A cDNA microarray technique applied for analysis of global gene expression profiles in tributyltin-exposed ascidians

To analyze global gene expressions, we constructed a cDNA microarray from a basal chordate, the ascidian Ciona intestinalis. Ciona is a cosmopolitan species and a genomic analysis of Ciona revealed that ascidians had approximately 15,500 protein-coding genes. Our "Ciona intestinalis cDNA chip version 1 (Ci cDNA chip ver. 1)" has arrayed 13,400 unique Ciona cDNAs. To establish a detection system for gene expression profiles in wild ascidians using a cDNA microarray, we analyzed gene expressions in the whole body of Ciona adults after exposure to 100 nM tributyltin (TBT) for 24 h. In our preliminary array data using Ci cDNA chip ver. 1, we found more than 200 genes that showed strong differential expressions. These genes encoded proteins that were concerned with stress response, detoxification, oxidoreduction reaction, biosynthesis, and catabolism. This, the first large cDNA microarray of this animal, should facilitate analyses of global gene expressions following exposure to TBT.     

土水特征曲线的通用数学模型研究

土水特征曲线对于研究非饱和土的物理力学特性至关重要。根据土水特征曲线可以确定非饱和土的强度、体应变和渗透系数 ,甚至可以确定地下水面以上水份分布。由于土体物理力学特性的差异 ,导致描述其土水特征曲线的数学模型也各不相同。因此 ,建立土水特征曲线的通用数学表达式 ,显得尤为必要。本文对土水特征曲线数学模型进行了研究 ,依据这些模型的数学表达式形式 ,将其划分为 4种类型。分别由这 4种类型的数学模型推导出具有统一表达式形式的土水特征曲线通用数学模型 ,并运用陕北高原黄土土水特征曲线试验数据对通用数学模型进行了研究     

憧憬未来与"爱""恨"交织的时代之歌——柳亚子辛亥革命前后诗词创作浅论

柳亚子是辛亥革命前后诗坛上影响最大的革命诗人之一.他在辛亥革命前后创作的大量诗词,追求理想,憧憬未来;关注现实,"爱"恨"交织;并以慷慨激昂,苍凉沉郁调子组成一曲曲激荡着宏音巨响的时代之歌,在中国近代的茫茫夜空中激荡震响,这些裹夹着雷霆风云与宏音巨响的诗歌创作在我国民主革命史和近代文学史上产生了重要影响,并放射出璀璨的光辉.     

Optimization of the purification methods for recovery of recombinant growth hormone from Paralichthys olivaceus

This study aimed to optimize the purification of recombinant growth hormone from Paralichthys olivaceus. Recombinant flounder growth hormone (r-fGH) was expressed by Escherichia coli in form of inclusion body or as soluble protein under different inducing conditions. The inclusion body was renatured using two recovery methods, i.e., dilution and dialysis. Thereafter, the refolded protein was purified by Glutathione Sepharase 4B affinity chromatography and r-fGH was obtained by cleavage of thrombin. For soluble products, r-fGH was directly purified from the lysates by Glutathione Sepharase 4B affinity chromatography. ELISA-receptor assay demonstrated that despite its low receptor binding activity, the r-fGH purified from refolded inclusion body had a higher yield (2.605 mgL-1) than that from soluble protein (1.964 mgL-1). Of the tested recovery methods, addition of renaturing buffer (pH 8.5) into denatured inclusion body yielded the best recovery rate (17.9%). This work provided an optimized purification method for high recovery of r-fGH, thus contributing to the application of r-fGH to aquaculture.     

整合多源异构的城市规划数据进行认知交流的研究

针对整个城市规划过程的参与者对规划数据的不同种类、不同层次认知需求,本文利用本体思想对包括GIS数据、规划文档、规划图件等多源异构的规划数据进行关系重构,设计本体模型要素的数据语义关系,定义数据间的关联关系,实现基于本体方法的规划领域语义表达算法,方便了各类规划参与者对规划方案认识的交流:同时根据用户特征分类,进行本体关系的逻辑推理,过滤冗余信息,进行多源异构规划数据的可视化。     

Reproductive Biology of Yeilowfin Tuna T. albacares in the West-Central Indian Ocean

Survey of yellowfin tuna in the west-central Indian Ocean was conducted on board of Chinese Iongliners during 2003, 2004 and 2005, which is a part of Chinese Tuna Fishery Scientific Observer Program (CTFSOP). The reproductive biology has been investigated. A total of 1023 samples are collected including 417 ovaries and 606 testes. Spawning activities of yellowfin tuna have been studied for both male and female from January to June. The data showed that the average monthly sex ratio is 0.59, and the minimum length at sexual maturity is 101 cm for female and 110 cm for male respectively. Length at 50% sexual maturity is esti- mated at 113.77 cm for female and 120.20 cm for male, whereas maturation rate is 0.066 cm-1 for female and 0.091 cm-1 for male. Sex ratio by length class indicates that the proportion of male is higher than female's along with size increasing; for instance, in the group of the body length longer than 145 cm, some females have their body length from 145 to 160 cm and males have their body length at 160 cm and even longer. Statistically, yellowfin tuna has a significant seasonal reproduction.     

航海英语语义探源

在世界航海过程中 ,人们创造了众多语言词汇 ,丰富了人类语言。英语中也有许多与航海有关的词语 ,它们语义的产生有其特定的背景 ,在使用过程中被赋予新的含义 ,至今广泛使用。     

凯氏拟小球藻己糖激酶基因的克隆及其在不同培养条件下的表达分析

为研究凯氏拟小球藻(Parachlorellakessleri)糖代谢分子机制,本研究采用RT-PCR和RACE技术从凯氏拟小球藻中克隆了己糖激酶基因CkeHK(GenBankID:AHF54566),并对其自养、异养、混养条件下的转录表达进行分析。结果表明,该序列的cDNA全长为1844bp,开放阅读框1389bp,编码462个氨基酸。该蛋白的相对分子质量为49.73,等电点为6.98。实时荧光定量PCR结果显示,以自养培养条件为对照,异养培养和混养培养条件下,CkeHK均能够发生明显上调,且混养条件下上调量比异养条件下上调量更多,说明CkeHK可能在凯氏拟小球藻利用外源糖的过程发挥重要作用,并且光信号对于凯氏拟小球藻利用外源糖可能存在调控作用。这些研究结果为进一步阐明CkeHK的功能及其作用机制奠定了分子基础。