Bacterial production,glucosidase activity and particle-associated carbohydrates in Dona Paula bay,west coast of India

Size-fractionated bacterial production, abundance and α- and β- glucosidase enzyme activities were studied with respect to changes in hydrography, total suspended matter (TSM), chlorophyll a, particulate organic carbon and nitrogen ratio (POC:PON), 1.5 M NaCl-soluble and 10 mM EDTA-soluble carbohydrates (Sal-PCHO and CPCHO) and transparent exopolymeric particles (TEP) in the surface waters from July 1999–2000 at a shallow coastal station in Dona Paula Bay, west coast of India. The bulk of the total bacterial production and glucosidase activity were associated with particles (75% and >80%, respectively). Total bacterial production was linearly correlated to chlorophyll a (r = 0.513; p < 0.05) whereas enzyme activity was significantly correlated to TSM (α-glucosidase: r = 0.721 (p < 0.001); β-glucosidase: r = 0.596 (p < 0.01)). Both α-glucosidase (r = 0.514; p < 0.05) and β-glucosidase enzymes (r = 0.598; p < 0.01) appeared to be involved in the degradation of CPCHO and Sal-PCHO, respectively. Changes in α-glucosidase/β-glucosidase ratios highlighted the varying composition of particulate organic matter. The bacterial uptake of ¹⁴C-labeled bacterial extracellular carbohydrate measured over 11 days showed a strong linear correlation between ¹⁴C-uptake and bacterial production using tritiated thymidine. The turnover rate of ¹⁴C-labeled carbohydrate-C was 0.52 d⁻¹, higher than the estimated annual mean potential carbohydrate carbon turnover rate of 0.33 ± 0.2 d⁻¹. Our study suggests that carbohydrates derived from sediments may serve as an important alternative carbon source sustaining the bacterial carbon demand in the surface waters of Dona Paula Bay.     

霸王根茎叶非结构性碳与C:N:P计量特征对干旱的响应

通过选取阿拉善地区3个典型霸王群落为研究对象,研究霸王器官间(根、茎、叶)生态化学计量比特征及非结构性碳水化合物(Non-structural carbohydrates,NSCs)的变化,以便加深对霸王生存策略的理解,更好的服务于荒漠生态系统的生态恢复.结果 表明:霸王茎和叶中可溶性糖(Solu?ble sugar,...     

Distribution and seasonal variation of concentrations of particulate carbohydrates and uronic acids in the northern Indian Ocean

Suspended particulate matter (SPM) samples were collected from the surface seawaters at 31 stations, and from various depths (2 to 1000 m) at 9 locations in the northern Indian Ocean during various seasons. SPM samples were analyzed for total particulate carbohydrate (TPCHO), total particulate uronic acid (TPURA) and total particulate neutral carbohydrate (TPNCHO) concentrations and composition. Strong spatial, temporal and depth related variations were evident in the distribution of these compounds. In surface waters, concentrations of TPCHO, TPNCHO, and TPURA varied from 0.57 to 3.58 μM C, 0.11 to 2.34 μM C, and from 0.01 to 0.31 μM C, respectively, and accounted for 2.6 to 34.6%, 2 to 24.5%, and 0.2 to 6.3% of POC, respectively, whereas the TPURA accounted for 4.7 to 22.7% of TPCHO. Concentrations and yields of both TPNCHO and TPURA decreased rapidly in the upper 100 m of the water column suggesting their utilization by heterotrophic organisms. Glucose was the most abundant constituent of the TPNCHO. Glucose mole fraction decreased while that of other monosaccharides, especially galactose, arabinose, mannose, rhamnose and fucose increased in the upper 100 m water. Below this depth, mole fraction of glucose increased while that of other sugars decreased with the increasing water depth. Generally, high C:N ratios were associated with low yields of carbohydrates and uronic acids. Inverse correlation between the mole fractions of arabinose plus xylose and rhamnose plus fucose indicates the importance of biogenic and terrestrial organic matter input to the Bay of Bengal. TPURA are surface-active in nature and thus may play an important role in coagulation of particles and macromolecules. The observed spatial and seasonal variations of these compounds may be due to differences in phytoplankton biomass, nutrient status, and the influence of terrestrial material.     

Seasonal Changes in Quality and Quantity of Food Available for Benthic Suspension-feeders in the Golfo Marconi (North-western Mediterranean)

Suspended particulate matter was collected, from the water layer at 10 cm above the sediments, over a period of 13 months in the Golfo Marconi (Ligurian Sea, NW Mediterranean). Measurements of seston concentration as well as the elemental (particulate organic carbon and nitrogen; POC and PON, respectively) and biochemical composition (lipids, proteins, carbohydrates, DNA) of particulate organic matter were carried out to assess quality and quantity of food potentially available to benthic suspension-feeders. Particulate organic matter showed wide qualitative and quantitative variations during the sampling year. Seston concentrations and POC did not reflect the quantity and quality of the food available to benthic suspension-feeders. The biopolymeric fraction of particulate matter (C-BPF, i.e. the sum of lipid, protein and carbohydrate carbon) was mostly composed of phytoplankton (which accounted for about 60% of C-BPF). The ratio of C-BPF to POC was utilized as a measure of the fraction which had the potential to be more readily available to consumers. Suspended organic matter showed higher values of the C-BPF:POC ratio during spring, and lower values in summer and autumn–winter. Quantitative estimates of the energy content of the suspended particulate matter were obtained from its biochemical composition. Bacterial dynamics were significantly related to changes in phytoplankton biomass. Bacteria accounted for a significant fraction of the biopolymeric carbon pool (annual average about 15%) and of the total particulate DNA (21·5%), thus enhancing the nutritional value of the particulate organic matter. The results achieved in this study indicate that the biochemical composition of the particulate matter provides additional information on the origin, quality and characteristics of the seston more readily available to benthic suspension-feeders.     

Concentration, Molecular Weight Distribution and Neutral Sugar Composition of DOC in Maritime Antarctic Lakes of Differing Trophic Status

The molecular weight distributions and hydrolysable neutral sugar composition of dissolved organic carbon (DOC) was investigated in four maritime Antarctic lakes on Signy Island of different trophic status; Heywood Lake (eutrophic), Light Lake (oligo-mesotrophic), Sombre Lake and Moss Lake (both oligotrophic). Tangential flow ultra-filtration (TFU) was used to separate DOC into high molecular weight (HMW; >1000 Da) and low molecular weight (LMW; < 1000 Da) size fractions. Pulsed amperometric detection-high performance liquid chromatography (PAD-HPLC) was used to determine the hydrolysable neutral sugar molecular composition of each size fraction. Total DOC concentrations defined the trophic trend in the four lakes and ranged from 8 to 303 μM. The <1000 Da fraction of all the lakes dominated the DOC distribution, comprising 76% in Light Lake which also had the highest chl-a concentrations. Heywood Lake was relatively enriched in >1000 Da total organic carbon and had extremely high concentrations of total hydrolysable neutral sugars (11 μM) corresponding to 43% of total DOC. However, no clear pattern was apparent with regard to lake trophic status and potential sources of DOC, and the measured variations in individual aldose concentration, composition and their various molecular weight fractions.     

Establishing the Database of Inundation Potential in Taiwan

The structural measure was the major solution for flood defense in Taiwan. However, the measure is always limited to the design standard and cannot prevent the damages when floods exceed certain scale. Therefore, non-structural measures for flood mitigation are the indispensable complements to structural solutions. The study introduces the establishment of inundation potential database that provides required information for the non-structural measures in Taiwan. The database was built by numerical simulations, based on different rainfall scenarios, and has been applied by the local governments of Taiwan for land use managements, flood warning systems, emergency responses, and flood insurance programs to reduce the flood damages and impacts.     

Chemical characterization and composition of dissolved organic matter in Jiaozhou Bay

Biologically utilizable dissolved organic compounds, including dissolved organic carbon (DOC), dissolved carbohydrates (DCHO) and dissolved free amino acids (DFAA) were analyzed in filtered surface seawater samples collected at 19 stations in Jiaozhou Bay, China, on June 3, 2007. In these samples, concentrations of DOC, dissolved free carbohydrates (DFCHO), dissolved combined carbohydrates (DCCHO), total dissolved carbohydrates (TDCHO) and total dissolved free amino acids (TDFAA) ranged from 141.7 to 191.1 μmol C/L, 1.98 to 18.18 μmol C/L, 5.04 to 24.90 μmol C/L, 14.52 to 30.36 μmol C/L, and 1.83 to 11.89 μmol C/L, respectively. As a major component of the dissolved carbohydrates, the concentrations of DCCHO were about three times higher than those of DFCHO. Three major constituents of the DFAA were threonine (23.0±5.7 mol%), glutamic acid (16.6±3.2 mol%) and arginine (9.1±3.3 mol%). Based on the composition of DFAA, a molar C:N ratio of 3.60±0.75 in DFAA was derived, indicating longer carbon chains in the amino acids. DCCHO (8.1%) was the most abundant fraction of DOM in most samples, followed by DFCHO (4.8%) and TDFAA (2.7%). These DOM concentrations displayed a decreasing trend from the coast to the central region. Significant correlations were found between the DCCHO and DFCHO concentrations (r=-0.724, n=19, P<0.001) and the DCCHO and TDCHO concentrations (r=0.506, n=19, P=0.027).     

考虑非结构构件损伤的钢筋混凝土框架建筑多维地震易损性分析

基于单一指标的传统地震易损性分析忽略了非结构构件损伤对建筑抗震性能的影响。首先基于多维性能极限状态理论建立了三维性能极限状态方程,并对几种特殊情况下的三维阈值曲面进行了讨论。进而以最大层间位移角作为整体结构与位移敏感型非结构构件的性能指标,以峰值楼面加速度作为加速度敏感型非结构构件的性能指标,对建筑的结构损伤和非结构损伤进行描述。考虑各性能指标之间的相关性和各性能指标所对应的极限状态阈值的不确定性,建立了建筑在地震作用下的三维性能极限状态的超越概率函数。最后,采用Open Sees有限元软件对一7层钢筋混凝土框架填充墙建筑进行增量动力分析,得到其各性能水平下的地震易损性曲线。分析结果表明,当忽略非结构构件损伤时,各性能极限状态的超越概率均降低,从而高估了建筑剩余功能水平,进而导致低估建筑的损失。在考虑各性能指标的极限状态阈值的不确定性时,对任一性能极限状态,不同变异系数取值下的易损性曲线会出现交点,在交点之前超越概率随着变异系数的增大而增大,交点之后则随着变异系数的增大而减小。在考虑性能指标间的相关性时,对任一性能极限状态,超越概率随着相关系数的减小而增大。另外,性能指标阈值的不确定性与性能指标间的相关性对地震易损性的影响随着性能水平的提高而逐渐降低,且对低性能水平下建筑地震易损性有明显影响。