点带石斑鱼人工育苗技术

通过58批次的人工育苗试验，总结点带石斑鱼人工育苗技术；阐述了育苗池、育苗的环境条件、鱼苗不同生长阶段的饵料及投喂方法、育苗水质管理及生产中的操作方法等；讨论了如何解决开口饵料问题和出现3个危险期的原因等。     

Rifting to Spreading Process along the Northern Continental Margin of the South China Sea

Understanding the development from syn-rift to spreading in the South China Sea (SCS) is important in elucidating the western Pacific's tectonic evolution because the SCS is a major tectonic constituent of the many marginal seas in the region. This paper describes research examining the transition from rifting to spreading along the northern margin of the SCS, made possible by the amalgamation of newly acquired and existing geophysical data. The northernmost SCS was surveyed as part of a joint Japan-China cooperative project (JCCP) in two phases in 1993 and 1994. The purpose of the investigation was to reveal seismic and magnetic characteristics of the transitional zone between continental crust and the abyssal basin. Compilation of marine gravity and geomagnetic data of the South China Sea clarify structural characteristics of its rifted continental and convergent margins, both past and present. Total and three component magnetic data clearly indicate the magnetic lineations of the oceanic basin and the magnetic characteristics of its varied margins. The analyses of magnetic, gravity and seismic data and other geophysical and geological information from the SCS led up to the following results: (1) N-S direction seafloor spreading started from early Eocene. There were at least four separate evolutional stages. Directions and rates of the spreading are fluctuating and unstable and spreading continued from 32 to 17 Ma. (2) The apparent difference in the present tectonism of the eastern and western parts of Continent Ocean Boundary (COB) implies that in the east of the continental breakup is governed by a strike slip faulting. (3) The seismic high velocity layer in the lower crust seems to be underplated beneath the stretched continental crust. (4) Magnetic anomaly of the continental margin area seems to be rooted in the uppermost sediment and upper part of lower crust based on the tertiary volcanism. (5) Magnetic quiet zone (MQZ) anomaly in the continental margin area coincides with COB. (6) The non-magnetic or very weakly magnetized layer is probably responsible for MQZ. One of the causes of demagnetization of the layer is due to hydrothermal alteration while high temperature mantle materials being underplated. Another explanation is that horizontal sequences of basalt each with flip-flop magnetization polarity cancel out to the resultant magnetic field on the surface. We are currently developing a synthetic database system containing datasets of seismicity, potential field data, crustal and thermal structures, and other geophysical data to facilitate the study of past, contemporary and future changes in the deep sea environment around Japan; i.e. trench, trough, subduction zones, marginal basins and island arcs. Several special characteristics are an object-oriented approach to the collection and multi-faceted studies of global data from a variety of sources.     

黄海表面水温场的准周期变化分析

本文用能谱方法分析了黄海的海表面水温(SST)场的准周期变化。由分析结果清楚地看出,SST具有高频准周期变化:黄海北部以2.6—3.2,1.4—1.7,3.6—3.8和6.7个月的准周期为主;3.3—3.6,5,1.3—2.3及6.7个月在黄海南部占优势。同时,两个海域都有6.7和3.3年的明显的准周期变化。     

Low-frequency damping of a moored semisubmersible obtained from simulated extinction tests and mean wave drift forces

Low-frequency damping of a moored semisubmersible drilling platform was obtained from numerical extinction tests simulated in still water and in regular waves and from mean wave drift forces calculated at zero forward speed. The influence of drag forces was represented by the modified Morison equation. The platform as used for the 18th ITTC Comparative Mooring Study was analyzed in irregular beam waves. The computed time series of sway as well as the corresponding sway response spectrum compared favourably with model test measurements, demonstrating that this procedure to determine low-frequency damping can be effective.     

牙鲆种群动态及其资源管理

本文根据1980—1986年间鱼类生物学参数测定,结合体长分布资料的模拟分析,研究黄、渤海区牙鲆种群年龄结构、死亡动态以及在不同年龄和死亡率条件下世代资源生物量变化的规律。模型分析结果表明,当F=1.0和t_c=6.0a时,单位补充量最大群体产量为1492g。由此认为,现阶段牙鲆资源利用的最佳策略是通过增殖放流,增加资源补充;同时应适当控制捕捞压力,减少渔具对幼鱼资源的破坏并增设繁殖保护区,以追求最大持续产量。     

鹧鸪菜组织和细胞培养的研究Ⅳ.藻体任意碎片的培养与损伤藻体再生的观察

把鹧鸪菜藻体切成大小不同的切段、以及任意切碎,培养于添加氮、磷素的消毒海水中,后者分10℃、15℃、20℃、25℃四种不同温度条件培养,结果全部切段、切碎片都很快再生出假根附着于基质,并再生出一至多个叶状体;叶状体长大后从基部长出新的假根成为完整的幼苗(再生苗)。试验表明,大的切段其再生苗生长快、长度大,但出苗率(一定长度的藻体长出再生苗数量)低。培养温度较高时(20—25℃),藻体任意切碎片再生速度快、附着速度快、数量多且较牢固。利用藻体切碎片来培养鹧鸪菜幼苗是可行的;培养的适宜温度为20℃以上、海水比重为1.010左右。本文还对鹧鸪菜损伤藻体再生的情况(繁殖生殖学)进行一些观察和描述。     

高纯河豚毒素对蛙坐骨神经动作电位的影响

用电生理学方法观察了4种不同浓度的高纯河豚毒素(tetrodotoxin,TTX)对蛙离体坐骨神经动作电位的影响.实验结果表明:1μmol/dm3TTX在给药后7 min能显著抑制动作电位的上相幅度(P<0.05),但在给药后10 min才显著抑制动作电位的下相幅度(P<0.01);20μmol/dm3TTX在给药后3 min内能完全抑制动作电位的形成.1μmol/dm3TTX在给药后1~5min对动作电位传导速度的影响没有统计学意义,5μmol/dm3TTX在5min时可显著抑制传导速度(P<0.05),而10μmol/dm3TTX在2min时便显著抑制传导速度(P<0.05).实验结果提示高纯河豚毒素对蛙离体坐骨神经动作电位的影响存在剂量效应关系.     

大黄鱼(Pseudosciaena crocea)转铁蛋白(Tf)基因克隆及序列分析

提要应用RNeasy animal mini试剂盒抽提大黄鱼total RNA,逆转录合成模板cDNA;同时综合分析从GenBank数据库查询到已报道的转铁蛋白(Tf)基因序列,先设计并合成扩增引物扩增出大黄鱼转铁蛋白部分基因序列,再应用RACE技术,克隆出大黄鱼转铁蛋白全长cDNA基因,全长2461个核苷酸,编码661个氨基酸。应用WCG软件包,采用DNADIST和NEIGHBOR分析方法,构建了转铁蛋白系统进化树。树图显示与传统的分类地位大致相符,可以看出转铁蛋白在海水鱼和淡水鱼的进化上的差异,同时也显示大黄鱼Tf与真鲷的同源性最高。     

Satellite detection of red tide in Ariake Sound, 1998–2001

High resolution SeaWiFS data was used to detect red tide events that occurred in the Ariake Sound, Japan, a small embayment known as one of the most productive areas in Japan. SeaWiFS chlorophyll data clearly showed that a large red tide event, which damaged seaweed (Nori) cultures, started early in December 2000 in Isahaya Bay, expanded to the whole sound and persisted to the end of February 2001. The monthly average of SeaWiFS data from May 1998 to December 2001 indicated that the chlorophyll peaks appeared twice a year, in early summer and in fall, after the peaks of rain and river discharge. The SeaWiFS data showed that the red tide event during 2000–2001 winter was part of the fall bloom; however, it started later and continued significantly longer than other years. Satellite ocean color data is useful to detect the red tide; however the algorithms require improvement to accurately estimate chlorophyll in highly turbid water and in red tide areas.