Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

Sublittoral meiofauna with particular reference to nematodes in the southern Yellow Sea, China

Densities of major meiofaunal taxa were investigated at 34 sampling stations during six cruises by R/V Beidou to the southern Yellow Sea, China from 2000 to 2004, and the community structure of free-living marine nematodes was studied during one of the cruises in 2003. Meiofauna abundance ranged from 487.4 to 1655.3 individuals per 10 cm². Nematodes and harpacticoid copepods were the two most dominant groups, contributing 73.8–92.8% and 3.5–18.7%, respectively, to the total meiofauna abundance. One-way ANOVA showed no significant annual fluctuation of meiofauna and nematode abundances from 2000 to 2004 in the southern Yellow Sea. However, two-way ANOVA based on six stations sampled in 4 years (2001–2004) showed that there were significant differences among the six stations and the 4 sampling years for meiofauna, nematode and copepod abundance. Correlation analysis demonstrated that meiofauna abundance was mainly linked to chloroplastic pigments. Other environmental factors could not be ruled out, however. A total of 232 free-living marine nematode species, belonging to 149 genera, 35 families and 4 orders, were identified. The dominant species in the sampling area were the following: Dorylaimopsis rabalaisi, Microlaimus sp.1, Prochromadorella sp., Promonohystera sp., Cobbia sp.1, Daptonema sp.1, Leptolaimus sp.1, Halalaimus sp.2, Aegialoalaimus sp., Chromadorita sp., Parodontophora marina, Parasphaerolaimus paradoxus, Quadricoma sp.1, Campylaimus sp.1, Halalaimus gracilis, Paramesacanthion sp.1, Paramonohystera sp.1, and Metalinhomoeus longiseta. CLUSTER and SIMPROF analyses revealed three main types of nematode community (or station groups) in the sampling area, including I: coastal community, II: transitory community between coastal and YSCWM (Yellow Sea Cold Water Mass), and III: YSCWM community. Each community was indicated by a number of dominant nematode species. Bio-Env correlation analysis between the nematode community and environmental variables showed that water depth, sediment water content, organic matter, chlorophyll a (Chl-a) and phaeophorbide a (Pha-a) were the most important factors to determine the community structure.     

Influence of stratification and irradiance regime on summer phytoplankton composition in coastal and shelf seas of the British Isles

The effect of interaction between stratification and irradiance regimes on phytoplankton community structure was investigated in three shelf/coastal regions of the British Isles, each of which displayed ranges of vertical stability and light attenuation. Relationships between vertical stability, light penetration and community structure were indicated by the ordination of dominance (assessed using cell volume) of the main phytoplankton phyletic groups—diatoms, dinoflagellates and microflagellates—on a surface defined by a bulk stratification index () and water column depth, scaled by transparency (λh). Diatom- and dinoflagellate-dominated communities occupied distinctly different domains on the - λh surface, diatoms being favoured in well-mixed water columns with high values for λh and dinoflagellates dominating where stratification was strong and λh was low. Microflagellates were not abundant in any of the study areas and showed no clear ordination on the - λh surface. The domain of co-dominance of diatom and dinoflagellates on the - λh surface was narrow with small changes in the irradiance or stratification regime resulting in a switch to diatom or dinoflagellate dominance. It is suggested that loss of non-motile diatom communities in strongly stratified water columns might be a strong selective force in favour of dinoflagel-lates. However, in water columns with intermediate stratification and optical properties, the outcome of competition may be decided by physiological attributes of the two groups with respect to growth in low and fluctuating irradiances.     

Ecology of beach wrack in northern New England with special reference to Orchestia platensis

The northern New England beach wrack community with special reference to the cosmopolitan amphipod crustacean, Orchestia platensis, was examined at estuarine and open coastal habitats. Beach wrack was dominated by the plant genera Ascophyllum, Zostera, Spartina and Chondrus, and was most abundant during spring and late summer. Animal community numbers, biomass and frequency in fresh to moderately decomposed wrack were dominated by O. platensis throughout the year at all habitats; oligochaetes and Collembola were also important. The abundance of O. platensis showed high spatial and temporal variability, with low abundance generally associated with decreased amounts of wrack during colder months. An exception was the winter presence of the species at one estuarine habitat, in patchy aggregations within gravel-cobble refuges. The abundance of O. platensis averaged 1280 (0.04 m²)⁻¹, with a maximum of 7040 (0.04 m²)⁻¹. The life cycle of O. platensis is bivoltine, with summer-hatched young reaching maturity within 1 month. Laboratory studies indicate females with up to 4 broods (30 days)⁻¹, averaging 18 eggs brood⁻¹.Orchestia platensis is omnivorous, eating fresh plant tissue, live oligochaetes, Limulus eggs and diatom ‘fuzz’. The rate of laboratory consumption of algae and Zostera was 0.05 mg plant mg⁻¹ wet body weight day⁻¹. Presumptive predators of O. platensis are juvenile green crab, Carcinus maenus, and the earwig. Anisolabis maritima. The mobility, aggregation and aggressiveness of O. platensis assist the species in establishing and maintaining populations in the rigorous wrack habitat. The general competitive superiority of O. platensis over its congener, O. gammarella, and the co-occurrence of these species on both eastern and western Atlantic shores is discussed.     

A study on benthic community structure in west of the Taiwan Strait and near the Taiwan Shoals

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基于GIS空间统计分析的广电收视率评估研究——以北京市广电收视覆盖分析系统为例

本文将GIS空间信息统计分析手段应用于广播电视收视率的评估,以层次分析法为基础进行改进,采用抽样调查的方法确定权重,提出较为合理的基于空间信息统计方法的广电人口覆盖分布模型并加以软件实现。以北京市为研究区域,经导入多个版本人口数据实验,发现这种评估方式较好地修正了传统的仅仅按照面积评估覆盖率的评估模型,对于提高广电行业的信息化管理水平具有一定意义。     

CA-AR模型在人口空间场属性变化模拟中的应用

本文结合CA(cellular automata)和AR(auto-regression)提出模拟人口空间场属性变化的CA-AR模型,以1991～2006年成都市各区、县市的人口统计数据和成都市行政区划图为基础,将行政单元转化为单元网格,分别建立成都市主城区各单元格的CA-AR模型。结果显示,模拟结果的绝对误差和相对误差都较小,模拟精度较高,模型的显著性水平都比较高,说明CA-AR模型用于模拟人口空间场的人口数量变化是可行的。