Production and transport of long-chain alkenones and alkyl alkenoates in a sea water column in the northwestern Pacific off central Japan

Sinking particles collected from year-long time-series sediment traps at 1674, 4180, 5687 and 8688 m depths, the underlying bottom sediment at 9200 m depth, and suspended particles from surface and subsurface waters in the northwestern North Pacific off Japan were analyzed for long-chain alkenones and alkyl alkenoates (A&A) which are derived mainly from Gephyrocapsacean algae, especially Emiliania huxleyi and Gephyrocapsa oceanica. Alkenone temperature records in sediment trap samples at 1674 m were almost similar to observed sea surface temperatures (SST) with a time delay of one half to one full month. However, alkenone temperatures in trap samples were about slightly lower than measured SST in late spring to early fall. The lowering might be caused by formation of the seasonal thermocline. Nevertheless, these temperature drops observed in trap samples were smaller than those actually observed in a subsurface layer off central Japan. Vertical profiles of A&A concentrations and alkenone temperatures in suspended particles collected from the subsurface waters in early fall indicated that these compounds were produced mostly in a surface mixed layer above the depth of the chlorophyll maximum even in warm seasons. These results suggested that alkenone temperatures strongly reflected SST rather than the temperatures of thermocline waters in these study areas even in such a warm season. Pronounced maxima in A&A fluxes found in sediment trap samples at 1674 m in late spring to summer showed that A&A productions were highest during the periods of spring bloom, according to a time delay between alkenone temperatures and observed SST. Seasonal patterns of alkenone records in trap samples at 4180 and 5687 m could also preserve SST signals well, suggesting that A&A in deep sea waters were mainly derived from primary products in the surface layer. A&A fluxes tended to decrease with water depth, and the ratios of A&A to particulate organic carbon (POC) rapidly decreased in underlying bottom sediment. This clearly indicates that A&A were decomposed and diluted by other refractory organic materials in either the water column or the sediment–water interface. However, A&A compositions were consistently uniform between the trap samples and the underlying bottom sediments, so that A&A could not qualitatively alter during early diagenetic processes.     

摄食对中国对虾能量代谢影响的初步研究

于 1996年 5～ 6月在水温 2 5℃时测定了两种规格中国对虾 ( Penaeus chinensis)摄食沙蚕和配合饲料后的耗养率和氨排泄率。小规格中国对虾摄食配饵的代谢率略高于摄食沙蚕 ,而大规格组中摄食沙蚕的代谢率大于摄食配饵组。小规格中国对虾摄食配饵和沙蚕的代谢能比未摄食组提高了 33.2 2 %和 2 7.4 0 %;大规格中国对虾摄食配饵和沙蚕的代谢能比未摄食组提高了 34 .78%和 4 0 .0 5%。中国对虾摄食后的氨排泄率明显增加 ,表明蛋白质在摄食代谢中具有重要作用。     

利用酵母双杂交研究铜绿微囊藻生物钟蛋白KaiB的自身相互作用

从铜绿微囊藻(Microcystis aeruginosa)PCC7820中克隆了生物钟基因kaiB,并将其克隆入酵母双杂交系统的两个质粒pGADT7和pGBKT7中。经测序验证后,将重组质粒pGADT7-kaiB和pGBKT7-kaiB转化酵母菌AH109进行自激活性和自身相互作用的检测和验证。结果表明,铜绿微囊藻生物钟蛋白KaiB无自激活性,KaiB蛋白自身能发生相互作用。因此,可用KaiB蛋白为诱饵利用酵母双杂交系统筛选文库钓取与KaiB发生相互作用的蛋白。     

海水升温对非虫黄藻共生型珊瑚Cladopsammia sp.的生理影响

文章以非虫黄藻共生型珊瑚Cladopsammia sp.为研究对象,将对照组的海水设置为26℃恒温,试验组从26℃升温至33℃,以此探究海水升温对Cladopsammia sp.代谢和钙化生理的影响。结果显示:在高温胁迫下,虽然Cladopsammia sp.钙化相关酶(Ca²⁺-ATP酶和Mg²⁺-ATP酶)的活性出现了负响应,但是珊瑚的呼吸速率、生长速率、组织中总蛋白和粗脂肪都没有显著改变(p>0.05)。比较历史文献结果和本研究结果表明:Cladopsammiasp.可能由于体内没有虫黄藻共生,表现出了比大部分虫黄藻共生珊瑚更高的温度耐受能力。     

Seasonal variation of the glycogen enzyme activity in diploid and triploid Pacific oyster gonad during sexual maturation

The glycogen content and the activities of two key enzymes in glycogen metabolism, glycogen phosphorylase and gly- cogen synthetase, in the gonad of diploid and triploid Pacific oysters (Crassostrea gigas) were compared during maturation. The glycogen content in the gonad of diploids decreased with gametogenesis (by 85.7%), but the glycogen content in the gonad of trip- loids did not vary significantly. Activity of glycogen phosphorylase (GP) in the gonad of diploids decreased with gametogenesis (by 55.5%), while GP activity of triploids did not vary significantly during maturation. Activity of glycogen synthetase (GS) in the gonad of diploids increased slightly with gametogenesis, reaching a peak in June. Activity of GS declined sharply from June to July, which might be due to gonad spawning. GS activity of triploid oysters in spawning time (July and August) was significantly higher than that in other months, which might be explained with a ‘compensating’ mechanism for the higher glycogen content in triploids.     

孔雀石绿的代谢机理及生物毒性研究进展

孔雀石绿(Malachite green,MG)是1种三苯甲烷类工业染料,曾广泛用于治疗水产动物的水霉病、烂鳃病、寄生虫病等,但由于具有高毒、高残留、“三致”等毒性性质,许多国家禁止其在经济鱼类(观赏鱼除外)的养殖过程中使用,成为药物残留监控的主要内容之一。本文对孔雀石绿的化学性质、代谢规律及机理、生物毒性等研究现状进行了综述,旨在为药残的监控工作提供理论依据。     

生态足迹评介及国际研究前沿

生态足迹评价方法是评估可持续发展的一个全球可比的、可测度的直观且综合的指标,有明显的政策含义,是迄今提出的评估可持续发展的众多指标中最受生态经济学界关注、推崇和广泛应用的一个指标。评介了生态足迹方法模型的优缺点,综述了国际上生态足迹研究的最新进展及研究前沿,指出了国内围绕生态足迹研究应该尽快开展的工作：①生态足迹方法模型的改进及应用研究;②丰富生态足迹方法模型携带的信息量和政策内涵;③社会经济系统新陈代谢以及人类对初级生产力的占用研究;④消费模式与全球资源和环境的压力的关系;⑤物质流核算帐户研究和生态包袱分析研究。     

褐藻酸降解菌的生长条件及其对海带的生理效应

为更好地了解海带病烂的发生机制,针对褐藻酸降解菌埃氏交替单胞菌（Altermonas espejiana）菌株A1的生长及产酶条件进行了初步研究,并用不同浓度的菌液对健康海带进行了感染试验,测定了其体内可溶性蛋白质和可溶性糖含量的变化。结果表明,该菌株生长和产酶的最适条件为：20℃,0．5％～0．6％褐藻酸钠,pH=7,5,氮源为（NH4）2S04,培养时间72h。此外,感染菌株A1后,海带体内可溶性蛋白质和可溶性糖的含量均增加,且随着感染菌液浓度的升高,海带体内可溶性蛋白质含量升高,而可溶性糖含量先升高后降低。     

城市新陈代谢视角下的城市公共健康风险

城市公共健康风险是当代城市研究的一个重要主题,在中国人文地理学界尚未得到充分关注。国内既有研究主要集中在两个维度——健康城市建设和城市风险治理,然而这些研究受到城市/自然二元论的影响。自20世纪90年代以来,城市政治生态学在西方人文地理学界迅速崛起,提出了以社会关系为核心的城市新陈代谢理论,在空间和联系两个层面将社会与自然,人类行为体与非人类行为体联系起来,实现了对二元论的超越。基于这一理论,本文从空间和联系层面对城市公共健康风险的具体形式进行分析,指出在空间层面上,存在空间流转和重叠的公共健康风险和空间生产和再生产活动的公共健康风险;就联系层面而言,存在“新陈代谢断裂”的公共健康风险和公众认知的公共健康风险。