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51.
52.
羟化酶是细菌降解烷烃的关键酶,本文以南极低温降解菌Shewanella sp.NJ49为研究对象,通过硫酸铵沉淀、超滤浓缩及葡聚糖凝胶Sephadex G-75柱层析方法,分离纯化NJ49的羟化酶,获得了单一羟化酶组分,研究了温度、p H、表面活性剂和盐度等环境因素对NJ49羟化酶酶活活性影响。结果表明,羟化酶组分由α、β、γ三个亚基组成,分子质量分别为56,45和36 k D。NJ49最适产酶条件为:温度15℃、p H7.2、盐度55;不同类型表面活性剂对酶的活性影响效应不一,两性离子表面活性剂、阳离子表面活性剂、非离子表面活性剂对酶活没有作用,阴离子表面活性剂+非离子表面活性剂混和剂和阳离子表面活性剂+非离子表面活性剂混和剂能提高羟化酶的活性。 相似文献
53.
Guojiang?WanEmail author Jing’an?Chen Siqin?Xu Fengchang?Wu P.?H.?Santschi 《中国科学D辑(英文版)》2005,48(4):484-496
The fundamental assumption of 210Pb sediment dating is the stable flux of 210Pbex, which was derived from atmosphere and then transferred into sediments via lake water. When the sedimentation rate is relatively
constant, the 210Pbex activity in sediments will be exponentially reduced with sedimentation age. 210Pbex in lake water is incorporated into sediments mainly via organic particulates. If the sedimentation flux of organic matter
in lake water is suddenly increased, 210Pbex will be significantly deposited and then transferred into sediments. On the one hand such sudden purification effect is obviously
unfit for the fundamental assumption of 210Pb dating; on the other hand, the sudden enhancement of 210Pbex flux would be indicative of the conspicuous variation of primary productivity of lake water. This problem will be discussed
in accordance with the variation trend of 210Pbex in the vertical profile of recent sediments of Lake Chenghai, Yunnan Province. The sediment core was collected from the deep-water
area of Lake Chenghai in June 1997. The vertical profile of 137Cs activity is characterized by a tree-peak pattern. This profile gave reliable ages, and also showed the stability of sediment
accumulation in the recent ten years. The vertical profile of 210Pbex activity displays a specific distribution of peaks, and is similar to the vertical profile of Corg. This phenomenon seems to be related to the mechanism of constraining the transfer of 210Pbex into lake sediments. The average atomic ratios of Horg/Corg and Corg/Norg in Lake Chenghai sediments are 5.51 and 7.04, respectively, indicating that the organic matter was predominantly derived
from the remains of endogenic algae. In terms of the three-stage evolutionary characteristics of organic matter in sediments,
i.e., “deposition-de-composition-accumulation”, the sedimentation fluxes (F(Corg)) of organic carbon (Corg) since 1970 were calculated by modeling. The sedimentation fluxes of 210Pbex (F(210Pbex)) in different years display good synchronous relations with the sedimentation flux of organic carbon (F(Corg)), especially in the years of 1972–1974 and 1986–1989. The variation of F(Corg) led to the variation of F(210Pbex); the variation of F(210Pbex) reflects, to some extent, the historical variation of lake productivity. 相似文献
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Guanting Reservoir (GR) is one of the most important water sources for Beijing and neighboring regions. Due to water pollution, it was withdrawn from the system to supply Beijing drinking water; however, after a thorough treatment process, GR was made a reserve water source since 2007. To develop a comprehensive and quantitative analysis of water yield and purification services in the GR watershed, this study selected two time periods: the period when GR was withdrawn from the system supplying local drinking water and the period that it has been designated a reserve water source. The InVEST model was used to evaluate the quantities of water yields, and total nitrogen and total phosphorus outputs from 1995 to 2010 Additionally, the spatiotemporal variations of water yield services and water quality purification services in the GR watershed were analyzed. The results showed that water yield services in the GR watershed first weakened and then became stronger, but weakened overall during the years 1995 to 2010. Water yield capacity in the basin decreased from 1.89×109 m3 in 1995 to 1.43×109 m3 in 2010 (a drop of 24.0% in total). Water quality purification services also showed the same tendency. Total nitrogen output decreased from 4028.7 t in 1995 to 3611.4 t in 2010, while total phosphorus decreased from 379.7 t in 1995 to 354.0 t in 2010. Nitrogen and phosphorus purification services were enhanced by 10.4% and 6.8%, respectively. Changes in the climate and land use were the main factors which lead to the changes in the water yield service in the GR watershed. Policies intended to protect water resource have matched the varying trends of water quality purification services during different periods. On one hand, the research results provide a foundation to identify key fields for eco-compensation in the Guanting Reservoir basin. On another hand, the ecosystem service value will increase on the basis of eco-compensation criteria through setting the scenarios of returning farmland to forest and ecological protection. This method directly reflects increases in ecosystem service values that have occurred since measures to protect the ecological environment have been implemented. This method is more persuasive and feasible than using eco-compensation criteria based on regional ecosystem service values determined by land use/ coverage type. It can provide a new way to assess eco-compensation in the Guanting Reservoir basin and other regions. 相似文献
56.
南极因其独特的自然环境成为潜在、重要的微生物资源库,是产生新型生物活性物质和先导化合物菌株的潜在种源地,南极微生物正在成为创新药物研究新的重要资源。虽然近年来对南极微生物次级代谢产物的研究逐渐增加,但与温带和热带微生物研究相比仍处于初级阶段。对从南极普里兹湾海洋沉积物中获得的两株枝孢霉属真菌Cladosporium sp. NJF4和NJF6进行次级代谢产物分离及结构鉴定,获得20个化合物。化合物结构类型包括甾醇(1)、倍半萜类(7—8)、生物碱类(9—14)、二酮哌嗪(2—5、15—17)、芳香酸(6、18—19)等,其中倍半萜类(7—8)为首次从枝孢霉属真菌中分离得到,以上研究将为丰富南极微生物次级代谢产物库奠定一定的研究基础。 相似文献
57.
【目的】从扁舵鲣(Auxis thazard)的木瓜蛋白酶酶解产物中分离鉴定具有较高1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除率的抗氧化肽。【方法】用木瓜蛋白酶水解扁舵鲣,以DPPH自由基清除能力为检测指标,通过超滤、凝胶过滤层析和反向高效液相色谱分离抗氧化肽,再经过超高效液相/三重四级杆飞行时间质谱(UPLC/Xevo G2-XS QTOF)进行结构鉴定。【结果】从酶解产物中获得3种抗氧化肽,其氨基酸序列分别为β-丙氨酸-1-甲基-L-组氨酸(241 u)、Gly-Ala-Gly-Gly-Pro(357 u)和Val-Glu(246 u)。【结论】扁舵鲣的木瓜蛋白酶酶解产物含有抗氧化活性的肽类,可为其抗氧化肽的开发提供理论依据。 相似文献
58.
城市污水处理厂污泥利用现状及潜在环境问题 总被引:9,自引:0,他引:9
根据国家环保总局要求,在2010年前全国主要城市的污水处理率要达到70%,这将使我国的水环境得到明显改善,另一方面也带来了污泥的处置和利用问题.污泥是一种优质的有机肥料,有选择地控制使用,既可满足作物生长对养分的需要,又可培育土壤.符合污泥固废“减量化”和“资源化”的处置要求. 相似文献
59.
东营凹陷古近系古湖盆演化与水化学场响应 总被引:10,自引:2,他引:10
采用人工配制污水为实验用水进行室内水培实验,研究10种人工湿地中常见水生植物对氨氮和总磷的同化吸收能力,筛选出净化效果好的植物,构建植物组合,以单一植物为对照,研究植物组合对于提高氨氮、总磷净化效果的作用.结果表明:不同植物对氨氮、总磷的去除能力差别较大千屈菜(Lythrum salicaria)、菖蒲(Acorus calamus)、美人蕉(Cannindica)、达香蒲(Typha davidiana)对氨氮净化效果较好,初始浓度2969mg/L、15d后,去除率966%、98.6%、877%、95.1%美人蕉、凤眼莲(Eichhornia crssipes)、干屈菜、石菖蒲(Acorus tartarinowii)对TP净化效果较好,初始浓度4.44mg/L、15d后,去除率99.0%、546%、69.9%、36.7%千屈菜与石菖蒲的组合能同时提高氨氮与总磷的净化效果;美人蕉与干屈菜的组合、菖蒲与美人蕉的组合、菖蒲与千屈菜的组合、美人蕉与石菖蒲的组合能提高总磷的净化效果组合实验结果表明,适当的水生植物组合能提高氨氮与总磷的净化效果。 相似文献
60.