spill countermeasures. The formation of water-in-oil mixtures was studied using more than 300 crude oils and petroleum products. Water-in-oil types were characterized by resolution of water at 1 and 7 days, and some after 1 year. Rheology measurements were carried out at the same intervals. The objective of this laboratory study was to characterize the formed water-in-oil products and relate these properties to starting oil properties. Analysis of the starting oil properties of these water-in-oil types shows that the existence of each type relates to the starting oil viscosity and its asphaltene and resin contents. This confirms that water-in-oil emulsification is a result of physical stabilization by oil viscosity and chemical stabilization by asphaltenes and resins. This stabilization is illustrated using simple graphical techniques. Four water-in-oil types exist: stable, unstable, meso-stable and entrained. Each of these has distinct physical properties.     

Review of behaviour of oil in freezing environments

The current knowledge of the physical fate and behaviour of crude oil and petroleum products spilled in Arctic situations is reviewed. The fate and final deposition of oil in marine conditions is presented as based on the extant literature.

Spreading models were evaluated for oil on ice, under ice, in snow, in brash ice, and between blocks of ice. Models of oil transport under sheet and broken ice were considered, both for sea and river conditions. The ability of ice sheets to trap oil is discussed in relation to oil storage capacity. The effects of oil on a growing ice sheet were examined, both in terms of ice formation and the thermal effects of oil inclusions in ice. The migration of oil through ice was reviewed, focussing primarily on the movement through brine channels. The effects of oil on the surface of ice were considered, with emphasis on the effects of surface pools on ice melt. Similar consideration was given to the effects of oil on snow on the surface of ice.

The few quantitative studies of oil in open and dynamic ice conditions are reviewed. Observations of intentional small-scale spills in leads and ice fields are reviewed and compared with observations from real spills. The conditions under which “oil pumping” from leads occurs were quantified. The most common ultimate fate of oil in an ice field is to be released onto the water surface.     

Review of the development of laser fluorosensors for oil spill application

As laser fluorosensors provide their own source of excitation, they are known as active sensors. Being active sensors, laser fluorosensors can be employed around the clock, in daylight or in total darkness. Certain compounds, such as aromatic hydrocarbons, present in petroleum oils absorb ultraviolet laser light and become electronically excited. This excitation is quickly removed by the process of fluorescence emission, primarily in the visible region of the spectrum. By careful choice of the excitation laser wavelength and range-gated detection at selected emission wavelengths, petroleum oils can be detected and classified into three broad categories: light refined, crude or heavy refined.

This paper will review the development of laser fluorosensors for oil spill application, with emphasis on system components such as excitation laser source, and detection schemes that allow these unique sensors to be employed for the detection and classification of petroleum oils. There have been a number of laser fluorosensors developed in recent years, many of which are strictly research and development tools. Certain of these fluorosensors have been ship-borne instruments that have been mounted in aircraft for the occasional airborne mission. Other systems are mounted permanently on aircraft for use in either surveillance or spill response roles.     

Astronomical frequencies for climate research at the decadal to century time scale

Short-term variations of the elements representing the Earth's motion around the Sun and its rotation have been analyzed over the last 6000 years using 1-year steps. Their low-frequency part is compared first to the values obtained from a secular theory of the planetary long-term motion showing that they can be considered reliable enough to represent adequately the motion of the Earth over the last 5000 years. Spectral analysis of these values shows that the main periodicities are 2.67, 3.98, 5.26, 5.93, 7.9, 9.8, 11.9, 14.7, 15.8, 29, 42, 61, 122, 165 and 250 years for the eccentricity as well as for the climatic precession, with an additional component at around 930 years for the eccentricity and around 840 years for the climatic precession. Periodicities at 2.67, 3.8, 5.9, 8.0, 9.3, 11.9, 14.7, 18.6, 29, 135, 250 and 840 yr are also shown for the obliquity. Spectral analyses of the daily July mid-month insolation at 65°N show essentially the same periodicities as the climatic precession and the obliquity, i.e. 2.67, 3.98, 5.92, 8.1, 11.9, 15.7, 18.6, 29, 40, 61 and around 900 years. Finally a wider analysis of the insolation pattern was performed related to the large periodicity band of the insolation time series for the solstices and the equinoxes for 7 different latitudes. In equatorial latitudes the insolation variance is largely explained by precession. But precession dominates everywhere with the obliquity signal being stronger at polar latitudes at the solstices. The amplitudes of the insolation change at these frequencies is of the order of 0.2 Wm^–2 at the maximum. Offprint requests to: A Berger     

西藏丁青弧前蛇绿岩的地球化学特征

丁青蛇绿岩位于班化湖－丁青－怒江蛇绿岩带的东段，其地幔岩出露规模是该带中最大的。本文报道的丁青蛇绿岩主要由地幔橄榄岩、堆晶岩、辉长岩和斜长花岗岩组成。蛇绿岩剖面上覆硅岩中的放射虫化石是早株罗世和晚三叠世诺利克期的，中侏罗统砂岩和砾岩不整合覆盖在蛇绿岩之上，由此确定丁青蛇绿岩是晚三叠－早侏罗世的，在中株罗世之前侵位，丁青蛇绿岩属于玻安岩系，玻安岩的特点是富Si、Mg和大离子亲石元素（LILE），贫高场强元素（Ti、P、Zr、Y、Yb和Nb）。丁青蛇绿岩的堆晶岩、辉长岩和辉绿岩均具“U”型REE分布，暗示丁青玻安岩是由于亏损的地幔源岩和来自消减带的水和流体两组分的混合形成的。丁青玻安岩的地球化学特征类似西太平洋第三纪玻安岩，而明显不同于MORB的地球化学性质， 表明丁青玻安岩应当形成于洋内岛弧的弧前环境，属于弧前蛇绿岩。     

Development of oil hydrocarbon fingerprinting and identification techniques

Oil, refined product, and pyrogenic hydrocarbons are the most frequently discovered contaminants in the environment. To effectively determine the fate of spilled oil in the environment and to successfully identify source(s) of spilled oil and petroleum products is, therefore, extremely important in many oil-related environmental studies and liability cases. This article briefly reviews the recent development of chemical analysis methodologies which are most frequently used in oil spill characterization and identification studies and environmental forensic investigations. The fingerprinting and data interpretation techniques discussed include oil spill identification protocol, tiered analytical approach, generic features and chemical composition of oils, effects of weathering on hydrocarbon fingerprinting, recognition of distribution patterns of petroleum hydrocarbons, oil type screening and differentiation, analysis of “source-specific marker” compounds, determination of diagnostic ratios of specific oil constituents, stable isotopic analysis, application of various statistical and numerical analysis tools, and application of other analytical techniques. The issue of how biogenic and pyrogenic hydrocarbons are distinguished from petrogenic hydrocarbons is also addressed.