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21.
The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological
specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine
bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed
by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent
microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration
and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were
flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based
on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is
easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement
of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as
a new tool to study the fine structures of marine bacteria.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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用焦锑酸钙沉淀的电镜细胞化学方法,研究了白骨壤叶片衰老过程中叶肉细胞Ca^2 水平的变化.结果表明,在白骨壤幼叶和成熟叶叶肉细胞中,焦锑酸钙沉淀颗粒大量出现在液池和细胞间隙中,细胞壁中也可见少量沉淀,而细胞基质中则看不到焦锑酸钙沉淀.在衰老叶中,细胞基质和细胞膜上焦锑酸钙沉淀增加,而液泡和细胞间隙中的锑酸钙沉淀则显著减少,并且叶绿体外膜部分破损,结构破坏,核膜与液泡膜内部结构模糊,叶绿素含量、净光合速率、气扎手率和蒸腾速率显著下降.Ca^2 的区域性分布的变化与植物叶片衰老密切相关。 相似文献
25.
采用透射电镜技术,研究了3种鲈形目鱼类褐菖鲉Sebastiscus marmoratus、黑鲷Sparus macrocephalus及平鲷Rhabdosargus sarba(Forskal)精子发生早期精原细胞、初级精母细胞及次级精母细胞的超显微结构变化。结果表明,3种鱼生精细胞形态结构及代谢活动变化主要体现在核的变化、线粒体数目与结构的变化及高尔基体和溶酶体的行为变化。3种鱼核的变化基本一致:细胞核由近圆形或椭圆形转变为多角形;核质逐渐浓缩;核仁从明显结构至完全消失。褐菖鲉精原细胞时期线粒体分布较多,并出现拟染色质与线粒体相粘附;而平鲷与黑鲷精原细胞时期的线粒体分布较少,无拟染色质。3种鱼高尔基体与溶酶体的变化也有相似性。黑鲷的初级精母细胞中还观察到典型的环层状的髓样小体的结构。 相似文献
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根据产业经济学的观点,提出我国地理信息产业目前处于产业扩张阶段的观点,推动产业规模迅速扩大成为行业发展的主要目标。由于派生性特点,寻求地理信息的本源性需求是产业完成扩张的关键问题,利用地理信息反映的价格信息支持社会公众经济博弈活动将是地理信息巨大的本源性需求。本文建议在产业扩张阶段应当瞄准社会公众、探索对公众生活进行博弈支持的服务方式。 相似文献
28.
加强地理信息资源建设和开发利用的思考 总被引:1,自引:0,他引:1
地理信息资源的建设和开发利用在准确掌握国情国力、提高管理决策水平、管理社会公共事务、处理经济社会发展等重大问题中将发挥越来越重要的作用。本文在对发改委、测绘、国土、环境保护等部门进行调研的基础上,从我国地理信息的获取和利用现状、地理信息资源建设和开发利用政策法规的制定、产业发展以及国家安全等四个方面进行了研究,并提出了建议。 相似文献
29.
Analysis of fly-ash particles in lake sediments has become increasingly important in studies of environmental pollution and lake acidification history. Most fly-ash studies have concerned black spheroidal carbonaceous particles (SCP)(>5 m) produced from oil and coal combustion. This review paper provides a summary of this technique and its application, and focusses on our investigations in Sweden between 1979 and 1993. It consists of five parts: i) preparation and analysis methods, ii) historical trends in atmospheric deposition, iii) geographical surveys of atmospheric deposition, iv) sediment dating, and v) studies of sedimentation processes in lakes. Methods for preparation and analyses of SCP have been developed and applied to investigations using sediment, soil and snow samples. Stratigraphic trends of SCP concentrations in lake-sediment cores reflect the consumption history of fossil fuels. A characteristic temporal SCP pattern, with a marked concentration increase beginning after the 1940's and a peak in the early 1970's, has been recognized in most Swedish lakes and elsewhere in Europe. A survey of SCP concentrations in surface sediments of >100 lakes covering Sweden demonstrated that polluted areas in southern Sweden had >100 times higher SCP concentrations than clean areas in the north. The spatial distribution of SCP over Sweden is similar to the deposition pattern of long-range transported airborne pollutants, such as excess sulphate monitored by network stations. SCP also accumulate in soils, and soil analyses can be used for determining the integrated historical deposition of SCP at the local or regional scale. Finally, SCP have been used for indirect dating of sediment cores and as a marker to assess sediment distribution patterns within lake basins. 相似文献
30.
James L. Coleman Jr. 《Natural Resources Research》1995,4(3):273-288
The American whaling industry rose from humble beginnings off Long island to become an international giant. In its peak year, 1846, 735 ships and 70,000 people served the industry. As whale stocks and reserves decreased, whalers were forced to go farther and farther from their New England home ports. Voyages became longer, and risks on required return-on-investment became higher. The easy money of Atlantic and Pacific whaling was no more: the only remaining profitable ventures were to Arctic and Antarctic waters. Many ships returned empty, if at all. in 1871, most of the Arctic whaling fleet was crushed by early winter ice and lost. This calamity, in conjunction with the long-term diminishing whale stocks, the diversion of investment capital to more profitable ventures, and the discovery, development, and refinement of abundant petroleum crude oil, struck the death blow to the American whaling industry. By 1890, only 200 whaling vessels were at work, and by 1971, no American commercial whaling ship sailed the world's oceans.It is apparent that no single event caused the final, rapid decline. However, a single calamity, in an already stressed industry, that was self-insured and commercially interlinked, precipitated the end. Today's American petroleum industry, although adopting some principles of the American whaling industry, also has embraced other activities such as work process reengineering and customer alliances, which may preempt, or postpone, a similar catastrophic demise. 相似文献