响应面法优化热泵-热风联合干燥小黄鱼(Pseudosciaena polyactis)的节能参数

为了减少小黄鱼(Pseudosciaena polyactis)干燥过程的能耗, 在前期实验的基础上, 采用响应面分析法, 以单位能耗除湿值(SMER)和挥发性盐基总氮值(T-VBN)两者的综合指标Y为最终评价指标, 对热泵干燥阶段的温度A、分阶段干燥的转换点含水率B、后期热风干燥阶段的温度C三个条件进行优化, 模拟得到回归方程的预估模型。结果表明, 小黄鱼联合干燥的最优条件为热泵干燥温度A=50℃、转换点含水率B=32.62%和热风干燥温度C=44.01℃, 在此条件下比较热泵干燥(HPD)、热泵-热风联合干燥(HPD+AD)、热风干燥(AD)三种干燥方式下的样品品质和耗能, 联合干燥得到的小黄鱼干品质高于热风干燥, 而且干燥能耗降低了34.8%。     

牙鲆(Paralichthys olivaceus)β诺达病毒衣壳蛋白重组表达及复性条件优化 组表达及复性条件优化

β诺达病毒利用其缺乏校正功能的聚合酶进行基因组复制, 易导致突变, 因而具有广泛的宿主感染性, 而且可引发致死率极高的病毒性神经坏死症, 需要有针对性地研究检测及防御方法。本研究以感染牙鲆(Paralichthys olivaceus)的β诺达病毒为对象, 利用引物设计, 将His标签编码序列连接到完整病毒衣壳蛋白C端, 构建原核表达载体; 采用SDS-PAGE及质谱对表达产物进行分离和鉴定; 重组衣壳蛋白经镍离子亲和柱纯化后进行复性条件的正交优化。结果表明4个肽段经质谱鉴 定与预期一致; 纯化产物产量可达36mg/L; 4°C条件下, 复性缓冲液中尿素和PBS的最适浓度为0.8mol/L和0.05mol/L。本研究建立的制备方案可为研制相关疫苗以及开发针对该病毒的快速检测产品等提供有价值的参考。     

基于稀疏AR模型的潮流信号建模与预报

潮流信号处理与预报在很多方面具有非常重要的意义和价值。本文引入信号稀疏表示理论,构建一种稀疏AR模型,寻找各潮流数据间的历史关联性,并进行预报分析。首先由实测潮流信号进行常规AR建模,获得一组过完备稀疏基;其次随机从该过完备稀疏基抽取部分建立欠定方程组,利用稀疏优化算法获得最稀疏的AR系数;多次重复上一步,获得稀疏AR系数的平均以增强稀疏AR模型的稳定性;最后利用这些稀疏AR系数来重构或预测潮流信号。文章针对实测潮流信号,特别是存在多峰值有回流现象的潮流信号,进行了稀疏AR建模与预测的多次实验。实验结果与传统的潮流信号调和预报方法相对比,发现基于稀疏AR模型的潮流预报对于潮流存在多变的现象时,具有明显优越性,从回报结果来看,稀疏AR模型的潮流预报均方差明显小于传统潮流调和分析预报方法。     

基于四种水产病原弧菌的简型芯片检测体系的优化

基因芯片技术在病原菌检测领域展现出良好的前景,本文依托水产病原菌基因检测芯片的检测体系,以水产常见病原弧菌的hsp60、toxR基因检测探针为研究对象,系统研究不同点样液配方、寡核苷酸探针浓度、荧光标记物质浓度、杂交温度、杂交时长对芯片杂交效果的影响,并进行相应优化。结果表明,杂交温度为60℃、杂交时长为2h、探针浓度为1μmol/L、Cy3标记的随机引物的用量为150pmol时,芯片的经济性、灵敏度、检测效率达到均衡;以0.1mol/L磷酸氢二钠和25%二甲基亚砜混合溶液作为点样液,效果较好。优化后的芯片对4种弧菌的检测没有出现交叉杂交现象,探针特异性达到100%;对溶藻弧菌全基因组DNA检测灵敏度为10fg,高出PCR方法约2个数量级。     

直驱式波浪能转换系统的研究和性能优化

A direct-drive wave energy conversion system based on a three-phase permanent magnet tubular linear generator （PMTLG） and a heaving buoy is proposed to convert wave energy into electrical energy. Sufficient experimental methods are adopted to compare the computer simulations, the validity of which is verified by the experiment results from a wave tank laboratory. In the experiment, the motion curves of heaving buoy are with small fluctuations, mainly caused by the PMTLG＇s detent force. For the reduction of these small fluctuations and a maximum operational efficiency of the direct-drive wave energy conversion system, the PMTLG＇s detent force minimization technique and the heaving buoy optimization will be discussed. It is discovered that the operational efficiency of the direct-drive wave energy conversion system increases dramatically after optimization. The experiment and optimization results will provide useful reference for the future research on ocean wave energy conversion system.     

半潜平台浮子式系泊系统参数优化研究

通过在系泊缆中设置浮子可以改善系泊系统性能,降低平台运动响应。建立浮子式系泊系统的数值计算模型,验证浮子式系泊系统数值计算方法,详细分析浮子设计参数(设置位置和净浮力大小)变化对系泊缆张力特性与平台运动的影响规律,并根据得到的规律选择了优化的浮子系泊系统方案。最后对优化方案进行评估,表明优化方案可以显著降低系泊中的平台水平运动,尤其是低频运动,同时系泊缆张力变化不大,仍满足安全要求。研究结果可为今后浮子式系泊系统设计提供参考。     

Numerical and Experimental Analysis of A Ducted Propeller Designed by A Fully Automated Optimization Process Under Open Water Condition

A fully automated optimization process is provided for the design of ducted propellers under open water conditions, including 3D geometry modeling, meshing, optimization algorithm and CFD analysis techniques. The developed process allows the direct integration of a RANSE solver in the design stage. A practical ducted propeller design case study is carried out for validation. Numerical simulations and open water tests are fulfilled and proved that the optimum ducted propeller improves hydrodynamic performance as predicted.     

多目标规划在哈尔滨市地下水资源管理中的应用

建立了哈尔滨市地下水资源优化管理模型 ,将求解多目标最优化问题的约束法和线性加权法相结合 ,给出了一种综合解法 ,并将此法应用于所建立的水资源优化管理模型的求解。其结果可为该地区地下水资源的管理提供有参考价值的科学依据。     

Geometric-constrained multi-view image matching method based on semi-global optimization

Targeting at a reliable image matching of multiple remote sensing images for the generation of digital surface models, this paper presents a geometric-constrained multi-view image matching method, based on an energy minimization framework. By employing a geometrical constraint, the cost value of the energy function was calculated from multiple images, and the cost value was aggregated in an image space using a semi-global optimization approach. A homography transform parameter calculation method is proposed for fast calculation of projection pixel on each image when calculating cost values. It is based on the known interior orientation parameters, exterior orientation parameters, and a given elevation value. For an efficient and reliable processing of multiple remote sensing images, the proposed matching method was performed via a coarse-to-fine strategy through image pyramid. Three sets of airborne remote sensing images were used to evaluate the performance of the proposed method. Results reveal that the multi-view image matching can improve matching reliability. Moreover, the experimental results show that the proposed method performs better than traditional methods.     

一类全局最优化问题的动态隧道方法

研究了连续变量函数的全局最优化问题 ,给出了动态隧道方法。该动态隧道方法由局部搜索和动态隧道 2个阶段构成。在局部搜索阶段用了动态系统方法。对全局最优化问题的实例进行了数值实验 ,数值结果表明了该方法的稳健性和有效性。