Efficient detection of pathogen virus in sand dabs, Paralichthys olivaceus using loop-mediated isothermal amplification (LAMP)

Viral hemorrhagic septicemia virus (VHSV) and marine birnavirus (MABV) are the causative pathogens for some of the most explosive epidemics of emerging viral diseases in many Asian countries, leading to huge economic losses in aquaculture. Rapid molecular detection for surveillance or diagnosis has been a critical component in reducing the prevalence of pathogen infection. The loop-mediated isothermal amplification (LAMP) of DNA is currently one of the most commonly used molecular diagnostic tools, as it is simple, quick, and easy to amplify target DNA under isothermal conditions. In the present study, a novel and highly specific LAMP assay for the sensitive and rapid detection of VHSV and MABV infection in fish was developed. Using a set of synthesized primers matching a specific region of the genome, the efficiency and specificity of the LAMP assay were optimized in terms of the reaction temperature and DNA polymerase concentration, as they are the main determinants of the sensitivity and specificity of the LAMP assay. In particular, we demonstrated that our assay could be applied to efficient detection of VHSV and MABV infection in the wild fish, Paralichthys olivaceus. Our results demonstrate the simplicity and convenience of this method for the detection of viral infection in aquatic organisms.     

磁力仪探头之间安全距离的实验与认识

为了加强对磁法勘察工作的管理和保证工作质量,为适应科学技术的进步和发展,特别是磁法仪器的不断提升和更新,适时修订相关的技术规程或者工作规范是十分必要的.修订技术规程或者工作规范亦应该契合实际,一是规定明确,二是在保证质量的前提下便于生产单位参照执行.笔者就《地面高精度磁测技术规程》的关于仪器噪声检测时探头的安全间距规定进行探讨,结合相关的磁测技术规定和教科书的要求及专门的试验结果,通过专门实验进行分析讨论,引用实例进行说明,认为并建议对上述规定进行修订.     

二元混合气体等温吸附实验结果与扩展Langmuir方程预测值的比较

通过实验获得了CH4和N2及其二元混合气体的等温吸附方程，并利用扩展Langmuir方程对二元混合气体的吸附行为进行了预测。结果表明，利用纯CH4气体的等温吸附方程和扩展Langmuir方程对二元混合气体吸附行为的预测结果，均与二元混合气体的等温吸附实验数据存在较大误差。所以，当煤层气中合有一定数量的非甲烷气体时，只有通过混合气体的等温吸附实验，才能获得准确的吸附特性参数。     

Imaging distribution patterns of magnetic minerals by a novel high-Tc-SQUID-based field distribution measuring system: application to Permian sediments

A newly developed field distribution measuring system based on a high- T _c SQUID has been employed in the study of magnetic mineral distribution in several Permian sedimentary rocks. The instrument consists of a small, 1.4×1.4 mm sized YBaCu-oxide SQUID magnetic field sensor that is operated in a thin-walled dewar, so that the sample's surface, at room temperature, can be scanned at a distance of only ∼1.5 mm. The samples were subjected to a saturation remanence perpendicular to the surface and the scanning measurements in zero field reveal that the magnetization might be carried by only a small part of a sample, in one case associated with secondary oxide phases. High-resolution magnetic scans can aid in the interpretation of the magnetic remanence acquisition process.     

中国区域地磁异常的提取及分析

区域地磁异常的提取对于研究地壳区域的、深部的情况是很有益的。为此,采用地磁台站的多分量磁场强度,经过处理获得了区域地磁异常,提供了丰富的地质信息。     

氢质子弛豫过程

论述了原子核系统磁化强度的的弛豫原理,分析了含水地层中氢质子磁化强度的弛豫过程,推导出磁化强度的布洛赫方程,从而给出接受线圈中感应电动势的衰减规律,通过计算得到磁化强度各分量随时间的衰减过程以及感应电动势的衰减曲线,分析了纵向弛豫和横向弛豫时间常数与磁化强度各分量和感应电动势的关系。     

3种人工湿地基质吸附污水中氨氮的性能与基质筛选研究

通过等温热力学吸附实验,比较人工湿地的基质高钙废渣、改性赤泥和火山石对污水中氨氮的去除效果。结果表明,3种基质的氨氮吸附过程都可以用Langmuir吸附等温方程进行拟合;高钙废渣、改性赤泥和火山石对氨氮的最大吸附量分别为26.27mg/g、6.15mg/g和0.98mg/g;经高钙废渣处理的污水,出水最清澈;高钙废渣对氨氮的吸附效果最好。用吸附效果最好的高钙废渣进行了模拟人工湿地吸附氨氮的基质柱实验,以高钙废渣为基质的模拟人工湿地对生活污水中氨氮的去除率为20%～42%。