Can dynamite-blasted reefs recover? A novel, low-tech approach to stimulating natural recovery in fish and coral populations

Throughout Southeast Asia, blast fishing creates persistent rubble fields with low coral cover and depauperate fish communities. We stabilized a 20-year-old rubble field in a Marine Protected Area in the Philippines, using plastic mesh and rock piles in replicated 17.5m(2) plots, thereby increasing topographic complexity, fish habitat, and recruitment substrate surface area. Multivariate analysis revealed fish community shifts within the rehabilitated area from that characteristic of rubble fields to one similar to the adjacent healthy reef within three years, as measured by changes in fish abundance and body size. Coral recruitment and percent cover increased over time, with 63.5% recruit survivorship within plots, compared with 6% on rubble. Our low-cost approach created a stable substrate favoring natural recovery processes. Both rehabilitation and the elimination of poaching were integral to success, emphasizing the synergism between the two and the need to incorporate both when considering mitigation.     

Sedimentary Features and Their Implications of Microdigital Stromatolites from the Mesoproterozoic Wumishan Formation at the Jixian Section in North China

<正>The Mesoproterozoic Wumishan Formation at the Jixian section in Tianjin is a set of more than 3000-m-thick stromatolitic carbonate succession.In this succession,several lithofacies units,that is,the subtidal stromatolitic biostrome,the thrombolitic bioherm,tidal-flat micritic dolomite and lagoon dolomitic shale,make up many meter-scale cycles of the peritidal carbonate type that have been nominated as the Wumishan cycles.Importantly,many microdigital stromatolites make up the stromatolitic biostrome unit of the Wumishan cycles in the lower part of the Wumishan Formation. These microdigital stromatolites have been grouped as a stromatolitic assemblage by paleontologists, that is,"Pseudogymnosolen mopanyuensis-Scuphus-Yangzhuang columnaris"assemblage.These microdigital stromatolites had also been interpreted as the aragonite(tufa) sea-floor precipitates by sedimentologists,and has further been thought as the special products of the transitional period from the sea-floor aragonite precipitates of the Archean to the clastic and muddy carbonates of the Neoproterozoic.Although there are some restrictions for the stratigraphic meaning of the concept of the stromatolitic assemblage,detailed studies on classification by paleontologists provide an important clue to understand the sedimentological meaning of the microdigital stromatolites.Furthermore,an important and obvious horizon for the end of the microdigital stromatolites was recorded in the Mesoproterozoic Wumishan Formation at the Jixian section,which provides useful information to understand the stromatolite decline occurred at c.1250 Ma and the evolving carbonate world of the Precambrian.     

两种混养方式对异枝江蓠(Gracilaria bailinae)生长性能、表面附生细菌群落及抗生素抗性基因的影响

为揭示"鱼-藻"和"鱼-虾-藻"混养对异枝江蓠(Gracilaria bailinae)生长性能、表面附生细菌群落和抗生素抗性基因(antibiotic resistance genes,ARGs)的影响,阐明异枝江蓠表面附生细菌群落与生长性能、ARGs之间的关系,利用16S rDNA高通量测序技术和Real-time qPCR技术分析了异枝江蓠表面附生细菌群落和ARGs的组成与差异,冗余分析(RDA)探讨细菌群落与生长性能、ARGs之间的关联。结果表明:(1)"鱼-虾-藻"混养会促进异枝江蓠的生长性能,增加表面附生细菌群落的多样性。(2)异枝江蓠表面附生细菌群落主要属于变形菌门、蓝藻门、浮霉菌门和拟杆菌门,不同混养方式中优势菌属组成不同,"鱼-虾-藻"混养优势菌属多样性较高。(3)"鱼-虾-藻"混养的异枝江蓠ARGs/MGEs的相对丰度大多高于"鱼-藻"混养。(4)RDA分析表明,生长性能主要与Ralstonia、Blastopirellula等显著相关,ARGs/MGEs主要与Nitrosomonas、Alteromonas、Pleurocapsa;CC-7319等显著相关。"鱼-虾-藻"混养能够增强异枝江蓠的生长性能,提高异枝江蓠表面附生细菌群落的多样性。但"鱼-虾-藻"混养能够增加异枝江蓠ARGs/MGEs的相对丰度,存在一定的生态风险。因此,在注重经济效益的同时也要关注可能存在的对人类健康的危害。研究结果将有助于海水养殖环境的优化,为大型海藻在海水养殖业中的应用与推广提供理论基础。     

Spatial patterns and environmental associations of deep scattering layers in the northwestern subtropical Pacific Ocean

The mesopelagic communities are important for food web and carbon pump in ocean, but the large-scale studies of them are still limited until now because of the difficulties on sampling and analyzing of mesopelagic organisms. Mesopelagic organisms, especially micronekton, can form acoustic deep scattering layers (DSLs) and DSLs are widely observed. To explore the spatial patterns of DSLs and their possible influencing factors, the DSLs during daytime (10:00–14:00) were investigated in the subtropical northwestern Pacific Ocean (13°–23.5°N, 153°–163°E) using a shipboard acoustic Doppler current profiler at 38 kHz. The study area was divided into three parts using k-means cluster analysis: the northern part (NP, 22°–24°N), the central part (CP, 17°–22°N), and the southern part (SP, 12°–17°N). The characteristics of DSLs varied widely with latitudinal gradient. Deepest core DSLs (523.5 m±17.4 m), largest nautical area scattering coefficient (NASC) (130.8 m2/n mile2±41.0 m2/n mile2), and most concentrated DSLs (mesopelagic organisms gathering level, 6.7%±0.7%) were observed in NP. The proportion of migration was also stronger in NP (39.7%) than those in other parts (18.6% in CP and 21.5% in SP) for mesopelagic organisms. The latitudinal variation of DSLs was probably caused by changes in oxygen concentration and light intensity of mesopelagic zones. A positive relationship between NASC and primary productivity was identified. A four-months lag was seemed to exist. This study provides the first basin-scale baselines information of mesopelagic communities in the northwest Pacific with acoustic approach. Further researches are suggested to gain understandings of seasonal and annual variations of DSLs in the region.     

2021年秦皇岛近岸典型海域浮游植物群落结构的时空变化特征

为了解秦皇岛近岸典型海域浮游植物的群落特征及其影响因素,基于2021年4~10月在秦皇岛金梦海湾、东山浴场两个典型海域7个航次的逐月调查结果,对该海域网采浮游植物种类组成、优势种、丰度、多样性等特征及其与环境因子的关系进行了研究,并对两个典型海域海水水质和浮游植物群落之间的差异进行了比较。结果表明:研究海域共检出浮游植物3门45属92种(包含变种和变型),其中硅藻门(Bacillariophyta)32属67种,甲藻门(Pyrrophyta) 12属24种,金藻门(Chrysophyta)1属1种,硅藻门占绝对优势。全年优势种共26种,其中硅藻20种,甲藻6种,且多出现在4~7月,未出现7个月份共有的优势种。浮游植物丰度在8月达到全年最高值,为1.01×10⁸ cells/m³,其余月份浮游植物丰度均在10⁶ cells/m³量级。聚类分析表明,研究海域浮游植物群落有明显的季节更替现象。生物与环境(BIOENV)相关分析表明,水温和盐度是影响研究海域浮游植物群落结构的主要环境因子。两个典型海域浮游植物种类组成差别不大,但金梦海湾海域的营养盐环境和水动力条件更适合甲藻生长。     

Phytoplankton diversity in a tropical bay,North Borneo,Malaysia as revealed by light microscopy and Next-Generation Sequencing

Assessments of phytoplankton diversity in Sabah waters, North Borneo, have primarily relied on morphology-based identification, which has inherent biases and can be time-consuming. Next-Generation Sequencing (NGS) technology has been shown to be capable of overcoming several limitations of morphology-based methods. Samples were collected from the Sepanggar Bay over the course of the year 2018 in different monsoon seasons. Morphology-based identification and NGS sequencing of the V8–V9 region of the 18S LSU rDNA were used to investigate the diversity of the phytoplankton community. Microscopy and NGS showed complementary results with more diatom taxa detected by microscopy whereas NGS detected smaller and rarer taxa. The harmful algal genera in the study site comprised of Skeletonema, Margalefidinium, Pyrodinium, Takayama, and Alexandrium as detected by NGS. This study showed that that an integrative approach of both morphological and molecular techniques could provide more comprehensive information about the phytoplankton community as the approach captured quantitative variability as well as the diversity of phytoplankton species.     

渤海湾浮游桡足类群落特征与影响因素

采用浅水Ⅰ型浮游生物网,分别于2017年春、夏、秋和冬季在渤海湾海域采集浮游动物,基于网采样品数据分析了桡足类群落的组成种类、生物多样性和丰度分布特征及环境因子与桡足类群落特征之间的关系。结果如下：调查海域共出现桡足类20种,其中秋季种类最多达到13种,春季和冬季分别为12种和9种,夏季最少为8种。渤海湾海域桡足类丰度的平均值在春季最高,达到了882.5 ind/m3。夏季和秋季分别为84.0 ind/m3和48.4 ind/m3。冬季最低,为11.8 ind/m3。桡足类在秋季时,其香农-威纳指数和物种丰富度指数的平均值达到了最高,夏季时桡足类的香农-威纳指数平均值达到了最低,桡足类在春季时,其物种丰富度指数的平均值达到了最低。通过分析环境因子与桡足类丰度间的相关性,结果显示：温度、盐度、溶解氧、磷酸盐、硅酸盐、pH和浊度等环境因子会对渤海湾海域桡足类群落结构的季节变化产生显著影响。春季桡足类丰度的总体分布趋势与叶绿素水平分布的趋势相似;夏季和冬季桡足类丰度的总体分布趋势与温度水平分布的趋势相似;秋季桡足类丰度的总体分布趋势与温度水平分布的趋势相反。     

红树植物拟海桑及其亲本的根际细菌群落特征分析

植物根际微生物群落能够从亲本传递给子代, 从而影响植物的表型。野外调查发现, 海桑属(Sonneratia)红树植物自然杂交杂种拟海桑在野外通常比亲本生长更为强壮, 表现出极强的生存优势。为探究这一现象的原因, 本研究从根际微生物角度出发, 利用细菌16S rRNA基因高通量测序技术, 对采自海南省东寨港的三种红树植物拟海桑(S. × gulngai)及其亲本海桑(S. caseolaris)和杯萼海桑(S. alba)的根际土壤进行根际细菌群落特征分析。结果表明, 三种红树植物根际细菌群落多样性高, 种类丰富, 分属于30门242科351属, 其中变形菌门(Proteobacteria)为最优势门, 在各个样本中丰度超过40%, 子代拟海桑继承亲本的根际微生物多数都属于这一类群。研究发现, 子代拟海桑与两亲本的根际细菌类群组成在门水平存在显著差异, 其中酸杆菌门(Acidobacteria)和放线菌门(Actinobacteria)在子代拟海桑中丰度分别为4.3%和6.5%, 显著高于亲本1%~2%的含量; 在亲本杯萼海桑中, 丰度高达19.8%的栖热菌门(Thermi)在子代中丰度仅有1%, 而热袍菌门(Thermotogae)(5%)甚至消失。总之, 子代拟海桑与亲本杯萼海桑的根际微生物群落组成相对于亲本海桑表现出更大的差异性。土壤理化性质分析发现, 子代拟海桑的土壤全氮(total nitrogen, TN)含量显著低于亲本, 含量相差3倍以上, 相关性分析表明, TN浓度与菌群中的浮霉菌门(Planctomycetes)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)丰度显著负相关。通过功能预测分析发现, 子代拟海桑微生物群落中与基础代谢相关的碳水化合物、氨基酸、能量以及脂质代谢相关的功能基因丰度显著都高于亲本, 表现出代谢能力的增强。本研究认为, 子代拟海桑对亲本的根际土壤微生物进行了选择性继承, 菌群组成更为合理, 在保持菌群高度多样性的同时, 一些根际促生菌的含量增加, 使菌群基础代谢能力增加, 更有利于子代拟海桑的生长。     

气候变化对海洋鱼类群落结构的影响研究进展

全球气候变化影响着海洋生态系统的多个方面,而鱼类群落结构对气候变化的响应机制是探索海洋生态系统演变规律的关键点之一。本文结合国内外相关研究成果,概述了气候变化引起的温度、盐度、CO₂浓度、海平面高度、溶解氧以及海流等的改变对鱼类群落结构的影响,并以太平洋十年涛动(Pacific Decadal Oscillation,PDO)和厄尔尼诺−南方涛动(El Niño Southern Oscillation,ENSO)等典型气候现象为例,探讨了鱼类群落对典型气候现象的响应,讨论了需要解决的重点问题,以期为科学应对气候变化和制定海洋生物多样性保护策略提供依据。     

Blondel和k-核分解混合算法相结合的网络空间点群要素多尺度模型构建

随着人们对网络空间的依赖性不断增强,互联网技术与网络基础设施规模迅速发展。很难直接用数字或表格的形式对网络空间进行全局的规划与管理,并且不容易发现隐藏在网络空间中的一些关键信息。网络空间点群要素的多尺度模型构建对网络空间数据的多尺度分析和可视化具有非常重要的意义。本文以网络空间的特征为依据,在借鉴基于社团划分的网络空间分层算法和基于节点重要性的网络空间分层算法特点的基础上,提出了Blondel算法和k-核分解的混合算法相结合的网络空间点群要素多尺度模型构建算法。本算法通过自动社团划分,用同一社团内的节点合并构建新的网络,有效解决了基于节点重要性的网络空间分层算法自动化程度低的弊端。利用核心节点来代替整个社团结构,显著保留了网络空间中节点的属性。实验表明使用该算法可以使各个层次网络空间点群要素的综合比例降至30%以下,较好的实现了网络空间点群要素的聚类与分层,若将网络空间点群要素的多尺度模型应用于地理空间中,则可实现网络空间地图的多尺度绘制。