Histopathological biomarkers in estuarine fish species for the assessment of biological effects of contaminants

The increasing emphasis on the assessment and monitoring of estuarine ecosystems has highlighted the need to deploy appropriate biological indices for these locations. Fish diseases and histopathology, with a broad range of causes, are increasingly being used as indicators of environmental stress since they provide a definite biological end-point of historical exposure. This study reports on the histopathological alterations observed in selected organs and tissues of three species of estuarine fish (Platichthys flesus, Pomatoschistus minutus and Zoarces viviparus), captured from four British estuaries (the Tyne, Tees, Mersey and Alde), differently impacted by contaminants, including PAHs. A biannual sampling regime was used to identify the important seasonal variations that occur in terms of the observed biological effects. Inflammatory lesions and hepatocellular fibrillar inclusions attained their highest prevalence in P. flesus captured from the Tyne, Tees and Mersey. The presence of pre-neoplastic and neoplastic toxicopathic lesions was highest in P. flesus captured from these sites, when compared to fish from the Aide reference site. In particular, the prevalence of hepatic foci of cellular alteration (up to 43.3%) and hepatocellular adenoma (up to 10%) were highest in P. flesus captured from the Mersey estuary. Intersex (ovotestis) was only recorded in male P.flesus captured from the Mersey estuary (up to 8.3%) and from male Z. viviparous captured from the Tyne estuary (25%). Pathologies associated with the gill and the kidney were also most prevalent in fish captured from the Tyne, Tees and Mersey estuaries. This study has successfully applied histopathology to an estuarine monitoring program, both for the recording of toxicopathic lesions in the liver and other organs, and for the detection of the endpoint of endocrine disruption, intersex. As such, it provides a powerful integrative tool for the assessment of biological effects of contaminants in these environments.     

Ageing research in Australia: reflecting on Graeme Hugo’s four decades of contribution

ABSTRACT

Globally, population ageing is one of the most pressing social and policy issues faced today. Over the next two decades, Australian society will face dramatic increases in the proportion of the population aged 65 years and over, as the baby boomers move into older age and fertility levels remain low. Yet population ageing is not a surprising or new trend—demographic changes in the age profile of a population tend to occur incrementally rather than suddenly. As a demographer and geographer, Graeme Hugo drew attention to this trend in Australia’s population more than three decades ago. Throughout Graeme Hugo’s vast breadth of work over the past 40 years, there has been a consistent thread of demographic analysis and academic thought associated with the ageing of Australia’s population. This paper focuses on Hugo’s contributions to academic thought and policy on Australia’s ageing population and the challenges associated with this for both service delivery and health policy as Australian society moves into an unprecedented era of population ageing.     

The impact of oestrogenic and androgenic contamination on marine organisms in the United Kingdom--summary of the EDMAR programme. Endocrine Disruption in the Marine Environment

This paper summarises results of the EDMAR programme which is investigating oestrogenic and androgenic endocrine disruption in UK coastal waters. Most of the data concern fish. Four species (flounder, viviparous blenny and two sand gobies) are experiencing feminisation in industrialised estuaries. In males this includes vitellogenin (VTG) synthesis, ovotestis induction and/or feminised sexual characteristics. Although reproductive success may be impaired in some cases, implications for fish populations are still unclear. Suspected causative contaminants include natural oestrogenic substances and synthetic oestrogen mimics. The majority of the oestrogenic activity is adsorbed to sediments, and routes of exposure may include benthic food chain transfer. Some natural androgenic substances are also being discharged to estuaries, but their activity appears low.     

Charophytes du Crétacé moyen et données nouvelles sur l'Evolution des Clavatoracées

La découverte de nouveaux gisements dans le Sud de la France révèle la persistance, dans le Cénomanien, d'Embergerella cruciata, Atopochara restricta et A. brevicellis (Clavatoraceae). Une parenté entre les genres Embergerella (Barrémien-Cénomanien) et Septorella (Maastrichtien) est envisagée sur la base d'une nouvelle interprétation de la structure de l'utricule. Pour la première fois, des représentants de la famille des Characeae sont décrits dans le Cénomanien: Peckisphaera revesti n. sp. et le Turonien: Mesochara fusiformis n. sp. Indications biostratigraphiques: dans l'état actuel des connaissances sur les flores de Charophytes du Crétacé moyen, seul le Cénomanien est bien caractérisé, par A. multivolvis à laquelle s'ajoute maintenant une nouvelle association de quatre espèces; l'Albien et le Turonien, pour lesquels des précisions sont apportées, restent toutefois encore mal individualisés.The discovery of new localities in the South of France shows that Embergerella cruciata, Atopochara restricta and A. brevicellis (Clavatoraceae) continued into the Cenomanian. A relationship between the genera Embergerella (Barremian-Cenomanian) and Septorella (Maastrichtian) is suggested on the basis of a new interpretation of the structure of the utricle. Representatives of the family Characeae are described for the first time, Peckisphaera revesti n. sp. in the Cenomanian and Mesochara fusiformis n. sp. in the Turonian. Biostratigraphic information: in the present state of knowledge of Middle Cretaceous Charophyte floras, only the Cenomanian is well characterized, by A. multivolvis to which is now added a new association of four species. Although new information is presented on the Albian and Turonian, they are still inadequately understood.     

The viviparous blenny (Zoarces viviparus) as a bioindicator of contaminant exposure: application of biomarkers of apoptosis and DNA damage

The viviparous blenny (Zoarces viviparus), also known as eelpout, is considered a suitable biomonitor for use in European estuarine and coastal waters. We have previously shown that Z. viviparus from the industrialised Tyne estuary (UK) display certain histopathologies (e.g., ovotestis and hepatic nuclear and cellular pleomorphism), which are associated with contaminant exposure [Mar. Environ. Res. 55 (2003) 137]. Furthermore, the prevalence of these pathologies was higher than in fish collected from a less contaminated reference site (the Alde estuary, UK). Here, tissue samples were collected from Z. viviparus from the Tyne and Alde estuaries (in the spring and autumn) and analysed for DNA adducts using the (32)P-postlabelling assay and for histopathology. Z. viviparus caught from the Tyne during the spring (56.4+/-18.5 adducts per 10(8) nucleotides) and autumn (63.1+/-7.5 adducts per 10(8) nucleotides) exhibited patterns of DNA adducts which indicated exposure to a complex mixture of genotoxins. In contrast, levels of DNA adducts in Z. viviparus from Alde estuary were significantly lower during both sampling periods. Histopathological changes to the gonads included intersex (ovotestis) and germ cell apoptosis (detected via the TUNEL assay) in male fish captured from the Tyne estuary. Further studies on the use of germ cell apoptotic rate at different estuarine sites are required if it is to be used as a reproductive biomarker of contaminant exposure in male fish.     

Sedimentology, palaeontology, biostratigraphy and correlation of the Late Cretaceous Vilquechico Group of southern Peru

Detailed analysis of the Late Cretaceous Vilquechico Group (formerly Vilquechico Formation) of the Southern Andes allows the recognition of three major sedimentary sequences, defining Lower, Middle and Upper Vilquechico lithologic formations (LVF, MVF and UVF respectively). Some of them (MVF and UVF) include in turn minor sedimentary sequences. In addition to dinosaur trackways, they contain a marine fauna (selachians, actinopterygians, molluscs) in their transgressive basal parts, and lacustrine fossils (charophytes, ostracods, gastropods) in their regressive continental upper parts. Two charophyte biozones characterize the MVF and the UVF respectively. The lithologic and sedimentary features of the major sequences, as well as their palaeontological contents allow large-scale correlations with other Andean series. Such correlations permit us to tentatively ascribe the unfossiliferous LVF to the Coniacian-early Santonian (?) time-span, and the MVF to the Santonian late Campanian interval. The UVF is of latest Campanian-late Maastrichtian age. As a consequence, the assumed correlations between the Vilquechico Group and some of the vertebrate-bearing Andean localities are revised.     

Water column monitoring near oil installations in the North Sea 2001-2004

Fisheries have been vital to coastal communities around the North Sea for centuries, but this semi-enclosed sea also receives large amounts of waste. It is therefore important to monitor and control inputs of contaminants into the North Sea. Inputs of effluents from offshore oil and gas production platforms (produced water) in the Norwegian sector have been monitored through an integrated chemical and biological effects programme since 2001. The programme has used caged Atlantic cod and blue mussels. PAH tissue residues in blue mussels and PAH bile metabolites in cod have confirmed exposure to effluents, but there was variation between years. Results for a range of biological effects methods reflected exposure gradients and indicated that exposure levels were low and caused minor environmental impact at the deployment locations. There is a need to develop methods that are sufficiently sensitive to components in produced water at levels found in marine ecosystems.     

Expansion rates and recruitment frequency of exotic smooth cordgrass, Spartina alterniflora (Loisel), colonizing unvegetated littoral flats in Willapa Bay, Washington

We estimated lateral growth rates of nonindigenous Spartina alterniflora patches in Willapa Bay, Washington, over three intervals between 1970 and 1990, to reconstruct recruitment frequency since S. alterniflora was initially introduced c. 1890. Black and white aerial photographs (1∶24,000 scale) of four representative sites where S. alterniflora has invaded were analyzed. Individual patches were digitized on a computer, and diameter and area were computed for each patch for all years and sites sampled using CAD software. Lateral growth rates of individual. S. alterniflora patches increased linearly at 79.3 (± 1.674 SE) cm yr⁻¹. Back calculations of origin dates for individual patches based on mean lateral growth rates indicate that recruitment of new patches has been episodic and increasing in frequency since initial introduction. Environmental factors such as sea surface temperature, sea level, and precipitation may account for some of this recruitment variability. These data may be useful in existing and future models of S. alterniflora expansion in Pacific Northwest estuaries.     

Exposure of sticklebacks (Gasterosteus aculeatus) to cadmium sulfide nanoparticles: biological effects and the importance of experimental design.

Effects of nanoparticles on aquatic organisms have been little studied to date and toxicological data are urgently needed for development of regulatory frameworks for these substances. Here, we report the findings of a study exposing sticklebacks to cadmium sulfide (CdS) as bulk material and quantum dots. Fish were exposed for 21 d in a flow through test system to 5, 50 or 500 microg l(-1) CdS nanoparticles (nCdS) coated in thiol terminated methyl polyethylene glycol (MPEG), bulk CdS or MPEG at 500 microg l(-1) (nominal concentrations). With the exception of the highest nCdS exposure, measured concentrations were approximately one order of magnitude below nominal. A single fish from each group (excluding MPEG) was examined using energy dispersive X-ray analysis (EDX) to localise cadmium, however, cadmium could not be detected in whole body sections. Elevated levels of oxidized glutathione were measured in the gills of fish exposed to 50 and 500 microg l(-1) nCdS. Induction of vitellogenin synthesis was not detected in any of the treatment groups. The number of males engaged in nest-building behaviour following exposure to 500 microg l(-1) nCdS was reduced and livers of 4/6 fish in the same treatment displayed hepatocellular nuclear pleomorphism. The results are discussed emphasising the fundamental importance of experimental design and the need to understand the behaviour of nanoparticles in the aqueous phase.     

Transforming management of tropical coastal seas to cope with challenges of the 21st century

Over 1.3 billion people live on tropical coasts, primarily in developing countries. Many depend on adjacent coastal seas for food, and livelihoods. We show how trends in demography and in several local and global anthropogenic stressors are progressively degrading capacity of coastal waters to sustain these people. Far more effective approaches to environmental management are needed if the loss in provision of ecosystem goods and services is to be stemmed. We propose expanded use of marine spatial planning as a framework for more effective, pragmatic management based on ocean zones to accommodate conflicting uses. This would force the holistic, regional-scale reconciliation of food security, livelihoods, and conservation that is needed. Transforming how countries manage coastal resources will require major change in policy and politics, implemented with sufficient flexibility to accommodate societal variations. Achieving this change is a major challenge – one that affects the lives of one fifth of humanity.