Dehydration-related deformation during regional metamorphism, NW Sardinia, Italy

The relationship between deformation and dehydration has been investigated in Hercynian regionally metamorphosed rocks exposed on NW Sardinia. Two episodes of prograde mineral growth (M₁ & M₂) involving dehydration are recognized: growth of chlorite/phengite porphyroblasts at anchizone metamorphic conditions, contemporaneous with the first phase of deformation, D₁, and growth of biotite from chlorite and phengite coincident with the second phase of deformation, D₂. Deformation during both episodes of dehydration is characterized by penetrative axial planar foliations defined by well-developed phyllosilicate preferred orientations quantified by XRD textural goniometry, tight to isoclinal similar folds (interlimb angles <40°), and mineral-filled veins (hydrofractures) orientated parallel to axial planar foliations, that formed contemporaneously with the development of the penetrative foliations. No prograde mineral growth occurred during D₂ at chlorite-zone conditions. D₂ deformation in the absence of dehydration is characterized by non-penetrative crenulation cleavages, poorly developed phyllosilicate preferred orientations, relatively open (interlimb angles >40°), low-strain similar folds and minor brittle deformation. Systematic variations in macrofold interlimb angles, with respect to the timing of mineral growth, indicate that enhanced shortening (c. 80%) occurred during dehydration. Microfabrics show that the onset of dehydration is associated with the transition from a crenulation cleavage to a penetrative foliation. The presence of axial planar hydrofractures that formed coevally with dehydration and fabric development requires that supralithostatic fluid pressures and low differential stresses (<c. 20 MPa) accompanied dehydration. These features demonstrate a connection between the timing of dehydration and the style of deformation.     

A new approximation for pore pressure accumulation in marine sediment due to water waves

The residual mechanism of wave‐induced pore water pressure accumulation in marine sediments is re‐examined. An analytical approximation is derived using a linear relation for pore pressure generation in cyclic loading, and mistakes in previous solutions (Int. J. Numer. Anal. Methods Geomech. 2001; 25 :885–907; J. Offshore Mech. Arctic Eng. (ASME) 1989; 111 (1):1–11) are corrected. A numerical scheme is then employed to solve the case with a non‐linear relation for pore pressure generation. Both analytical and numerical solutions are verified with experimental data (Laboratory and field investigation of wave– sediment interaction. Joseph H. Defrees Hydraulics Laboratory, School of Civil and Environmental Engineering, Cornell University, Ithaca, NY, 1983), and provide a better prediction of pore pressure accumulation than the previous solution (J. Offshore Mech. Arctic Eng. (ASME) 1989; 111 (1):1–11). The parametric study concludes that the pore pressure accumulation and use of full non‐linear relation of pore pressure become more important under the following conditions: (1) large wave amplitude, (2) longer wave period, (3) shallow water, (4) shallow soil and (5) softer soils with a low consolidation coefficient. Copyright © 2006 John Wiley & Sons, Ltd.     

Vertical dynamic response of a rigid foundation embedded in a poroelastic soil layer

A simplified analytical method is presented for the vertical dynamic analysis of a rigid, massive, cylindrical foundation embedded in a poroelastic soil layer. The foundation is subjected to a time‐harmonic vertical loading and is perfectly bonded to the surrounding soil in the vertical direction. The soil underlying the foundation base is represented by a single‐layered poroelastic soil based on rigid bedrock while the soil at the side of the foundation is modeled as an independent poroelastic layer composed of a series of infinitesimally thin layers. The behavior of the soil is governed by Biot's poroelastodynamic theory and its governing equations are solved by the use of Hankel integral transform. The contact surface between the foundation base and the soil is smooth and fully permeable. The dynamic interaction problem is solved following standard numerical procedures. The accuracy of the present solution is verified by comparisons with the well‐known solutions obtained from other approaches for both the elastodynamic interaction problem and poroelastodynamic interaction problem. Numerical results for the vertical dynamic impedance and response factor of the foundation are presented to demonstrate the influence of nondimensional frequency of excitation, soil layer thickness, poroelastic material parameters, depth ratio and mass ratio on the dynamic response of a rigid foundation embedded in a poroelastic soil layer. Copyright © 2008 John Wiley & Sons, Ltd.     

Experimental study of extreme shear stress for shallow flow under simulated rainfall

The raindrop impact and overland flow are two major factors causing soil detachment and particle transportation. In this study, the turbulent characteristics of the shallow rain‐impacted water flow were investigated using a 2‐D fibre‐optic laser Doppler velocimetry (FLDV) and an artificial rainfall simulator. The fluctuating turbulent shear stress was computed using digital data processing techniques. The experimental data showed that the Reynolds shear stress follows a probability distribution with heavy tails. The tail probability increases with an increase of rainfall intensity or raindrop diameter, and it decreases with an increase of Reynolds number. A modified empirical equation was derived using both the raindrop diameter and rainfall intensity as independent variables to provide a better prediction of the Darcy‐Weisbach friction coefficient f under rainfall conditions. Copyright © 2009 John Wiley & Sons, Ltd.     

光谱对许氏平鲉消化代谢节律及生理应激研究

为探究不同光谱下许氏平鲉的消化代谢水平及外周激素节律,在5种光谱(红光、绿光、黄光、蓝光、白光,光周期12L∶12D)下,通过对许氏平鲉的血清、肠道、肝脏进行24 h (8:00,12:00,16:00,20:00,24:00)取样,结果显示:不同的光谱下许氏平鲉的消化代谢酶活性除胰蛋白酶(TRY)外均具有节律性,红光、黄光下α-淀粉酶(α-AMS)、脂肪酶(LPS)活性的峰值相位与白光组相比左移;胰蛋白酶活性无时间差异性;在绿光、黄光及红光下乳酸脱氢酶(LDH)活性的峰值相位与白光组相比发生左移,在五种光谱环境中,红、黄光影响消化酶活性峰值提前出现;丙酮酸激酶(PK)活性在绿、蓝及红光下峰值相位左移;己糖激酶(HK)的活性在绿、蓝、黄光下,其峰值相位与白光组相比发生左移,在五种光谱环境中,绿、蓝光影响代谢酶活性类峰值提前出现。许氏平鲉血清中褪黑素含量呈现昼低夜高分泌水平;皮质醇的含量表现为在白天降低,午夜显著升高至最高值后又持续降低。峰值相位的改变预示着生理节律的变动,以上结果表明不同光谱可以影响鱼类的生理代谢节律,在今后养殖过程中需要充分考虑光谱对养殖生物的生物学作用,进而制定更为合理的光照条件。     

Experimental research on mechanical characteristics and strength criterion of rock of triaxial unloading tests under different stress pathsEI北大核心CSCD

By carrying out triaxial unloading test under different stress paths on rock, the complete stress-strain curves, deformation characteristics and its strength criterion are studied. The test results show that the relation between lateral strain and confining pressure firstly is linear, and then is nonlinear in the total stage of unloading confining pressure. And its growth rate is about 3 to 5 times the growth rate of the axial strain. It shows obvious lateral dilatancy, and the degree of dilatancy is related to the unloading path. According to the area that is enclosed by confining pressure and volumetric strain curve, it can be seen that the greater the confining pressure is before unloading, the more the energy releases. Deformation modulus decreases gradually with the unloading confining pressure, and increases with the initial confining pressure; the relation between deformation modulus and confining pressure shows negative exponential distribution overall. At the same unloading paths, the reduction of the deformation modulus increases with the increase of initial confining pressure. The Poisson's ratio rises up continually, and the variation law with the confining pressure can be well described by nonlinear. Failure characteristics of rock are mainly shear failure. The strength characteristics of rock can be well characterized by using the Mohr strength criterion of power function. These conclusions provide reliable theoretical reference and guiding significance for the deep mining of the underground metal mine.     

脊尾白虾蜕皮抑制激素基因全长cDNA的克隆及其对环境胁迫的响应

为研究脊尾白虾蜕皮抑制激素基因(MIH)在环境适应中的作用,本文采用RACE技术从蜕皮间期脊尾白虾眼柄中克隆获得MIH-like基因全长c DNA序列,命名为Ec MIH。该基因全长1218bp,包括360bp的开放阅读框,420bp的5'端非编码区和394bp的3'端非编码区;开放阅读框编码120个氨基酸,包括41个氨基酸组成的信号肽和79个氨基酸组成的成熟肽,预测蛋白分子量为13.71k Da,理论等电点p I为8.02。同源性比较分析发现,脊尾白虾MIH-like基因与日本沼虾和罗氏沼虾同源性最高,分别为87%和86%。荧光定量PCR分析结果表明,脊尾白虾MIH-like基因在眼柄中的表达量最高,在卵巢中的表达量最少,而在肝脏、肌肉、鳃和血细胞中不表达。环境胁迫后该基因具有明显的时序性。p H6.5胁迫后24h和48h,眼柄和腹神经节中MIH-like基因的表达量较对照组均显著增加(P0.05);4mg/L氨氮浓度组胁迫后12h和24h,眼柄和腹神经节中MIH-like基因的表达量较对照组均显著增加(P0.05);盐度39胁迫后12h和24h,眼柄和腹神经节中MIH-like基因的表达量较对照组均显著增加(P0.05)。本研究结果表明脊尾白虾mih基因参与了环境胁迫后的机体应激反应。     

水杨酸对蛋白核小球藻(Chlorella pyrenoidosa) 生长及抗逆相关基因的影响

以蛋白核小球藻(Chlorella pyrenoidosa)为研究对象, 首先克隆了热激蛋白70(HSP70)和磷 酸甘油酸激酶(PGK)基因全长序列, 然后研究了0-20μg/mL 水杨酸对高温胁迫下HSP70 和PGK 基 因表达, 以及蛋白核小球藻生长、可溶性糖和蛋白含量的影响。结果获得了蛋白核小球藻HSP70 基 因的cDNA 序列2405 bp, 包括60 bp 的5'-非编码区(UTR)、1959 bp 的开放阅读框(ORF)和386 bp 的3'-UTR 和PGK 基因的cDNA 序列1664 bp, 包括35 bp 的5'-UTR, 1398 bp ORF 和231 bp 3'-UTR. 序列比较和分析表明该蛋白核小球藻HSP70 序列与其它绿藻的同源性高达83%-91%, PGK 为 70%-75%.荧光定量PCR 结果显示随着水杨酸浓度的增加, HSP70 和PGK 的表达量在12h 和24h 均有所增加, 而在10 μg/mL 水杨酸浓度下表达量最大, 12h 时分别为对照组的2.57 倍和1.56 倍, 24h 则为1.71 倍和1.79 倍。1-20μg/mL 水杨酸可促进该藻的比生长速率、可溶性糖和蛋白含量的升高。 本文结果表明一定浓度水杨酸对高温胁迫蛋白核小球藻有缓解作用, 且5 和10 μg/mL 水杨酸的效果 最显著。