Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.     

地缘经济关系测度与分析的理论方法探讨--以云南省为例

文章首先简要分析了地缘经济学的发展进程和研究内容，提出以保护国家利益增强综合国力为主要研究目的的地缘经济理论在一定程度上可以指导不同级别区域经济合作策略的制定。然后在探究了地缘经济关系涵义的前提下，设计了地缘经济关系的测度体系，并用该方法对云南省地缘经济关系进行了数量测度和类型判别，进而对该省地缘经济关系进行了分析和评价，对经济发展提出了建议。     

2001-2010 年安徽省县域经济空间演化

选择安徽省2001-2010 年县域人均GDP为研究指标,综合运用G指数、经济重心、标准差椭圆以及灰色G(1, 1)模型对县域经济的空间演化进行分析预测,最后结合位序规模原理,对空间差异的机理进行分析.总体上安徽县域经济表现出微弱的空间集聚分布态势,呈现出“南北低中间高”、“西低东高”的空间分布格局.县域经济重心在117.57°~117.6°E,31.67°~31.76°N之间变动,有向东南方向移动的趋势.标准差椭圆总体上变化幅度不大,基本上以省会经济圈为核心,范围覆盖了皖江城市带大部分地区,县域经济的空间分布呈现出西北—东南格局,并且这种格局有向正北—正南方向转变的趋势.安徽县域经济满足位序规模原理,其发展状况呈低水平分散均衡型.资源禀赋与交通区位、中心城市发展状况、区域政策是导致安徽省县域经济空间差异的主要原因.     

全国分县土地经济系数计算方法及比较

阐述土地经济系数的设置意义和目标,针对全国分县土地经济系数计算,提出并分析比较了统计指标法和分区样点法等思路及其数学处理方法。其中统计指标法中的农业产值消耗法基础数据来源可靠、便利,便于操作,计算结果较好,符合土地经济系数含义要求。     

基于主成分神经网络的台风灾害经济损失评估

本研究建立了浙江省台风灾害直接经济损失评估模型。把浙江省台风灾害直接经济损失资料换算成直接经济损失指数,运用主成分分析法对表示致灾因子、孕灾环境与承灾体的评估因子进行数据处理,提取主成分作为BP神经网络模型的输入,从而建立评估模型。模型历史拟合结果和实际一致。在2007年和2008年影响浙江省的5个台风的实际评估中,强台风"Vipa"灾后评估值比实际值偏大2.16,其余4个台风灾后评估值比实况偏大0.2~0.7,反映了人们对影响大的台风防灾减灾工作的重视和防灾减灾效果。根据台风开始影响时过程风雨预报值进行预评估,过程风雨预报值较准确的台风,预评估结果和灾后评估值一致;过程风雨预报值误差较大的台风,预评估效果较差。因此,该模型可用于实际台风灾害直接经济损失评估,提高台风影响前风雨预报准确率是提高预评估准确率的关键。     

Minor planet short periodic perturbations: The indirect part of the disturbing function

Leverrier's development of the indirect part of the disturbing function has been extended to include terms up to degree 4 in eccentricity and inclination; the resulting series has been expressed with respect to a fixed plane, and in a computer readable form (a list of integers). Tests have been performed for the relative significance of the terms of degrees 2, 3 and 4, and estimates have been obtained for the accuracy of the short periodic perturbations of a minor planet, and of the corresponding mean orbital elements. It was found that: (i) even in extreme cases, the indirect part of the disturbing function gives rise to very small short periodic perturbations; (ii) bodies of very high eccentricity/inclination and those close to mean motion resonances are most significantly affected; (iii) indirect perturbations for minor planets can be computed up to the degree 2 terms only, without any significant loss of accuracy; and (iv) higher degree indirect perturbations appear to be important only for their contribution to the long periodic effects of higher order (with respect to the perturbing mass).     

The Mount Parnitha (Athens) Earthquake of September 7, 1999: A Disaster Management Perspective

Natural Hazards - Strong earthquakes in the proximity of densely inhabited urban areas pose one ofthe most complicated disaster management situations faced by societies today. Herethe experience...     

中国区域经济差异变化的空间特征及其政策含义研究

采用了种省区人均国内生产总值与全国平均值的离差和比离两个指标，分别判断１９９０￣１９９５年我国区域经济绝对差异和相对差异变化的空间状态，并进一步阐明了其调控区域经济差异的政策含义。     

东南亚金融危机对上海经济发展的影响及其对策初探

对中国大陆经济中心之一的上海市来说,东南亚金融危机一方面造成了区域经济发展速度的降低、企业经营困难加大、产业空间结构调整计划延期,但另一方面却同时也为上海市宏观经济特别是工业经济政策调整提供了千载难逢的大好机会。从上海市经济可持续发展的要求出发,上海市应该重新认识工业在国民经济中的主导作用,重视与国内其它地区的经济合作,重视新技术研究开发,重视工业空间布局的调整和优化