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The aim of this study was to investigate the potential application of the beta-d-glucuronidase (GLUase) activity measurement for the routine detection and quantification of E. coli in marine bathing waters. GLUase activity was measured as the rate of hydrolysis of 4-methylumbelliferyl-beta-d-glucuronide. Culturable E. coli were quantified by the most probable number (MPN) microplate method. Both methods were applied to a large set of seawater samples. Significant correlation was found between the log of GLUase activity and the log of culturable E. coli. The mean coefficient of variation (CV) of the GLUase activity was less than 15% at concentrations around the current standards of International regulations whereas the CV of the microplate method was around 30%. When samples were stored at 4 degrees C and 20 degrees C, the mean CV of the GLUase activity remained below 15% up to 6 hours after sample collection whereas the range of variation of the microplate method varied between 10 and 50%. We concluded that the GLUase activity is an operational, reproducible, simple, very rapid and low cost method for the real-time enumeration of E. coli in bathing waters and should be preferred to the microplate method. The GLUase activity method should be routinely applied to the rapid enumeration of E. coli in recreational waters and recommendations for its application were suggested to water quality managers. 相似文献
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本文对构造地震和塌陷地震进行了对比分析,找出了它们的特征。指出了构造地震的波速大于塌陷地震的波速;构造地震波的视出射角大于塌陷地震的视出射角;塌陷地震S波最大振幅随距离的衰减比构造地震快。求出了构造地震尾波衰减系数 相似文献
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地震序列较强余震灰色及最小二乘拟合预测方法的应用研究 总被引:1,自引:0,他引:1
在收集、整理1966年~2002年8月我国(主要为大陆地区)183个5级以上地震序列资料的基础上,应用灰色预测和最小二乘拟合预测方法,对每一地震序列较强(显著)余震发震时间进行“硬性”检验预测。结果表明,2种预测方法对7级以上地震序列和6级地震序列的较强(显著)余震具有较好的预测效能,预测效能分别为76.2%和64.7%;而对5级地震序列预测效果则不甚明显。因此,灰色预测和最小二乘拟合预测方法主要着眼于对大(强)地震的较强(显著)余震预测,可望在地震现场大(强)震应急工作中发挥积极作用。 相似文献
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Santos RG Martins AS Farias Jda N Horta PA Pinheiro HT Torezani E Baptistotte C Seminoff JA Balazs GH Work TM 《Marine pollution bulletin》2011,62(6):1297-1302
To show the influence of coastal habitat degradation on the availability of food for green turtles (Chelonia mydas), we assessed the dietary preferences and macroalgae community at a feeding area in a highly urbanized region. The area showed low species richness and was classified as degraded. We examined stomach contents of 15 dead stranded turtles (CCL = 44.0 cm (SD 6.7 cm)). The diet was composed primarily of green algae Ulva spp. (83.6%). In contrast, the macroalgae community was dominated by the green alga Caulerpa mexicana. We found a selection for red algae, seagrass and Ulva spp., and avoidance for C. mexicana and brown alga Dictyopteris delicatula. The low diversity of available food items, possibly a result of environmental degradation, likely contributed to the low dietary diversity. The nutritional implications of this restricted diet are unclear. 相似文献
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华北地区地震活动幕的灰色预测 总被引:2,自引:0,他引:2
华北地区是我国地震活动的主要场所之一,历史上曾经发生过多次强震乃至大震,特别是本世纪以来发生在人口稠密地区的邢台、唐山两次地震,给人民生命财产造成了巨大损失,为此研究这一地区的未来地震活动趋势就显得尤为重要。 文献[1]从区域性地震群体活动的韵律性、分幕性及环境因子对地震发生的作用入手,研究了华北地震总趋势,认为:目前华北地区仍处于1978年以来的地震平静时段;报告的多年研究资料也都指 相似文献
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华北地区地震异常前兆信息场演化特征及其与地震关系的研究 总被引:6,自引:1,他引:6
在对地震异常前兆信息量先前研究的基础上,进一步给出了描述各种地震异常前兆信息量的普适性综合数学表达式.从大量震例资料入手,再次研究了地震异常延续有效性的问题,进而建立了地震异常持续有效时间与延续有效时间的数学关系式.收集整理了1980~1997年华北地区水化、形变、地磁、应力、水位、地电等371个观测项目、上百万个前兆观测数据,对每一观测项目逐一计算了它们的地震异常前兆信息量.研究了华北地区近20年的地震异常前兆信息场的演化特征及其与地震的关系,建立了华北地区Ms≥5地震的中短期综合预报指标体系,经R值评分检验,表明该地震综合预报指标体系在中短期地震预测中具有一定效果. 相似文献
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