细角螺微卫星DNA 富集文库构建及特征分析

采用生物素磁珠富集法,用生物素标记的(GT)15和(CT)15两种探针与细角螺基因组 MseI 酶切的300~1200bp 片段杂交,杂交复合物与链霉亲和素磁珠结合,捕获含有重复序列的微卫星片段。最后将磁珠捕获到的重复序列与PMD19-T载体连接后克隆到DH5α中构建微卫星基因组文库。通过PCR检测出354个阳性克隆,从中随机选取248个片段大于500bp的阳性克隆进行测序,结果显示,在220个成功测序的阳性克隆中共获得278个微卫星序列,其中完美型171个,占61.5%;非完美型81个,占29.1%;复合型26个,占9.4%。除探针使用的GT和CT的重复序列外,还筛选到三碱基GTT、TGG、GAA、CAA;四碱基TCTA、ACAG、TAGA、GTGA、GTCT、GATA及五碱基TTTTG的重复序列。在278条序列中共有82条可以设计引物。     

GF-1WFV与Landsat8OLI的NDVI交互比较与定量关系研究

为弥补、修正构建Landsat 8归一化植被指数(normalized differential vegetation index,NDVI)长时间序列中因云层覆盖、影像质量与重访周期导致的数据缺失问题,交互比较高分一号(GF-1)与Landsat 8两类传感器的植被探测能力,并对其植被指数的定量关系进行研究,探讨以GF-1局部镶嵌或全局替换Landsat 8缺失数据构建时序NDVI的可行性。结果显示,Landsat 8与GF-1具有NDVI差异性,具体表现为Landsat 8探测地物信号更强,而GF-1获取地物信息量更多;相同空间分辨率(30 m)下两传感器NDVI数据呈现高度线性相关,经方程转换后,GF-1的NDVI拟合数据与Landsat 8的NDVI关系增强;同时,两传感器的NDVI数据存在区域差异性,且拟合数据可以有效减少差异程度。实验表明,此方法可实现Landsat 8高分辨率时序NDVI的构建,具有一定应用价值。     

Analysis of the genetic diversity and differentiation of <Emphasis Type="Italic">Fenneropenaeus penicillatus</Emphasis> populations using AFLP technology

Fenneropenaeus penicillatus (redtail shrimp) is an important marine commercial animal in China. Recently, its resources have been depleted rapidly as a result of, for example, over-exploitation and environmental degradation of spawning grounds. Therefore, we analyzed the genetic diversity and differentiation of nine wild populations of F. penicillatus of China (Ningde, Lianjiang, Putian, Xiamen, Quanzhou, Zhangpu, Dongshan, Nanao, and Shenzhen populations) by amplified fragment length polymorphism (AFLP) technology, to provide genetic information necessary for resource protection, rejuvenation, artificial breeding, and sustainable use of the resource. Eight AFLP primer pairs were used for amplification, and 508 bands were detected among the populations. The results show that the percentage of polymorphic loci (P) ranged from 41.34% to 63.58%; the Nei’s gene diversity (H) of the populations was 0.119 4-0.230 5; and Shannon’s Information Index (I) was 0.184 1-0.342 5. These genetic data indicate that the genetic diversity of F. penicillatus was high. The genetic differentiation coefficient (GST=0.216 2) and gene flow (Nm=1.812 4) show that there was a high level of genetic differentiation and a moderate level of gene flow among populations. More studies on the genetic differentiation mechanism of F. penicillatus along the south-eastern coast of China need to be conducted to find more effective scientific protection strategies for the conservation of F. penicillatus genetic resources.