A cDNA microarray technique applied for analysis of global gene expression profiles in tributyltin-exposed ascidians

To analyze global gene expressions, we constructed a cDNA microarray from a basal chordate, the ascidian Ciona intestinalis. Ciona is a cosmopolitan species and a genomic analysis of Ciona revealed that ascidians had approximately 15,500 protein-coding genes. Our "Ciona intestinalis cDNA chip version 1 (Ci cDNA chip ver. 1)" has arrayed 13,400 unique Ciona cDNAs. To establish a detection system for gene expression profiles in wild ascidians using a cDNA microarray, we analyzed gene expressions in the whole body of Ciona adults after exposure to 100 nM tributyltin (TBT) for 24 h. In our preliminary array data using Ci cDNA chip ver. 1, we found more than 200 genes that showed strong differential expressions. These genes encoded proteins that were concerned with stress response, detoxification, oxidoreduction reaction, biosynthesis, and catabolism. This, the first large cDNA microarray of this animal, should facilitate analyses of global gene expressions following exposure to TBT.     

Scaling model experiments on propagation of electromagnetic waves using salt solution to simulate the Earth's crust: Loma Prieta and Kobe earthquakes

Propagation of electromagnetic (EM) waves from an earthquake focus in the conductive Earth has been investigated using 1/1,000,000 scaling models taking earth-ionosphere and ocean-Moho plane parallel-plate waveguides into account. Microwaves at a frequency, ω_m, a million times higher than that of seismic EM signal (SEMS), ω, were generated at the model focus. They are propagated in a salt solution modeling the earth's crust and reflected by ocean, fault planes, ionosphere and Moho plane all made by aluminum. Distribution of EM power was mapped by scanning a detector antenna over the model Earth's surface. The skin depth, δ, calculated by the exact skin depth equation, 1/δ=ω(μ/2)^1/2 [(1+(1/ωρ)²)^1/2 −1]^1/2 where dielectric constant, and permeability, μ are the same but resistivity, ρ, 10⁻⁶ times smaller than that of Earth, gave 10⁻⁶ times small skin depth validating the model scaling index. Images for evanescent and wave-ripple standing waves disturbed by normal, strike-slip and dip-slip conductive fault planes have been obtained using an aluminum plate. The co-circular contour map above the epicenter due to evanescence was pushed to the north east direction from the epicenter by the presence of ocean for the Loma Prieta earthquake, while to north direction for the Kobe earthquake. The intensity of EM ULF emissions for the Loma Prieta earthquake is discussed quantitatively.     

Tropical synoptic-scale wave disturbances over the western Pacific simulated by a global cloud-system resolving model

Lower-tropospheric tropical synoptic-scale disturbances (TSDs) are associated with severe weather systems in the Asian Monsoon region. Therefore, exact prediction of the development and behavior of TSDs using atmospheric general circulation models is expected to improve weather forecasting for this region. Recent state-of-the art global cloud-system resolving modeling approaches using a Nonhydrostatic Icosahedral Atmospheric Model (NICAM) may improve representation of TSDs. This study evaluates TSDs over the western Pacific in output from an Atmospheric Model Intercomparison Project (AMIP)-like control experiment using NICAM. Data analysis compared the simulated and observed fields. NICAM successfully simulates the average activity, three-dimensional structures, and characteristics of the TSDs during the Northern summer. The variance statistics and spectral analysis showed that the average activity of the simulated TSDs over the western Pacific during Northern summer broadly captures that of observations. The composite analysis revealed that the structures of the simulated TSDs resemble the observed TSDs to a large degree. The simulated TSDs exhibited a typical southeast- to northwest-oriented wave-train pattern that propagates northwestward from near the equator around 150 ^° E toward the southern coast of China. However, the location of the simulated wave train and wave activity center was displaced northward by approximately a few degrees of latitude from that in the observation. This displacement can be attributed to the structure and strength of the background basic flow in the simulated fields. Better representation of the background basic states is required for more successful simulation of TSDs.     

The formation of large mudwaves by turbidity currents on the levees of the Toyama deep-sea channel, Japan Sea

The development of mudwaves on the levees of the modern Toyama deep‐sea channel has been studied using gravity core samples combined with 3·5‐kHz echosounder data and airgun seismic reflection profiles. The mudwaves have developed on the overbank flanks of a clockwise bend of the channel in the Yamato Basin, Japan Sea, and the mudwave field covers an area of 4000 km². Mudwave lengths range from 0·2 to 3·6 km and heights vary from 2 to 44 m, and the pattern of mudwave aggradation indicates an upslope migration direction. Sediment cores show that the mudwaves consist of an alternation of fine‐grained turbidites and hemipelagites whereas contourites are absent. Core samples demonstrate that the sedimentation rate ranged from 10 to 14 cm ka^?1 on the lee sides to 17–40 cm ka^?1 on the stoss sides. A layer‐by‐layer correlation of the deposits across the mudwaves shows that the individual turbidite beds are up to 20 times thicker on the stoss side than on the lee side, whereas hemipelagite thicknesses are uniform. This differential accretion of turbidites is thought to have resulted in the pattern of upcurrent climbing mudwave crests, which supports the notion that the mudwaves have been formed by spillover turbidity currents. The mudwaves are interpreted to have been instigated by pre‐existing large sand dunes that are up to 30 m thick and were created by high‐velocity (10°ms^?1), thick (c. 500 m) turbidity currents spilling over the channel banks at the time of the maximum uplift of the Northern Japan Alps during the latest Pliocene to Early Pleistocene. Draping of the dunes by the subsequent, lower‐velocity (10^?1ms^?1), mud‐laden turbidity currents is thought to have resulted in the formation of the accretionary mudwaves and the pattern of upflow climbing. The dune stoss slopes are argued to have acted as obstacles to the flow, causing localized loss of flow strength and leading to differential draping by the muddy turbidites, with greater accretion occurring on the stoss side than on the lee slope. The two overbank flanks of the clockwise channel bend show some interesting differences in mudwave development. The mudwaves have a mean height of 9·8 m on the outer‐bank levee and 6·2 m on the inner bank. The turbidites accreted on the stoss sides of the mudwaves are 4–6 times thicker on the outer‐bank levee than their counterparts on the inner‐bank levee. These differences are attributed to the greater flow volume (thickness) and sediment flux of the outer‐bank spillover flow due to the more intense stripping of the turbidity currents at the outer bank of the channel bend. Differential development of mudwave fields may therefore be a useful indicator in the reconstruction of deep‐sea channels and their flow hydraulics.     

The Meteoritical Bulletin,No. 111

Meteoritical Bulletin 111 contains the 3094 meteorites approved by the Nomenclature Committee of the Meteoritical Society in 2022. It includes 11 falls (Antonin, Botohilitano, Cranfield, Golden, Great Salt Lake, Longde, Msied, Ponggo, Qiquanhu, Tiglit, Traspena), with 2533 ordinary chondrites, 165 HED, 123 carbonaceous chondrites (including 4 ungrouped), 82 lunar meteorites, 28 Rumuruti chondrites, 27 iron meteorites, 23 ureilites, 22 mesosiderites, 22 Martian meteorites, 21 primitive achondrites (one ungrouped), 17 ungrouped achondrites, 13 pallasites, 7 enstatite achondrites, 6 enstatite chondrites, and 5 angrites. Of the meteorites classified in 2022, 1787 were from Antarctica, 1078 from Africa, 180 from South America, 34 from Asia, 6 from North America, 4 from Europe, and 1 from Oceania.     

Dissolution of silica accompanied by oxygen consumption in the bottom layer of Japan’s central Seto Inland Sea in summer

Distribution of silicic acid (Si(OH)₄) in bottom water was investigated in the central Seto Inland Sea under stratified conditions in summer. Water samples were collected at 10 stations on April 24 and 25 and July 7 and 8, 2012. In July, stratification progressed, and a cold water mass (dome) of <20 °C appeared. In response to the cold dome, low oxygen content was observed in the bottom layer of the eastern part of Hiuchi-Nada. In this water mass site, apparent oxygen utilization values calculated from dissolved oxygen (DO) concentrations increased, coinciding with increase of Si(OH)₄ concentrations from April to July. This suggests that increase of Si(OH)₄ [dissolution of biogenic silica (diatom frustules)] was accompanied by DO consumption due to degradation of organic matter such as plankton soft tissue. These findings suggest that a bacterial degradation of the organic matrix that covers diatom frustules could accelerate the dissolution of biogenic silica in bottom water under stratified conditions.     

A comparison of neighbourhood relations based on ordinary Delaunay diagrams and area Delaunay diagrams: an application to define the neighbourhood relations of buildings

ABSTRACT

The aim of this article is to describe a convenient but robust method for defining neighbourhood relations among buildings based on ordinary Delaunay diagrams (ODDs) and area Delaunay diagrams (ADDs). ODDs and ADDs are defined as a set of edges connecting the generators of adjacent ordinary Voronoi cells (points representing centroids of building polygons) and a set of edges connecting two centroids of building polygons, which are the generators of adjacent area Voronoi cells, respectively. Although ADDs are more robust than ODDs, computation time of ODDs is shorter than that of ADDs (the order of their computation time complexity is O(nlogn)). If ODDs can approximate ADDs with a certain degree of accuracy, the former can be used as an alternative. Therefore, we computed the ratio of the number of ADD edges to that of ODD edges overlapping ADDs at building and regional scales. The results indicate that: (1) for approximately 60% of all buildings, ODDs can exactly overlap ADDs with extra ODD edges; (2) at a regional scale, ODDs can overlap approximately 90% of ADDs with 10% extra ODD edges; and (3) focusing on judging errors, although ADDs are more accurate than ODDs, the difference is only approximately 1%.     

Effect of ocean acidification on coastal phytoplankton composition and accompanying organic nitrogen production

The effect of ocean acidification, caused by the increase in pCO₂ in seawater, on phytoplankton population and on related organic nitrogen production was experimentally examined by use of a natural coastal microbial population. pCO₂ and pH were controlled by aeration with air in which pCO₂ was at the current level (control), for which ambient air was used, and with air in which pCO₂ was ??800?? and ??1200?? ppm, in 500-L culture vessels. The experiment was continued for 15?days after addition of the inorganic nutrients such as nitrate, phosphate, and silicate. During most of the experimental period, a minor increase in phytoplankton biomass was noted, probably because of low irradiance, an increase in phytoplankton biomass was observed at the end of the experiment. Flow cytometric and microscopic observations revealed that this increase was because of Chrysochromulina sp. (Haptophyceae). The growth of Chrysochromulina sp. was most obvious in the control vessel, and tended to be obscured by increasing pCO₂ (decrease in pH), indicating the possibility that ocean acidification inhibits the growth of specific phytoplankton groups, for example Chrysochromulina sp. Production of particulate organic nitrogen (PON), determined by the ¹⁵N tracer method, also diminished under acidified conditions compared with that at the current level.     

Problems concerning the Directivity of Tsunami

If knowledge of our theories on the directivity of tsunamis had received worldwide attention, the following operations could have been carried out internationally just after the large earthquake of 19 September 1985 which occurred near Acapulco, Mexico. Having found the great circle, “line S” which is perpendicular to the coast around Acapulco, we could have calculated the angles between line S and line A and between line S and line D, where line A and line D are the great circle connecting Acapulco and Auckland, New Zealand and that connecting Acapulco and Duke of York Island (Chile), respectively. The resultant angles are 30?43′ and 41?49′(>68?48′/2), we could thereafter neglect the eastern half of the offshore energy flux. When we assume that the speed of trans-Pacific tsunami is 400 knots, the probability that the actual tsunami will come earlier than the calculated arrival time proves to be $$\frac{1}{{\sqrt {2\pi } }}\int_{ - {\text{ }}\infty }^{ - {\text{ }}0.689} {e^{ - t^{{2 \mathord{\left/ {\vphantom {2 2}} \right. \kern-\nulldelimiterspace} 2}} } dt = 0.2454} $$ Contact with New Zealand prior to the forecasted arrival time was essential, but the tsunami attention for the Japanese coast was unnecessary. Without such application of our directivity theories, frequent fruitless warnings will be issued for future trans-Pacific tsunamis. Quick improvements in warning procedures are required.