夜光藻的室内培养

夜光藻的室内培养对于研究夜光藻的生理生态特性及其增殖机理是一个关键性的环节。通过试验，发现在实验室条件下以扁藻作为饵料，夜光藻能良好的生长和繁殖，从而为夜光藻的室内培养解决了关键性的饵料问题，实现了国内夜光藻室内的长期培养。     

桥梁水平位移混沌特征识别与神经网络预测研究

跨海大桥系统受外界影响扰动,其变形伴有混沌现象发生.对桥梁变形监测数据实现了混沌识别,运用C-C法计算时间序列的延迟时间,用G-P方法求得最佳嵌入维数,通过求取的时间延迟和最佳嵌入维数对桥梁变形监测数据进行相空间重构,为混沌时间序列预测模型的建立奠定基础;基于RBF神经网络建立混沌时间序列预测模型,对实测数据进行桥梁变...     

二郎山公路隧道轴线地应力测试研究

介绍了二郎山公路隧道轴线上3个钻孔的地应力测试结果,应用水压致裂法在钻孔中测试,所测的两个不同大小的水平主应力都大于垂直主应力（除了地表以下很浅处外）,这个地应力与区域大地构造方向一致,在此基础上评价了隧道碉室围岩的稳定性。     

Analysis of phospholipids in microalga Nitzschia closterium by UPLC-Q-TOF-MS

Precise structural identification of phospholipids in the microalga Nitzschia closterium has been established using ultra performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (UPLC-ESI-Q-TOF-MS) for direct analysis of total lipid extracts. Mass spectrometry was performed in reflective time-of-flight using electron spraying ionization in negative mode. Phospholipid molecular species identification was based on the characteristic product ions and neutral loss yielded by different phospholipids under ESI-MS/MS mode. The molecular species were confirmed by the carboxylate anions produced by phospholipids in negative mode; the regiospecificity of the two acyl chains was determined from the ratio of sn-1 to sn-2 carboxylate anion abundances. As a result, 18 lipid molecular species were identified for the first time in this microalga, comprising seven phosphatidylcholines (PC), two phosphatidylethanolamines (PE), two phosphatidylinositols (PI), and seven phosphatidylglycerols (PG). Lipid standards of PC, PE, PI, and PG were added to the total lipids as internal standards for semiquantitative analysis, revealing concentrations of phospholipids in this species between 0.09 and 3.37 nmol/mg. This method can produce a full structural profile of intact phospholipid molecular species and can be used for study of the physiological and ecological functions of lipids by monitoring their individual changes over time.     

富营养化湖泊蓝藻—芦苇碎屑堆积分解过程及其潜在的共代谢效应

为探究富营养化浅水湖泊所富集的有机物对湖泊碳循环和水质的影响,本研究构建微宇宙系统,模拟蓝藻和芦苇碎屑单独分解及混合分解过程.通过测定各组上覆水营养盐浓度、有机质含量及结构的变化,揭示富营养化湖泊藻草残体混合分解过程中养分和有机碳的释放特征.结果 表明,在实验0～88 h内,在添加相同的碳源条件下,蓝藻和芦苇混合处理组...     

底板突水的数值计算方法研究

应用数值计算方法，分析了采动对底板隔水层岩体上都和下部的影响与破坏，对底板突水机理提出了一些规律性的认识。     

Comparative study of brine shrimp bioassay-based toxic activities of three harmful microalgal species that frequently blooming in aquaculture ponds

Journal of Oceanology and Limnology - Typical harmful micro-algal species constantly occurred in high density in marine aquaculture ponds in Xiangshan and Sanmen Bay, Zhejiang Province. Fates of...     

MapGIS平台下图件图式的定制与自动生成

通过分析MapGIS制图软件的工作特点,在微软.NET框架下应用C#语言和MapGIS K9(以下简称K9)的二次开发接口,在数据字典技术的支持下,总结了一套MapGIS平台下标准图式按需定制及自动生成的技术流程,对各行业的标准化制图及提高制图人员的工作效率有较好的作用,同时对相关人员研究其它数字化制图软件下的标准出图提供了一定的参考价值.     

Development and evaluation of a DNA microarray assay for the simultaneous detection of nine harmful algal species in ship ballast and seaport waters

Rapid, high-throughput and reliable methods are urgently required to accurately detect and monitor harmful algae, which are responsible for algal blooms, such as red and green tides. In this study, we successfully developed a multiplex PCR-based DNA microarray method capable of detecting nine harmful algal species simultaneously, namely Alexandrium tamarense, Gyrodinium instriatum, Heterosigma akashiwo, Karenia mikimotoi, Prorocentrum donghaiense, Prorocentrum minimum, Ulva compressa, Ulva ohnoi and Ulva prolifera. This method achieved a limit of detection (LOD) of 0.5 ng of genomic DNA (orders of magnitude of the deci-nanogram range) in the tested algae cultures. Altogether, 230 field samples from ship ballast waters and seaport waters were used to evaluate the DNA microarray. The clinical sensitivity and specificity of the DNA microarray assay in detecting field samples were 96.4% and 90.9%, respectively, relative to conventional morphological methods. This indicated that this high-throughput, automatic, and specific method is well suited for the detection of algae in water samples.     

山东平邑盆地第三纪卞桥组孢粉化石组合及地层意义

本文采集的孢粉化石来自山东平邑卞桥组。文中讨论了孢粉化石组合及其组合特征。该组合的地质时代为中、晚古新世。古气候为亚热带型。