柳珊瑚Leptogorgia rigida中甾体类成分研究

首次对南海柳珊瑚Leptogorgia rigida中甾体类次生代谢产物进行研究。采用硅胶柱柱层析、十八烷基硅烷键合硅胶填料、Sephadex LH-20、高效液相色谱(HPLC)及薄层色谱(TLC)等多种方法进行分离纯化, 通过核磁共振波谱(NMR)、质谱(MS)等现代波谱分析及物理常数对照等方法进行结构鉴定。从南海柳珊瑚Leptogorgia rigida次生代谢产物中分离得到8个甾类化合物, 结构依次为: 3β, 6β, 11-trihydroxy-9, 11-seco-5α-chloest-7-en-9-one (1); 3β, 6α, 11- trihydroxy-9, 11-seco-5α-chloest-7-en-9-one (2); 3β, 6α, 11-trihydroxy-5α-chloest-7, 22(E)-dien-9-one (3); pregnan-4-en-3, 20-dione (4); 5α-pregnan-3, 20-dione (5); 3β-hydroxy-pregnan-5-en-20-dion (6); 3β-hydroxy-5α-pregnan-20-one (7); 和3β, 6α, 11-trihydroxy-9, 11-seco-5α-gorgosterol (8)。所有化合物均首次从Leptogorgia属中得到。     

海底玄武岩中次生组分HCl溶解实验研究

次生组分沉淀是海底岩石中普遍存在的现象,次生组分去除是减少次生组分干扰、获取海底岩石高质量数据的关键。选择西太平洋麦哲伦海山区MK海山相对新鲜的玄武岩作为研究对象,经研磨、均一化后将粉末样品分为49份,其中48份分别以0.25,0.50,1.00,1.50,2.00,2.50 mol/L六种浓度的足量HCl溶解0.5,1,2,4,8,16,32和64 h后,选取溶解残渣测定主要氧化物和特征微量元素质量分数,确定HCl溶解法在去除次生组分的同时不会对海底新鲜玄武岩中原生组分造成影响的HCl浓度-时间组合。结果表明,在HCl浓度为1.00～2.50 mol/L,溶解时间为0.5～2 h的12种HCl浓度-时间组合均可保证海底玄武岩中原生组分的安全,并可有效去除次生组分。综合海底蚀变玄武岩次生组分去除实验研究,推荐使用1.00～2.00 mol/L HCl,溶解时间在0.5～1 h的实验方案。     

Ex situ cultivation of Aplysinaaerophoba close to in situ conditions: ecological, biochemical and histological aspects

Sponges provide the largest number of biologically active natural products known from the marine environment and continue to be a very well studied phylum of marine fauna. The Mediterranean sponge Aplysina aerophoba accumulates brominated isoxazoline alkaloids such as Aplysinamisin-1, Aerophobin-2, Isofistularin-3 and the biotransformation product Aeroplysinin-1, which possesses, for example, antibiotic and cytotoxic properties. Until now, it is still being discussed which organisms – the sponge itself or associated microorganisms – are responsible for metabolite production. For cultivating Aplysina individuals under ex situ conditions, we surveyed relevant ecological factors in situ and controlled them in our aquarium system. We maintained A. aerophoba for more than 9 months and analysed changes of metabolite content and composition, microbial association as well as morphology in situ and ex situ under different light exposure. Although sponges showed slight reduction during maintenance, ex situ cultivation similar to in situ conditions provides a promising method to keep sponges and obtain their bioactive metabolites.     

Seismic images of a collision zone offshore NW Sabah/Borneo

Multichannel reflection seismic data from the southern South China Sea, refraction and gravity modelling were used to investigate the compressional sedimentary structures of the collision-prone continental margin off NW Borneo. An elongated imbricate deepwater fan, the toe Thrust Zone bounds the Northwest Borneo Trough to the southeast. The faults separating the individual imbricates cut through post-Early Miocene sediments and curve down to a carbonate platform at the top of the subsiding continental Dangerous Grounds platform that forms the major detachment surface. The age of deformation migrates outward toward the front of the wedge. We propose crustal shortening mechanisms as the main reason for the formation of the imbricate fan. At the location of the in the past defined Lower Tertiary Thrust Sheet tectonostratigraphic province a high velocity body was found but with a much smaller extend than the previously defined structure. The high velocity structure may be interpreted either as carbonates that limit the transfer of seismic energy into the sedimentary layers beneath or as Paleogene Crocker sediments dissected by remnants of a proto-South China Sea oceanic crust that were overthrust onto a southward migrating attenuated continental block of the Dangerous Grounds during plate convergence.     

Difficulties in separating hurricane induced effects from natural benthic succession: Hurricane Isabel,a case study from Eastern Virginia,USA

Hurricane Isabel reached the Eastern seaboard of North America on 18 September 2003 causing estimated damage >3 billion US dollars and the death of ∼50 people. Isabel is considered to be one of the most significant tropical cyclones to affect Virginia, since the Chesapeake Potomac Hurricane of 1933 and Hurricane Hazel in 1954. A study of the temporal changes in the benthic fauna pre- and post-hurricane was conducted on an intertidal sandflat within the dynamic barrier island system near Wachapreague, Eastern Virginia. Replicate sediment cores were collected 3 weeks before Isabel made landfall and further samples were collected on 5 occasions over the following 20 months. An immediate effect of Isabel was a doubling in the number of species, a significant increase in invertebrate species diversity (H′) and a rise in opportunistic species and deposit feeders, but a non-significant increase in the total number of organisms. Changes in infauna occurred such that by the end of the study there were significantly increased numbers of species, faunal abundances and community diversity measures, as compared with pre-hurricane samples, suggesting a potentially positive medium-term effect of this hurricane perturbation. The most notable direct effects of the hurricane were on the relative abundances of feeding guilds with a reduction in interface feeders from 87% pre-hurricane to 64% post-hurricane, and an increase in surface deposit feeders from 7% pre-hurricane to 20% post-hurricane. The study highlights potential problems in interpreting post-perturbation data when insufficient pre-perturbation data exist.     

长江口及其邻近海域大型底栖动物生物量、丰度和次级生产力的初步研究

为了解长江口及邻近海域大型底栖动物生物量、丰度和次级生产力的分布情况于2004年2月、5月、8月和11月共4个航次分别在长江口40个观测站采集大型底栖动物定量样品并利用Brey的经验公式对大型底栖动物栖息丰度、生物量、次级生产力和P/B值进行了研究计算.该调查海域共采到大型底栖动物202种,其中多毛类102种,软体动物51种,甲壳类27种,棘皮动物7种,其它动物15种.大型底栖动物年平均丰度为394.7 ind/m2;年平均生物量以去灰分干重计,为2.58 g(AFDW)/m2;年平均次级生产力以去灰分干重计,为3.52 g(AFDW)/(m2*a);P/B值平均为1.53.结果表明,长江口大型底栖动物次级生产力自长江入海口向东呈递增趋势.本文分析了长江口及其邻近海域大型底栖动物优势种的组成,主要生态类群的分布特征和次级生产力分布格局与生态环境的关系.通过比较,发现长江口大型底栖动物优势种发生了较大的变化;次级生产力高于东海而低于渤海和胶州湾;P/B值高于南黄海、胶州湾和渤海,也说明了长江口大型底栖动物群落中个体小、生活史短,代谢快的种类所占的比例高于以上海域.     

渤海大型底栖动物次级生产力的初步研究

1998年 9月和 1999年 4月分别对渤海 2 0个站进行了大型底栖动物两个航次的调查取样。整个研究海域大型底栖动物年次级生产力平均值为 6 .4 9g(AFDW) /(m2 · a) ,其中渤海海峡以东海域较高 ,为 12 .5 9g(AFDW) /(m2· a) ;渤海中部为 4 .4 6 g(AFDW) /(m2· a) ,渤海大型底栖动物年平均 P/B为 0 .82     

基于多元统计分析的渔山列岛潮间带软体动物功能群和次级生产力比较

渔山列岛于2008年成为国家级海洋生态保护区,为了解渔山列岛保护区内潮间带软体动物次级生产力的时空变化。于1982年和2010年的3个季节(春、夏、冬)在渔山列岛进行潮间带软体动物调查,采用多元统计分析软体动物时空差异,以Brey经验公式计算分析软体动物次级生产力(P值,P/B值)。结果表明,年际间物种食性结构发生了明显的改变,1982年潮间带软体动物对次级生产力贡献率最大的物种有单齿螺(Monodonta labio)、锈凹螺(Chlorostoma rustica),占软体动物总次级生产力的42.88%,2010年潮间带软体动物对次级生产力贡献率最大的物种有覆瓦小蛇螺(Serpulorbis imbricata)、条纹隔贻贝(Septifer virgatus),占软体动物总次级生产力的75.96%。1982年,年均栖息密度为2758ind/m2,年均生物量为2100.26g/m2,年均次级生产力为240.04g/(m2·a),年均P/B值为0.63a–1;2010年,年均栖息密度为699ind/m2,年均生物量为1101.85g/m2,年均次级生产力为94.82g/(m2·a),年均P/B值为0.55a–1。多因素方差分析表明软体动物次级生产力年际间差异显著(F=5.761,P0.05),季节间和潮位间差异都不显著(F=0.135,P0.05;F=2.076,P0.05),P/B的值较低,表明该海域软体动物世代更替速度较慢,2010年的P/B值(0.55a–1)低于1982年的P/B值(0.63a–1),表明近30年来群落结构受到影响。     

组织培养板在获取微藻种质中的应用

报道一种基于选择演替和及时显微跟踪的原理获取一次野外水样中多种微藻的方法。利用组织培养板方便的显微观测条件和适宜容积提供微藻存活的空间,在不同培养条件下,进行微藻群落演替、及时显微跟踪检测和优势种分离,可以使野外水样初期低生物量种类优势化,以利于纯系种质的获得。并通过不同演替条件获得其中出现的未鉴定或初期样品中未检出的种质,提供进一步研究的基础。该方法在获取微藻种质和种质库建设上不失为一可行技术手段。论文以一次野外采集海水样品在不同营养条件下的主要优势种类演替跟踪检测、低生物量种质优势化过程和纯系种质分离为例,说明该方法在获取微藻种质中的应用。