南极冰藻 Chlamydomonas sp.ICE-L的 cDNA文库构建及品质检测

为构建南极冰藻Chlamydomonassp.ICE-L的cDNA文库,提取对数生长期南极冰藻ICE-L的总RNA,以此为模板,通过PowerScript逆转录酶逆转录合成第一链cDNA;再以第一链cDNA产物为模板,用LD-PCR合成第二链cDNA。该cDNA产物经分级分离转入大肠杆菌中,即获得南极冰藻ICE-L的cDNA原始文库,其滴度为1.6×106cfu/mL。扩增后的cDNA文库的滴度为1.0×1010cfu/mL。用PCR方法测得文库的重组率大于97%,插入cDNA的长度为0.5~1.8 kb,0.9 kb以上插入片段占50%以上。取适量扩增文库稀释并铺平板,挑取72个独立菌落,对其中22个独立菌落所插入的cDNA进行测序,克隆到了一个具有5′和3′非编码区的40S ribosomalprotein S5全长基因序列,GenBank收录号为AM167929。     

2004～2005年度冬季渤海海冰数值预报

本文简要介绍了2004-2005年度冬季渤海的天气与冰情,总结了该年度海冰数值预报情况,对Nudging同化方法在海冰数值预报中的应用结果进行了对比分析。     

南极长城站附近海区冰藻色素的初步分析

本文报道了 1 988年 6 - 1 2月 (结冰期 )南极长城站附近海区冰藻色素的分离、鉴定结果。探讨了该海区冰藻色素的种类组成 ,季节变化以及冰藻色素在海洋生态系中的作用。结果表明 ,该海区冰藻已分离出色素 1 5种 ,可鉴定的有 1 3种 ,分别为 :胡萝卜素 ,脱镁叶绿素 a,叶绿素 a、b、c,叶黄素 ,岩藻黄质 ,脱植基叶绿酸 a,紫黄质 ,脱镁叶绿素 c,叶绿酸 a,叶绿素 c的衍生物 ,di-adinoxanthin,二种色素未能鉴定。该海域冰藻色素具明显的季节变化。     

15万年以来极地冰芯、黄土、深海沉积的对比研究与全球变化

由极地冰芯记录、中纬黄土记录和深海沉积记录的对比可得，１５万年以来全球变化的基本框架为：末次间冰期，从距今１４万年开始到约１２．５万年达鼎盛，可分为五个阶段，即冰芯中三次高温期夹两次低温期；黄土中三层古土壤夹两层黄土；深海沉积中三次高海面夹两次低海面。末次冰期，距今８万年到１万年，可分为三个阶段，早、晚冰段和中间的间冰段。早、晚冰段盛期气候寒冷程度相当，但晚冰段最低温持续期更长，造成海面比早期更低。中间的间冰段，至少可分为７个亚段，即４次暖期（４次弱成土古土壤）夹３次冷期。全新世与末次间冰期对比，总体气候变化较平缓。在上述基本框架中，近几年发现，在末次冰期存在许多短周期、高频快速变化事件。这些事件，温度变化幅度达７℃，变化速率为几年到几十年，对应的环境变化速率可能更快。这些事件的引发机理目前还不清楚，有待进一步研究。     

CLIMATE-CONTROLLED GLACIAL EROSION IN THE UNCONSOLIDATED SEDIMENTS OF NORTHWESTERN EUROPE,BASED ON A GENETIC MODEL FOR TUNNEL VALLEY FORMATION

The development of large erosive subglacial forms in unconsolidated sediments is generally attributed to the eroding power of subglacial meltwater flowing under high pressure conditions. Most explanations, however, differ in the source of meltwater and the speed at which it erodes the subglacial bed. Based on the geometry of deep tunnel valleys and glacial basins in northwestern Europe, a reconstruction of subglacial hydrological conditions during the development of subglacial depressions is made. It is demonstrated that the flow of subglacial meltwater in subglacial channels under high glaciostatic pressures is only capable of eroding large volumes of sediment as long as there is imminent glaciohydrological instability. For the thick aquifers in northwestern Europe, this instability is achieved when large quantities of supraglacial meltwater are available. Furthermore, a theoretical definition is given for maximum depression depth to be reached by subglacial erosion. It is shown that this maximum depth is strongly related to average air temperatures during deglaciation and that glacier bed lowering is to be expected during any final phase of glaciations. The theoretical framework presented enables a tentative comparison between large-scale glacial morphology of different glaciations in northwestern Europe.     

Interdecadal oscillations of temperatures in 1903 - 2002 over the Antarctic Peninula

In the paper, by use of the monthly mean temperature data of 12 stations in the vicinity of Antarctic Peninsula, the temperature series during 1903 - 2000 is founded and the interdecadal oscillation of the temperature are discussed. The results indicate that 1） There are three jumps during 1919 - 1923, 1947 - 1953 and 1976 - 1982 in recent hundred years and the stable climate step between two jump points lasted about 30 years. 2） Annual mean temperature is increased by 0. 730℃ in an echelon during 1903 -2000, the warming extent is dissimilarity in each season, the maximum of warming is in the winter and the minimum of warming is in summer. 3） The ice decline trend is presented in the index of Ice concentration in the vicinity sea of Antarctic Peninsula, which shows a -0. 2053/10a drop, and the decrease trend of the ice concentration index in summer half year （Dee-May） is found much more obviously than that in winter half year （Jun-Nov）. 4） There is better negative relationship between the temperature and the Ice concentration index in Antarctic Peninsula and its vicinity sea, which correlation coefficient of is exceed the significance level of 5% in summer, autumn and annual.     

20 years of mass balances on the Piloto glacier, Las Cuevas river basin, Mendoza, Argentina

Climatic changes of the 20th century have altered the water cycle in the Andean basins of central Argentina. The most visible change is seen in the mountain glaciers, with loss of part of their mass due to decreasing thickness and a substantial recession in the last 100 years. This paper briefly describes the results of glacier mass balance research since 1979 in the Piloto Glacier at the Cajón del Rubio, in the headwaters of Las Cuevas River, presenting new results for the period 1997–2003. Very large interannual variability of net annual specific balance is evident, due largely to variations in winter snow accumulation, with a maximum net annual value of + 151 cm w.e. and a minimum value of - 230 cm w.e. Wet El Niño years are normally associated with positive net annual balances, while dry La Niña years generally result in negative balances. Within the 24-year period, 67% of the years show negative net annual specific balances, with a cumulative mass balance loss of - 10.50 m water equivalent (w.e.). Except for exceptions normally related to El Niño events, a general decreasing trend of winter snow accumulation is evident in the record, particularly after 1992, which has a strong effect in the overall negative mass balance values. The glacier contribution to Las Cuevas River runoff is analysed based on the Punta de Vacas River gauge station for a hypothetical year without snow precipitation (YWSP), when the snowmelt component is zero. Extremely dry years similar to a YWSP have occurred in 1968–1969, 1969–1970 and 1996–1997. The Punta de Vacas gauge station is located 62 km downstream from Piloto Glacier, and the basin contains 3.0% of uncovered glacier ice and 3.7% of debris-covered ice. The total glacier contribution to Las Cuevas River discharge is calculated as 82 ± 8% during extremely dry years. If glacier wastage continues at the present trend as observed during the last 2 decades, it will severely affect the water resources in the arid central Andes of Argentina.     

The effect of refreezing on the isotopic composition of melting snowpack

The isotopic composition of solid and liquid portions of natural melting snowpack is investigated in detail by the separating of liquid water from snow grains at different depths of the snowpack. The slope of the δD–δ¹⁸O line for the liquid phase is found to be lower than for the solid phase. This is proved to be due to the isotopic fractionation occurring in the melt–freeze mass exchange within the snowpack. Melting of the snowpack has no clear impact on the δD–δ¹⁸O line for the solid phase, but the slope of the δD–δ¹⁸O line for the liquid shows an overall slight decrease in the melting period. When the snowpack is refrozen, the refreezing process would inevitably cause the slope of the solid phase to decrease because of the discrepancy between the slopes of the two phases. Thus the slope of the solid would become lower and lower as the diurnal melt–freeze episodes cycle throughout the melting season. This effect is then demonstrated by looking into the isotopic composition changes of glacier firn. The extent of the effect depends on the snowpack properties and environmental conditions. The slope changes also result in a decreasing trend in deuterium excess. Copyright © 2007 John Wiley & Sons, Ltd.