Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits in Fenneropenaeus chinensis

Amplified fragment length polymorphism (AFLP) technique was used to analyze the fingerprinting of four successive generations ofFenneropenaeus chinensis to reveal their disease-resistance traits. Some loci showed quite different genetic frequencies due to artificial selection, which implied that these fragments were putative markers related to the disease-resistance trait. We developed a simple and effective method to further characterize these AFLP fragments. Specific AFLP bands were cut directly from polyacrylamide gels, re-amplified, cloned and sequenced. Eight putative genetic markers were sequenced and their sizes ranged from 63 to 209 bp. The sequences were submitted to dbGSS (database of Genome Sequence Survey); and the BLAST analysis showed low similarity to the function genes, indicating these markers were tightly linked to a disease-resistance trait but were not functional genes. This research was supported by special funds from the National Key Basic Research Program (G1999012007) and the National High-Tech Research and Development Program of China (863 Program. 2001AA620105).     

值得重视的海域海相油气勘探

当前我国海域油气的勘探领域和勘探层位大部分局限于新生界,而对古生代海相地台沉积、特提斯海相沉积、南海生物礁、南海和冲绳等深水盆地领域的勘探涉足不深。通过海域海相油气的地质调查与研究以及新区评价,认为在我国海域拓宽找油的战略选区,力争海域海相油气勘探获得突破、发现储量规模大的整装油田是有可能的。