鲐鱼灯光围网渔船水上集鱼灯水中照度分布及优化配置的理论计算

采用叠加法照度计算模式,根据一组鲐鱼灯光围网渔船中3艘不同渔船的水上集鱼灯布置参数,使用自行开发的水上集鱼灯水下光场计算系统,对各船的水中照度分布进行了计算,获得船舷右侧中部水深方向200 m范围内不同深度的照度,并使用Surfer 8.0软件绘制等值曲线图,结果表明:主灯船在总功率为180 kW的情况下,10 lx的等照度曲线水平方向最远在56 m左右,水深方向不超过20 m;离船水平距离40 m、垂直距离40 m处的照度约为0.01 lx。副灯船在总功率为120 kW的情况下,10 lx的等照度曲线水平方向最远在46 m左右,水深方向不超过18 m;离船水平距离33 m、垂直距离38 m处的照度约为0.01 lx。网船在总功率为40 kW的情况下,10 lx的等照度曲线水平方向最远在45 m左右,水深方向不超过15 m;离船水平距离30 m、垂直距离35 m处的照度为0.01 lx左右。从集鱼灯的配置情况来看,主灯船、副灯船与网船目前的配置均较好,但主灯船在灯距增大到0.26 m、灯高增大到5 m时可增加1.6%的有效水体体积,副灯船在灯距减少到0.28 m、灯高增大到5 m时可增加2.1%的有效水体体积,网船在灯距增大到0.64 m、灯高增大到9 m时可增加1.7%的有效水体体积。计算结果还表明,大幅度的增加光诱渔船的集鱼灯功率并不能很有效的提高该船的光诱范围。     

祁连山老虎沟12号冰川退缩对细菌优势种群影响的初步研究

随着全球冰川正在越来越多的地区融化, 冰川微生物资源很可能会由于冰川退缩而未被人类所发现就已受到生存的威胁而濒危.以祁连山老虎沟12号冰川消融区和末端雪样及末端土样为研究对象, 采用培养方法、分子鉴定, 研究冰川雪样优势菌群在冰川末端土样的分布状况及生理生化指标变化情况, 分析冰川细菌优势菌群在冰川退缩后适应非雪环境的能力.结果表明: 冰川末端雪样优势菌为1BW1和1BW2所代表的Pedobacter, 该属在冰川消融区雪样和冰川末端土样中未分离到; 冰川消融区雪样优势菌为2BW所代表的Acinetobacter, 该属在冰川末端雪样中的数量较少, 在冰川末端土样中的数量更少.不同采样位点16S rRNA序列相似性高的菌株其生理生化特征比相同采样位点的大.因此, 冰川冰退缩可能会引发冰川雪样中的优势种群不能适应新环境而灭绝. 应加强冰川细菌资源利用和保护的研究基础.     

Population genetics of Sargassum horneri (Fucales, Phaeophyta) in China revealed by ISSR and SRAP markers

Sargassum horneri is a common brown macro-alga that is found in the inter-tidal ecosystems of China. To investigate the current status of seaweed resources and provide basic data for its sustainable development, ISSR (inter simple sequence repeat) and SRAP (sequence related amplified polymorphism) markers were used to analyze the population genetics among nine natural populations of S. horneri. The nine studied populations were distributed over 2 000 km from northeast to south China. The percentage of polymorphic loci P% (ISSR, 99.44%; SRAP, 100.00%), Nei’s genetic diversity H (ISSR, 0.107-0.199; SRAP, 0.100-0.153), and Shannon’s information index I (ISSR, 0.157-0.291; SRAP, 0.148-0.219) indicated a fair amount of genetic variability among the nine populations. Moreover, the high degree of gene differentiation G st (ISSR, 0.654; SRAP, 0.718) and low gene flow N m (ISSR, 0.265; SRAP, 0.196) implied that there was significant among-population differentiation, possibly as a result of habitat fragmentation. The matrices of genetic distances and fixation indices (F st ) among the populations correlated well with their geographical distribution (Mantel test R=0.541 5, 0.541 8; P=0.005 0, 0.002 0 and R=0.728 6, 0.641 2; P=0.001 0, 0.001 0, respectively); the Rongcheng population in the Shandong peninsula was the only exception. Overall, the genetic differentiation agreed with the geographic isolation. The fair amount of genetic diversity that was revealed in the S. horneri populations in China indicated that the seaweed resources had not been seriously affected by external factors.     

Genetic diversity and population structure of Eleutheronema rhadinum in the East and South China Seas revealed in mitochondrial COI sequences

Eleutheronema rhadinum is a potential commercial fisheries species and is subject to intense exploitation in China. Knowledge on the population structure of E. rhadinum in Chinese coastal waters, which is important for sustainable exploitation and proper resource management, is lacking. In the present study, the genetic diversity and population structure of E. rhadinum were evaluated using a 564-base pair fragment of the mitochondrial cytochrome c oxidase subunit I （COl） gene. A total of 76 specimens were collected from three localities around the East （Qidong and Zhoushan） and South China Seas （Zhuhai）. Among these individuals, nine polymorphic sites were detected and 1 l distinct haplotypes were defined. High levels ofhaplotype diversity （h=0.759i0.035） and low levels of nucleotide diversity （re=0.001 98i0.003 26） were observed in these populations, Hierarchical analysis of molecular variance （AMOVA） indicated that 96.72% of the genetic variation occurred within the populations, whereas 3.28% occurred among populations. No significant genealogical branches or clusters were recognized on the neighbor-joining tree. Intra-group variation among populations was significant （~0~t=0.032 85, P〈0.01）. These results suggest that E. rhadinum populations in the East and South China Seas have developed divergent genetic structures. Tests of neutral evolution and mismatch distribution suggest that E. rhadinum may have experienced a population expansion. The present study provides basic information for the conservation and sustainable exploitation of this species.     

面向防震减灾的人口数据空间化研究——以2007年宁洱地震灾区为例

入口是地震灾害的重要受灾体,准确的入口空问分布信息是防震减灾工作的重要依据.本文借助地理信息系统,将人口统计数据与高分辨率遥感数据相结合,应用基于居民地的人口数据空间化方法,模拟人口空间分布.首先根据城市人口—面积异速生长模型的分形几何意义,推导出城乡人口一面积统一模型;进而以2007年宁洱地震灾区为例,在建立居民地分类体系和遥感解译标志的基础上,目视解译获得准确的居民地信息;最后应用城乡人口—面积统一模型获得网格人口密度矢量数据.经检验,本文的结果达到了较高的精度.同时在人口数据空间化完成的基础上,以地震受灾人口估算为例,探讨了人口数据空间化在防震减灾中的应用.研究结果表明,基于网格人口矢量数据的受灾人口估算结果更能客观反映地震灾情,可以为防震减灾和应急救援工作提供可靠的依据.     

Evidence of the restricted gene flow within a small spatial scale in the Japanese common intertidal goby Chaenogobius annularis

Elucidating the scale of gene flow among populations is an important challenge for understanding the ecological dynamics and local adaptation of marine organisms. We assessed whether gene flow is restricted even at a small spatial scale in the Japanese common intertidal goby Chaenogobius annularis, using highly polymorphic DNA markers, involving the mitochondrial DNA (mtDNA) control region and 15 microsatellite DNA (msDNA), because past ecological studies have suggested low dispersal ability for rocky intertidal fishes. We found significant heterogeneities between four neighboring local populations by both mtDNA and msDNA analyses. In addition, no genetic heterogeneity was detected by either method across generations within a population; it was considered that such genetic differentiation is retained across generations and that the gene flow of this species is restricted to within a radius of a few kilometers. This is the first report showing a clear genetic subdivision in rocky intertidal fish.     

Changes in vertical distribution and aggregative behaviour in response to population density for larval sea urchins (Strongylocentrotus droebachiensis) and sea stars (Asterias rubens)

We examined the effects of the presence of larval conspecifics on larval vertical distribution of four‐arm and six‐arm plutei of Strongylocentrotus droebachiensis and bipinnaria of Asterias rubens, in laboratory experiments, by manipulating population density. Larvae were introduced to experimental columns (10 × 10 × 30 cm) at one of two or three population densities (S. droebachiensis: 0.1 and 10 larvae·ml^?1; A. rubens: 0.1, 1 and 10 larvae·ml^?1). Subsequent changes in vertical distribution were determined from images of the larvae in the columns illuminated by a 532‐nm laser and captured by a high‐resolution camera. Larvae of both species were found higher in the water column in the high than in the low and intermediate population densities. The relationship between vertical swimming velocity and nearest neighbour distance (NND) was measured for four‐arm plutei of S. droebachiensis, and used to determine a range in distances among individuals that may affect potential interactions. The variation in swimming velocity decreased with increasing proximity below a threshold distance among individuals of 10 mm, which was greater than the estimated distance in the high population density. We suggest that the increased larval aggregation near the water surface under high population density is the result of a behavioural response to conspecifics.     

Genetic variability of the blue and red shrimp Aristeus antennatus in the Western Mediterranean Sea inferred by DNA microsatellite loci

Genetic variation at eight microsatellite loci was studied in nine populations of the blue and red shrimp Aristeus antennatus to investigate whether distinct stocks are present in the Western Mediterranean Sea. A high level of gene flow and no evidence of genetic partitioning were discovered. No significant variation was found (F_ST = 0.00673, P-value = 0.067) even when shrimps from exploited and those from deep-water unexploited grounds were compared. No evidence of reduction or expansion of population size in the recent past was found, as indicated by the bottleneck and interlocus g-tests. Our results are consistent with previous studies using mitochondrial gene methods and allozymes, indicating that, for this species, extensive pelagic larval dispersal and adult migration are probably responsible for the genetic homogeneity observed. In particular, due to a different bathymetric distribution of males and females, reported to be associated with different water masses and hence with possible differential dispersal capacity between sexes, the hypothesis of sex-biased dispersal was tested. Mean values of corrected assignment indices and mean relatedness values were higher for males, suggesting that females are the more widely dispersing sex. Molecular assessment of A. antennatus from the Western Mediterranean provides data of biological and evolutionary interest for the successful management of such a highly valuable fishery resource.     

海洋围隔生态系中浮游动物的研究

2002-05-20-26在东海进行小尺度船基浮游生物生态系围隔实验,研究浮游动物的群落结构、组成与环境之间的关系,探讨了优势种群的自然繁殖发育期。结果表明,浮游动物的种类组成较简单,优势种明显。浮游动物生物量、丰度及优势种群丰度三者的变化呈逐渐增加趋势。在实验期的1～5d,小拟哲水蚤的无节幼虫和早期幼体占绝对优势,该自然种群的丰度不断增加;随时间推移,无节幼虫逐渐发育为桡足幼体,而后又逐渐发育为成体。因此,本次实验期是实验海区优势种小拟哲水蚤种群的自然繁殖发育盛期。     

中国省域犯罪率影响因素的空间非平稳性分析

收入差距和流动人口是影响犯罪率的两个重要因素, 以往研究基于OLS模型, 在假设地域空间为均质的前提下分析其对犯罪率的影响, 但现实世界的空间单元往往难以满足“均质”的假设, 多数表现为“空间异质”。以OLS计量空间异质会造成计量结果出现偏差, 同时无法了解不同空间单元的不同影响。而地理加权回归模型通过将空间结构嵌入线性回归模型中, 很好的解决了空间异质的计量问题。利用地理加权回归模型研究2008 年中国大陆省域单元犯罪率的影响因素, 结果表明:① 犯罪率的影响因素表现出空间非平稳性, 流动人口与犯罪率显著相关, 但各个省份相关程度并不相同, 影响关系随空间位置变化而变化;② 地理加权回归模型的计量精度和拟合度比OLS模型有大幅提高