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排序方式: 共有102条查询结果,搜索用时 15 毫秒
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Comparative oxygen radical formation and toxicity of BDE 47 in rainbow trout cell lines 总被引:2,自引:0,他引:2
The polybrominated diphenyl ethers (PBDEs) constitute a class of flame retardants whose residues have markedly increased in fish and human tissues during the last decade. In particular, the levels of certain PBDE congeners in salmon have raised concern regarding potential risks associated with dietary PBDE exposures. However, little is known regarding PBDE-mediated cell injury in relevant in vitro cell models. We conducted a comparative study of oxyradical production and cell injury in rainbow trout gill (RTgill-W1) and trout liver cells (RTL-W1) exposed to 2,2',4,4'-tetrabromodiphenyl ether (BDE 47), a predominant BDE residue found in fish tissues such as salmonids. Exposure to low micromolar concentrations of BDE 47 elicited a significant loss in RTgill-W1 and RTL-W1 cell viability as measured by alamarBlue assay. The dose-response of BDE toxicity differed among the two cell lines, with the RTL-W1 liver cells showing greater resistance to toxicity at lower BDE 47 doses, but a more dramatic loss of viability relative to gill cells when challenged with higher (50 microM) doses. The sensitivity of the trout liver cells at higher BDE 47 exposures was reflected by a higher basal production of oxygen radical production by 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescence that was markedly enhanced in the presence of BDE 47, suggesting an overwhelming of trout liver cell antioxidant defense pathways. Collectively, our data indicate that RTgill-W1 and RTL-W1 liver cells are sensitive to BDE 47-mediated cell injury through a mechanism that may involve oxidative stress. Our data also provide an in vitro basis for potential tissue differences in BDE 47-mediated cell injury. 相似文献
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ROZINA Tinkara ELER?EK Tina Maja ZUPAN?I? JUSTIN MEGLI? Andrej LE?TAN Domen SEDMAK Bojan 《中国海洋湖沼学报》2018,(4)
The increasing occurrence of cyanobacterial blooms in water bodies is a serious threat to the environment. Efficient in-lake treatment methods for the control of cyanobacteria proliferation are needed, their in-vivo detection to obtain a real-time response to their presence, as well as the information about their physiological state after the applied treatment. In-vivo fluorescence measurements of photosynthetic pigments have proved to be effective for quantitative and qualitative detection of phytoplankton in a water environment. In the experiment, chlorophyll and phycocyanin fluorescence sensors were used concurrently to detect stress caused by electrochemical oxidation applying an electrolytic cell equipped with borondoped diamond electrodes on a laboratory culture of cyanobacteria Microcystis aeruginosa PCC 7806. The inflicted injuries were reflected in a clear transient increase in the phycocyanin fluorescence signal(for 104 %? 43%) 24 h after the treatment, which was not the case for the chlorophyll fluorescence signal. In the next 72 h of observation, the fluorescence signals decreased(on 40% of the starting signal) indicating a reduction of cell number, which was confirmed by cell count(24% reduction of the starting concentration) and analysis of extracted chlorophyll and phycocyanin pigment. These results demonstrate the viability of the combined application of two sensors as a useful tool for in-vivo detection of induced stress, providing real-time information needed for the evaluation of the efficiency of the in-lake treatment and decision upon the necessity of its repetition. The electrochemical treatment also resulted in a lower free microcystins concentration compared to control. 相似文献
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本文介绍一种最近由作者在美国研制的,由计算机产生等距离区域分界线的程序及其数据结构,同时给出初步的试验结果。 相似文献
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地形图等高线成组综合的试验 总被引:15,自引:0,他引:15
费立凡 《武汉大学学报(信息科学版)》1993,(Z1)
本文在讨论制图自动综合一般问题的基础上,着重分析了两种等高线自动综合的技术路线存在的优缺点。笔者提出,采用模拟人类专家智能的方法,从二维的等高线输入数据出发,提取地貌结构信息,从而对等高线进行成组的综合,直接形成经综合的二维等高线输出数据,有可能获得较为简捷,效果良好的综合结果。在对这条技术路线进行实验的过程中所涉及的人工智能问题,主要包括:对无序数字化等高线数据的整理、等高线上重要特征点的识别、正负向地貌特征的确定、谷地点的选出、谷底线的自动跟踪、谷地重要性的计算、谷间距的自动量算、对称谷地的寻找、综合后图形的生成等。 相似文献
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本文讨论了机助制图中图形数据的开窗及其程序设计的有关实际问题。同时,针对矢量数据开窗处理的特点,讨论了为提高计算效率可采用的一些算法。 相似文献