极限波浪运动特性的非线性数值模拟

利用时域高阶边界元方法建立了模拟极限波浪运动的完全非线性数值模型,其中自由水面满足完全非线性自由水面条件.采用半混合欧拉-拉格朗日方法追踪流体瞬时水面,运用四阶Runge-Kutta方法更新下一时间步的波面和速度势,同时应用镜像格林函数消除水槽两个侧面和底面上的积分.研究中利用波浪聚焦的方法产生极限波浪,并且在水槽中开展了物理模型实验,将测点试验数据与数值结果进行了对比,两者吻合得很好.对极限波浪运动的非线性和流域内速度分布进行了研究.     

The penetration experiment to predict liquefaction resistance of reclaimed soils

The liquefaction resistance of the soils used to be estimated through the in situ tests, such as standard penetration test and cone penetration test; or by means of cyclic triaxial test in laboratory. However, both in situ tests and cyclic triaxial test are time-consuming and costly; this study introduces a quick and cost-effective method to evaluate the liquefaction resistance of soils under certain confining pressure in laboratory. A particular device modified from the conventional triaxial compression test apparatus, namely “Triaxial Cone Penetration Test”, was developed to obtain the peak values of cone resistance in soils so as to correlate the liquefaction resistance of the reclaimed soils evaluated by cyclic triaxial tests. The test result indicates a good correlation between the peak value of cone resistance and the corresponding cyclic stress ratio (CSR) at the state of initial liquefaction, in which the correlation for loose samples is better than that for dense samples. Besides, both peak values of cone resistance and corresponding CSR increase with fine content of soils reaches 10% and decreased with fine content varying between 10% and 50%. By examining the compositions of the soils with scanning electron microscopy and X-ray diffraction, it is found that the proportion and characteristics of the fines plays an important role on the liquefaction resistance of the reclaimed soils.     

条斑紫菜酵母双杂交文库的构建及PyMAPK5互作蛋白的筛选

为进一步研究条斑紫菜促分裂原活化激酶家族PyMAPK5的下游互作蛋白,理解其生物学功能,本研究通过酵母双杂交的方法进行其相互作用蛋白的筛选。提取不同温度和失水逆境胁迫下的RNA,利用Invitrogen体系构建条斑紫菜酵母双杂交cDNA文库,其库容为1.44×107CFU,重组率为91.8%。以pGBKT7-PyMAPK5为诱饵蛋白载体,利用共转化方法,从文库中筛选得到26个与PyMAPK5互作的候选蛋白。候选蛋白集中在光系统II相关蛋白、捕光蛋白、微管蛋白、ATP酶、GTP结合蛋白及假设蛋白等。微管蛋白、捕光蛋白、光系统II蛋白一对一验证结果为阳性,表明在酵母体内存在互作。本研究为阐明条斑紫菜PyMAPK5与其互作蛋白的关系及解析PyMAPK5下游作用机制奠定了基础。     

海底双层管单层连接管道结构受力分析

粘性高的海洋石油通常需要通过海底保温管道加温输送.温度变化会引起管道变形,并在管壁内产生较大的温度应力.同时,管道正常运营期间还受到管道内压、外压、管内流体粘滞力和土体摩擦力等环境荷载的作用.复杂的环境可能导致海底管道轴向应力过大发生破坏.为了提高铺管效率,提出了双层管单层连接管道这一特殊管道形式,并从理论上分析温度变化和环境荷载对该管道的影响,计算正常运行时管道不同位置处横截面内最大Von-Mises应力.最后得到了Von-Mises应力沿管道轴线分布情况,发现内管和单层连接管的应力一般比外管大,变径管和内管的焊缝处是Von-Mises应力最大的地方.     

Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol

1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on marine organisms are still unknown. In order to provide a better understanding of the ecological risks of 1-PEA in marine environments, this study determined the toxic effects of 1-PEA on two marine diatoms (Phaeodactylum tricornutum and Skeletonema costatum). The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h. The contents of chlorophyll a, chlorophyll c, glutathione (GSH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured at the end of the exposure period. 1-PEA was shown to significantly inhibit the growth of diatoms, with 96-h EC50 values of 257.14 mg/Land 126.46 mg/L in P. tricornutum and S. costatum, respectively. In P. tricornutum, the levels of SOD, CAT, GPx, GSH, and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC50 value. However, in S. costatum, the activities of SOD and CAT, and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC50 value. Taken together, these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.     

Effect of polysaccharide from Enteromorpha prolifera on maize seedlings under NaCl stress

In this study, a polysaccharide from Enteromorpha prolifera (EP) was extracted and its effect on maize seedlings under NaCl stress was investigated. Firstly, the components and structure of the EP were determined. We found that EP is a sulfated polysaccharide of high-molecular weight (Mw, 1 840 KDa) heteropolysaccharides and the main monosaccharide is rhamnose. The polysaccharide was applied to explore its effect on the growth of maize seedlings and its defense response under a salt stress. The results show that EP could promote the growth of maize seedlings under the salt stress. In addition, EP was shown able to significantly regulate membrane permeability and adjustment of osmotic substances such as soluble protein, soluble sugar, and proline, antioxidant enzymes containing superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. Therefore, EP is an effective salt-resistant substance for the growth of maize seedlings under NaCl stress.     

Current-induced scour beneath initially elevated subsea pipelines

When a subsea pipeline is laid on an uneven seabed, certain sections may have an initial elevation with respect to the far-field seabed, e_o, and thus potentially affecting the on-bottom stability of the pipeline. This paper focuses on quantifying the effects of the upstream dimensionless seabed shear stress, θ_∞, and Reynolds number, Re, on: (1) the maximum dimensionless seabed shear stress beneath the pipe, θ_max, to be compared to the critical shear stress in order to determine whether scour would occur and progress towards an equilibrium state; and, (2) the dimensionless equilibrium scour depth beneath the pipe, S_eq/D. Using a 2-D Reynolds averaged Navier-Stokes (RANS) approach along with the k-ω Shear Stress Transport (SST) turbulence model, a parametric study involving 243 computational fluid dynamics (CFD) simulations was conducted. The simulation results were used to develop a closed-form equation for the prediction of θ_max. Subsequently, experimental measurements of S_eq/D have been compiled from published literature, to develop a new closed-form equation for the prediction of S_eq/D with a high correlation to the experimental data. In summary, we present two closed-form equations for the prediction of θ_max and S_eq/D for pipelines with an initial e_o/D, which are applicable for both clear-water and live-bed conditions. The effects of θ_∞ and Re have been included, albeit Re having a small influence as compared to the other parameters.     

Effects of wave directionality on extreme response for a long end-anchored floating bridge

Reliable design codes are of great importance when constructing new civil engineering concepts such as floating bridges. Previously only a scarce number of floating bridges have been built in rough wave conditions and only limited knowledge of the extreme environmental conditions and the associated extreme response exists. To form a better design basis an increased understanding of the sensitivity in the structural response towards changes in short-crested sea parameters is needed. Furthermore, acquiring the necessary accuracy in simulated extreme response is often a computationally expensive endeavour and the number of simulations needed is often based on experience. The present study investigates the wave-induced short-term extreme response of a simplified end-anchored floating bridge concept for several wave environments with a return period of 100 years. The study includes convergence of the coefficient of variation for the extreme response for different realization lengths as well as number of realizations. The sensitivity in the structural response towards different main wave directions and spreading exponents is investigated and includes both transverse and vertical displacement response spectra and extreme Von Mises stress in the bridge girder cross-section. The extreme response is based on an accuracy of 2% in the coefficient of variation equivalent to 40 3-h realizations and a low sensitivity in the response is found for natural occurring spreading exponents and for main wave directions within 15° from beam sea.     

利用应力场预测热液区域——以TAG区为例

本文根据TAG区的钻探资料及岩心测试结果,建立了双层地质模型,在此基础上利用ANSYS应力软件并结合TAG热液区的地形数据对该区进行应力模拟。结果表明:热液喷口区域与最大水平应力低值区有较好的对应关系。其中仍处于活动状态的TAG丘状体区呈现明显的局部最大水平应力低值;而已经停止活动并且不具有典型喷口地形的MIR丘状体区域则处于最大水平应力的非封闭低值区域。据此,本文在TAG丘状体区域圈定了5个喷口可能区域,钻探结果揭示区内存在较好的矿化和蚀变现象,表明应力场预测法可能是一种有效的成矿预测方法。