石斑鱼虹彩病毒SGIV在宿主细胞内依赖微管运动的行为特征

病毒是非常微小的简单生物, 不能独立生存, 必须借助宿主细胞完成自身的繁衍。病毒侵染进入细胞后, 通常借助于微管通过黏稠的细胞质运动到特定的复制位点。然而, 有关病毒依赖微管运动行为的精细动态研究还比较少。石斑鱼虹彩病毒(Singapore grouper iridovirus, SGIV)为虹彩病毒科蛙病毒属的一个新种, 是海水养殖鱼类的重要病毒性病原, 对海水养殖业造成重大经济损失。利用单粒子示踪技术实时追踪了SGIV病毒粒子沿微管运动的行为, 观察到SGIV在细胞边缘至微管中心之间的双向运动, 最高瞬时速度约0.2μm·s^-1, 均表现为主动运输。病毒粒子运动至微管交叉位置会减速迂回, 而后或受限于此, 平均运动速率约0.008μm·s^-1, 或通过交叉处继续快速运动, 最高瞬时速度为0.2μm·s^-1。同时, SGIV感染会影响微管的形态结构, 随着SGIV感染, 微管逐渐围绕细胞核和病毒加工厂形成环状结构。研究结果初步揭示了SGIV病毒和细胞微管之间相互作用的复杂过程, 丰富了我们对虹彩病毒胞内生命活动的认识, 有助于深入地理解海水鱼类虹彩病毒感染致病机理。     

基于四维灾体模型的浙江省气象灾害损失年景评价

应用浙江省气象灾情统计年鉴资料,运用四维灾体评估模型,对浙江省的气象灾害损失进行科学评估。浙江省2003—2017年灾害损失年景评价分析结果显示,2004年和2005年为气象灾害损失明显偏重年景,进入2010年以来,2013年为年景最差年份;台风灾害损失在每年浙江省气象灾害损失年景评价中具有非常重要的作用,重大台风事件易造成气象灾害偏重或明显偏重的损失年景。评估结果表明,四维灾体模型下的气象灾害评估较为科学客观。     

产过氧化氢酶菌株CE-1 的筛选与鉴定

经过含H2O2的平板初筛和摇瓶发酵复筛,从保存的水样中筛选到一株过氧化氢酶高产菌株CE-1,其所产过氧化氢酶活性和比活分别为1 356.2 U/mL和3 401 U/mg。菌株CE-1为革兰氏阴性杆状细菌,根据其形态特征、16S rRNA基因序列系统进化树分析和生理生化特性,以及属内不同种间性状差异等因素的综合分析,将菌株CE-1鉴定为气单胞属(Aeromonas)细菌。     

Spatial Variation of Sea Level Trend Along the Bangladesh Coast

The Bangladesh coast is threatened by rising sea level due to various factors. The results based on the analysis of past 22 years of tidal data of the Bangladesh coast reveal that the annual mean tidal level in the eastern Bangladesh coast is rising at an alarmingly high rate of 7.8 mm/year, which is almost twice the observed rate in the western region. This type of sea level trend seems to be the result of changing local conditions like increased precipitation and land subsidence during the recent decades. It seems that the higher rate of land subsidence in the eastern Bangladesh coast is the main causative factor for the steeper sea level trends there. The differential sea level trends show that the subsidence component in the sea level rise may be as high as 4 mm/year in the eastern Bangladesh coast. However, this needs to be verified with actual geological observations.     

养殖大黄鱼在自然海区降温不同阶段抗氧化水平及血清酶活性的变化

为了解越冬期间水温下降对大黄鱼抗氧化水平和血清酶活性的影响, 作者研究了养殖大黄鱼(Pseudosciaena crocea)冬季海区自然降温不同阶段(20、16、12、10、8℃)肝脏和肌肉中抗氧化水平及血清酶活性的变化。结果显示: 自然降温过程中, 肝脏中3 种抗氧化酶变化趋势不同, 其中SOD 活性呈升高趋势, 8℃时SOD活性最强; POD 活性呈先升高后降低的变化趋势, 16℃时活性最高; CAT 活性呈下降趋势; 而肌肉中这3 种抗氧化酶活性均呈下降趋势。肝脏中GSH 和MDA 含量在16℃时最高; 肌肉中的3 种抗氧化酶活性、GSH 和MDA 含量以及总抗氧化能力(T-AOC)均明显低于肝脏中, 反映了肝脏对清除自由基具有重要作用, 在抗氧化调节方面起到主要作用。血清酶ALT、AST、ALP、LDH 及CK-MB 随水温自然下降酶活性均呈现降低趋势, 即20℃时酶活性最强; CK 和LIP 随着水温降低, 呈先上升再下降的趋势; ADA 和GGT 随水温降低活性呈上升趋势。血清酶的不同变化, 说明水温对大黄鱼血清酶活性有一定的影响。     

改进的移动粒子半隐式法模拟楔形体入水砰击

移动粒子半隐式法(Moving-Particle Semi-Implicit Method,MPS)是一种新的基于拉格朗日(Lagrange)理念的无网格方法,适用于模拟自由液面的大变形和水流的喷射现象。用基于大涡模拟的改进MPS法首先模拟了矩形体的入水砰击,砰击压力的计算结果证明了这种方法的正确性,然后模拟了楔形体的匀速入水砰击,并与实验结果进行了对比,验证了大涡模拟改进MPS法在砰击问题中的适用性。     

沙箱对桩腿耦合装置的影响研究

桩腿耦合装置(简称LMU)在平台浮托安装过程中上部组块插尖与导管架桩管对接期间起缓冲作用,是海上浮装的最重要装置之一。运用非线性有限元软件ABAQUS,对相同尺寸、受相同垂向载荷作用的有沙箱和无沙箱的LMU分别进行了显式准静态和动态数值模拟,并将计算结果进行对比,以考察沙箱对LMU力学特性的影响。结果表明,与无沙箱的LMU相比,有沙箱的LMU能够承受较大的外载荷作用,可提升外套筒钢材的使用效率,较迅速地降低载荷转移作业过程中整个平台的运动速度,提高平台浮托安装过程中能量吸收速率,并且能减小LMU在动态过程中的最大应力。     

Water quality in a polluted lowland stream with chronically depressed dissolved oxygen: Causes and effects

Abstract

The Whangamaire Stream (North Island, New Zealand) has high concentrations of nitrate nitrogen (NO^? ₃‐N), biochemical oxygen demand (BOD₅), and Kjeldahl nitrogen (TKN) as a result of catchment land use practices. The lower reaches of the stream drain intensively farmed land and have dissolved oxygen (DO) levels of 10–50% saturation. The dominant riparian vegetation, Apium nodiflorum, provides a large organic loading by intercepting nutrients in run‐off and then decaying in the stream channel. Water quality and reaeration aspects of the stream were studied in order to explain the observed low DO levels. Measurements of the reaeration coefficient at 20°C, K₂ ²⁰, using methyl chloride (CH₃Cl) as a gas tracer, yielded values of 1.1–3.0 d^?1 for the upper part of the study reach and 15.5–16.2 d^?1 for the lower reach (overall average 12.5 ± 2.5 d^?1). These were in agreement with values inferred from single‐station diurnal curve analysis, which also showed that respiration was dominant in the lower reach where photo‐synthetic activity was inhibited by shade. The relatively large reaeration coefficients ensure that parts of the stream do not become anoxic at night time. Better riparian management and reduced nutrient inputs are likely to improve stream water quality.     

On the Conversion of Antarctic Ice-Mass Change to Sea Level Equivalent

This technical note aims to provide a quick reference and some computational examples for the conversion between Antarctic ice-mass changes and global sea level equivalent (SLE) changes using a few assumptions that computationally simplify this complex problem and that acknowledge gaps in our knowledge of the Antarctic environment. A number of factors involved in the conversion process are discussed, and the sensitivity of the conversion result to certain aspects is analyzed. It was found that the global ocean area calculated from a recently improved global shoreline dataset has little impact on the uncertainty of the SLE estimation. SLE estimation using satellite gravity observations, such as those by GRACE, are sensitive to glacial isostatic adjustment (GIA) models. One more important result from the computation is that the effective density of the volume that is gained or lost during mass change may greatly affect the outcome of the conversion if it differs greatly from the actual density of the firn/ice layers. Finally, a table of computational examples is provided for reference under some assumptions for simplifying the computation.     

Behavior of soil plugs in open‐ended model piles driven into sands

Calibration chamber tests were conducted on open‐ended model piles driven into dried siliceous sands with different soil conditions in order to clarify the effect of soil conditions on load transfer mechanism in the soil plug. The model pile used in the test series was devised so that the bearing capacity of an open‐ended pile could be measured as three components: outside shaft resistance, plug resistance, and tip resistance. Under the assumption that the unit shaft resistance due to pile‐soil plug interaction varies linearly near the pile tip, the plug resistance was estimated. The plug capacity, which was defined as the plug resistance at ultimate condition, is mainly dependent on the ambient lateral pressure and relative density. The length of wedged plug that transfers the load decreases with the decrease of relative density, but it is independent of the ambient pressure and penetration depth. Under several assumptions, the value of earth pressure coefficient in the soil plug can be calculated. It gradually reduces with increase in the longitudinal distance from the pile tip. At the bottom of the soil plug, it tends to decrease with increase in the penetration depth and relative density, and to increase with the increase of ambient pressure. This may be attributed to (1) the decrease of friction angle as a result of increase in the effective vertical stress, (2) the difference in the dilation degree of the soil plug during driving with ambient pressures, and (3) the difference in compaction degree of soil plug during driving with relative densities. Based on the test results, an empirical equation was suggested to compute the earth pressure coefficient to be used in the calculation of plug capacity using one‐dimensional analysis, and it produces proper plug capacities for all soil conditions.