Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria

Trimethylamine N-oxide(TMAO) is widely dispersed in marine environments and plays an important role in the biogeochemical cycle of nitrogen. Diverse marine bacteria utilize TMAO as carbon and nitrogen sources or as electron acceptor in anaerobic respiration. Alteration of respiratory component according to the pressure is a common trait of deep-sea bacteria. Deep-sea bacteria from dif ferent genera harbor high hydrostatic pressure(HHP) inducible TMAO reductases that are assumed to be constitutively expressed in the deep-sea piezosphere and facilitating quick reaction to TMAO released from ?sh which is a potential nutrient for bacterial growth. However, whether deep-sea bacteria universally employ this strategy remains unknown. In this study, 237 bacterial strains affliated to 23 genera of Proteobacteria,Bacteroidetes, Firmicutes and Actinobacteria were isolated from seawater, sediment or amphipods collected at dif ferent depths. The pressure tolerance and the utilization of TMAO were examined in 74 strains. The results demonstrated no apparent correlation between the depth where the bacteria inhabit and their pressure tolerance, regarding to our samples. Several deep-sea strains from the genera of Alteromonas, Halomonas,Marinobacter, Photobacterium, and Vibrio showed capacity of TMAO utilization, but none of the isolated Acinebacter, Bacillus, Brevundimonas, Muricauda, Novosphingobium, Rheinheimera, Sphingobium and Stenotrophomonas did, indicating the utilization of TMAO is a species-speci?c feature. Furthermore, we noticed that the ability of TMAO utilization varied among strains of the same species. TMAO has greater impact on the growth of deep-sea isolates of Vibrio neocaledonicus than shallow-water isolates. Taken together, the results describe for the ?rst time the TMAO utilization in deep-sea bacterial strains, and expand our understanding of the physiological characteristic of marine bacteria.     

旋翼无人机测算风速风向技术研究

目前大部分民用无人机没有准确测量风速、风向和预警风场的功能,然而森林火灾扑救等多种场景需要无人机可以提供准确的风速。本文提出了一种基于旋翼无人机坐标数据测算风速风向的技术,通过无人机主机RTK坐标信息及方位角、倾角数据精准获取螺旋桨点相对坐标信息,选择不同负载条件下无人机以1~16 m/s的不同速度分别飞行30 s以上,根据螺旋桨坐标信息变化数值,结合风洞测试数据及风场动力学原理,研究风速与旋翼无人机倾角关系,并通过风洞试验检验该方法精度;并可根据无人机RTK推算出的螺旋桨坐标变化信息判断风向。结果表明,无人机风速与旋翼无人机倾角呈正相关关系;无人机负载加重时,对应受风速干扰的倾角会相对减小;无人机飞行受阵风干扰出现噪点的概率,高海拔区域大于低海拔区域;并建立六旋翼无人机飞行倾角的风速估算模型y=－1.043 5+1.150 1x,该模型测算风速的中误差值为0.966,绝对值小于1,满足应急指挥现场对无人机测量风速精度要求。该方法得到风速测算精度高,可以为旋翼无人机实时获取风速风向提供一种可行方法,具有一定的实用价值。     

Drainage conditions around monopiles in sand

Large diameter monopiles are typical foundation solutions for offshore wind turbines. In design of the monopile foundations in sand, it is necessary to understand the drainage conditions of the foundation soil under the design loading conditions as the soil performance (strength and stiffness) is highly dependent on the drainage conditions. This paper presents a numerical investigation into this issue, with a purpose to develop a simple design criterion for assessing the soil drainage conditions around a monopile in sand. It is found that for typical monopile foundations in sand, the drainage condition during a single load cycle is generally expected to be undrained. However, the current state-of-practice uses p-y springs derived for drained soil responses for monopile design. The impact of this discrepancy on monopile foundation design was evaluated and found to be insignificant due to the relatively low level of loading as compared to the capacity of the soil.     

高分辨率遥感影像配准与变化检测一体化处理

为了克服高分辨率遥感影像配准与变化检测作为单独环节处理的局限,该文提出了一种基于变分理论的配准与变化检测一体化处理方法。该方法将配准误差作为一种光谱变化决策因子,变化信息以权值的方式迭代反馈给变分配准模型的解算过程。为了更准确地检测建筑物这个特定目标的真实变化,该文采用多尺度最大形态学轮廓建筑物检测指数的差异作为另外一个决策因子。最后将配准误差反映的变化和建筑物检测指数的差异这两个决策因子在D-S证据理论框架下建立概率模型进行融合处理,进而得到建筑物的变化检测结果。该文选取WorldView-2数据进行实验,实验结果表明,一体化处理思路可以有效地解决单独处理的局限,从根本上解决配准误差对变化检测结果的影响以及由于变化而使配准精度降低的问题,进而提高配准和变化检测的质量。     

我国南方西南和中东部区域两次持续性低温雨雪过程与关键环流系统的关系研究

利用NCEP再分析资料和国家气象信息中心提供的753站逐日气温和降水资料,对比分析了我国南方西南和中东部区域两次持续10 d以上的低温雨雪过程,结果表明:(1)两次过程中欧亚大陆中高纬东亚大槽均加深,但环流形势有差异。西南过程呈现"北高南低"形势,关键脊区在贝加尔湖,而中东部区域过程"北高南低"和"西高东低"形势共存,关键脊区从乌拉尔山延伸至贝加尔湖。两次过程异常的环流与北大西洋向东传播的波列有关。(2)西南过程关键脊区提前过程3 d发展并东移至贝加尔湖,形成稳定形势;而中东部区域过程关键脊区提前过程一周发展,在开始日达最强。两次过程均伴随蒙古高压东移南压使地面降温,500 hPa关键脊区超前蒙古高压2 d变化。西南过程降温主要受到冷平流和绝热冷却影响,而中东部区域过程主要受到冷平流的影响。(3)西南过程水汽来自孟加拉湾,只受南支槽支配。中东部区域过程水汽来自孟加拉湾、南海和西太平洋,由南支槽和西太平副热带高压的共同影响。两次过程水汽正收支主要来自南边界。     

三杯风速传感器非水平风场测量性能

为解决三杯风速传感器在计量检定条件下与观测场景中环境差异所导致的测量数据误差,致力于研究空气流速计量标准在量值传递过程中的真实性、准确性和一致性,为新一代三杯风速传感器作为计量器具的新产品型式评价提供思路和参考指标,依据杯式测风仪测量方法与自动气象站风速风向传感器检定规程,并在实验中加入了主体由角度编码器构成的自动化转盘系统,设计了三杯风速传感器在非水平风场内测量性能水平实验。通过调整三杯风速传感器在风洞试验段内的倾斜角度,模拟其在自然界非水平风场中的测量状态,同步采集风洞的标准指示风速、三杯风速传感器的实测风速以及其相应的倾斜角度,计算示值误差,利用方差分析、趋势分析、相关性分析和线性回归分析等统计方法,对不同倾斜角度下三杯风速传感器示值误差进行研究,得出了三杯风速传感器在风洞试验段内的示值误差与实测风速和倾斜角度之间的相关关系,提出了三杯风速传感器在非水平风场下的测量性能指标。研究了三杯风速传感器在非水平风场中实测风速与标准风速和倾斜角度的回归关系,提出了三杯风速传感器在计量环境下非水平风场中数据的量值传递修正算法。     

贵州省不同强度区域性凝冻过程环流形势对比分析

利用贵州省84个气象站逐日观测资料以及再分析资料,对4种不同强度区域性凝冻过程进行对比分析。结果表明：500 hPa位势高度场上中高纬度的亚洲东部区域距平场呈现“+-”的分布或有切断低压分布,贝加尔湖至中国华北地区以经向环流为主;850 hPa风场上云南南部以南受偏南风和西南风控制,并且在江南至华南存在西南或西风急流,是4种不同强度凝冻过程中形势场共性特征。500 hPa高度场上中高纬度地区呈两槽一脊或一槽一脊分布;风场上850 hPa东北风回流和700 hPa西南急流形成上暖下冷的形势场,同时850 hPa形成稳定低层切变线;温度场上存在冷-暖-冷的夹心结构,近地面层0 ℃线维持在900 hPa以下,均是较强等级以上的区域性凝冻过程中形势场共性特征。而对于一般性区域性凝冻过程,500 hPa位势高度场上呈多槽脊分布,风场上是否存在东北风回流和低层切变线,温度场上是否存在冷-暖-冷的夹心结构以及近地面层0 ℃线位置等特征均不统一。温度剖面图上,当近地面层0 ℃线位置最低时或出现冷-暖-冷的夹心结构时段与凝冻过程影响范围最广、灾情最重的时间段对应。     

涌浪对风浪能量影响的实验研究

在实验室风浪水槽中进行纯风浪和混合浪波面位移观测,研究波长较长的规则波对风浪能量的影响.本文用混合浪和纯风浪中的风浪显著波的零阶谱矩之比代表混合浪中的风浪与纯风浪能量之比,并以此表征涌浪对风浪能量的影响.研究了该能量比随涌浪波陡S、风区x、波龄倒数u/C、涌浪频率与纯风浪谱峰频率之比fs/fwp的变化规律.结果表明,涌浪对风浪能量的抑制作用随涌浪波陡的增加、波龄倒数的增大及涌浪频率与纯风浪谱峰频率之比的增大而增强.发现该能量比依赖于无因次量R=(1+80(πS)2)1.9(fs/fwp)0.9(u/C)0.27,并拟合得到2者的经验关系.此外,本文实验还发现,在某些情况下,涌浪的存在使风浪能量增加.     

高精度高性价比的GPS定向仪的设计

介绍了一种高精度、高性价比的GPS定位定向仪的设计及实现（〈1mil,2σ）方法,系统利用了实际应用中的有利约束条件,对基于LAMBDA方法的载波相位整周模糊度解算算法进行了改进,经过系统工程设计,在实际应用中实现了单频、高精度、快速定向数据的输出。可以满足绝大多数环境下的使用需求,大大降低了系统成本。     

A real-time, event-triggered storm surge forecasting system for the state of North Carolina

A new real-time, event-triggered storm surge prediction system has been developed for the State of North Carolina to assist emergency managers, policy-makers and other government officials with evacuation planning, decision-making and resource deployment during tropical storm landfall and flood inundation events. The North Carolina Forecast System (NCFS) was designed and built to provide a rapid response assessment of hurricane threat, accomplished by driving a high-resolution, two-dimensional, depth-integrated version of the ADCIRC (Advanced Circulation) coastal ocean model with winds from a synthetic asymmetric gradient wind vortex. These parametric winds, calculated at exact finite-element mesh node locations and directly coupled to the ocean model at every time step, are generated from National Hurricane Center (NHC) forecast advisories the moment they are inserted into the real-time weather data stream, maximizing the number of hours of forecast utility. Tidal harmonic constituents are prescribed at the open water boundaries and applied as tidal potentials in the interior of the ocean model domain. A directional surface roughness parameterization that modulates the wind speed at a given location based on the types of land cover encountered upwind, a forest canopy sheltering effect, and a spatially varying distribution of Manning’s–n friction coefficient used for computing the bottom/channel bed friction are also included in the storm surge model. Comparisons of the simulated wind speeds and phases against their real meteorological counterparts, of model elevations against actual sea surface elevations measured by NOAA tide gauges along the NC coast, and of simulated depth-averaged current velocities against Acoustic Doppler Current Profiler (ADCP) data, indicate that this new system produces remarkably realistic predictions of winds and storm surge.