An evaluation of metal bioaccessibility in estuarine sediments using the commercially available protein, bovine serum albumin

The bioaccessibility of metals (Al, Ca, Fe, Mn, Ag, Cd, Co, Cu, Ni, Pb, Sn, Zn) in oxic estuarine sediments has been evaluated using solutions of a commercially available protein (bovine serum albumin; BSA) that mimic the chemical conditions encountered in the gut environment of many deposit-feeding organisms. Over a 20 h incubation period with 5 g L^− 1 BSA, metal mobilisation was generally biphasic in that a relatively short period of rapid release was succeeded by more gradual release or approach to equilibrium, although in some cases metal readsorption was evident during the time-courses. Availability to BSA, defined as metal released after 20 h relative to metal extracted by boiling aqua regia, was greatest for Cd, Ni, Ca and Zn and lowest for Fe and Mn, and correlated well with, but was an order of magnitude lower than, metal digested by acidified hydrogen peroxide. Time-courses conducted in the absence of the protein revealed that significant quantities of Ca and Mn were water-soluble, reflecting the partial dissolution of carbonates and hydrous Mn oxides, respectively. Additional experiments indicated a net increase in metal release with increasing BSA concentration and, with the exception of Ca and Mn, a substantial increase in metal mobilisation after sediment organic matter had been digested by peroxidation. These observations suggest that, apart from Ca and Mn, metal release proceeds via complexation with component amino acids of the protein, denudation of organic host phases, and exposure of inorganic, metal-bearing minerals. Accordingly, the bioaccessibility of a metal is predicted to be dependent on its (i) affinity for proteinaceous ligands, (ii) association with components of digestible sediment organic matter, and (iii) degree of binding at relatively weak sites on sediment phases that are exposed or modified by the action of proteins and other chemical constituents of the gut environment.     

Is delta-aminolevulinic acid dehydratase activity in bivalves from south-west Iberian Peninsula a good biomarker of lead exposure?

The coast of Huelva is considered as a heavily contaminated area where the Tinto and Odiel rivers discharge after running through a metalliferous mining area in the Iberian Pyrite Belt and end in common estuary called Ría of Huelva. Lead is a highly toxic and widely distributed element in the aquatic environment; therefore there is a great interest in assessing the impact of this contaminant on aquatic organisms. To study the bioavailability and sources of lead, the bivalve species Chamelea gallina was collected from six sampling sites along the coast of Huelva. Besides the effect of this metal on the enzyme activity, delta-aminolevulinic acid dehydratase (ALA-D) was determined in the whole tissue. Results show the inverse relationship between the lead concentration and the activity of ALA-D measured in whole tissues (r2=0.7). Individuals collected from the Ría of Huelva estuary had the highest levels of total lead concentrations and the lowest activity of ALA-D. Lead isotope analyses demonstrated the different sources of lead contamination and also confirmed the influence of Ría of Huelva on the metal input to the marine environment.     

Flare and filament activation in an unusually distorted field configuration

Using photospheric and H observations and total radio flux data we study a two-ribbon flare in AR NOAA 4263 which was a part of a flare event complex on July 31, 1983. We find some facts which illuminate the special way of flare triggering in the analysed event. Around a double spot the photospheric vector magnetic field is discussed with respect to the chromospheric activities. In one of the spots the feet of long stretched loops are pushed down under steepening loops rooted in the same spot. This causes energy build-up by twist and shear in the stretched loops. One foot of the two-ribbon flare (triggered in the stretched and underpushed loop system) roots in a part of the spot umbra and penumbra where the field runs in extremely flat like a pressed spiral spring. A strange radio event, starting before the flares, can be interpreted as a precursor activity of the flare event complex. The radio data support the view that the analyzed flare process and the given magnetic field structure, respectively, are not very effective in energetic particle generation and escape.     

TV Meteor Observations from Modra

We present our experience and initial results of single-station observation using the new fish-eye TV system, as well as double station TV observation of the Geminids 2006 shower. The fixed fish-eye TV system was developed for monitoring meteor activity throughout the year. We discuss the astrometric precision of our observations using the UFOAnalyser software.     

Possible spectral diagnostics for turbulent electric fields in solar flares

A high level of electrostatic turbulence can be generated by plasma instabilities in solar flares. The turbulence, in general, has both quasistatic (ionic) and dynamic (electronic) components. Hydrogenic line profiles develop distinct features under the simultaneous effect of the quasistatic and dynamic turbulent electric fields: these features are discussed and the possible inferences that can be drawn as to the nature of the turbulent electric fields through their observation are pointed out. Solar flare spectral diagnostics along these lines can provide an observational test of the existence of turbulent electric fields in the flare region. When a detailed determination of the characteristics of these fields is feasible, it would, in turn, help in identifying the underlying plasma instability mechanisms that give rise to these fields during the flare build-up and associated processes.