舟山海域外来物种地中海贻贝的自然分布现状及生态影响

以筛选的2个可鉴别的地中海贻贝（Mytilus galloprovincialis）、厚壳贻贝（M.coruscus）及其杂交后代的PCR标记（扩增核基因Glu-5’特异片段PCR标记Me14/Me17和延伸因子1α第一个内含子区段PCR标记引物EFbisF/EFbisR）,对舟山海域3个贻贝主产区（马鞍列岛海域、浪岗山列岛海域、中街山列岛海域）采集的贻贝样本的种类进行鉴别.研究结果显示：100个马鞍列岛海域贻贝个体和30个中街山列岛海域贻贝个体均为厚壳贻贝;而54个浪岗山列岛海域贻贝样本中,16个个体（29.6%）为厚壳贻贝,38个个体（70.4%）为地中海贻贝;3个群体中均未检测到2种贻贝的杂交个体.因此,推测舟山海域地中海贻贝自然分布仅局限于局部海区,但在适宜的海区地中海贻贝可取代厚壳贻贝成为贻贝床的优势物种,舟山海域贻贝床的动态变化值得进一步关注;贻贝样本中没有检测到地中海贻贝与厚壳贻贝的杂交个体,表明调查海域可能没有杂交贻贝分布或数量极少,地中海贻贝通过杂交、基因渐渗污染厚壳贻贝基因组的风险较小.     

Uses of invasive alien plant species in Rema-Kalenga Wildlife Sanctuary of Bangladesh

An exploratory survey was conducted on the uses and role of invasive alien species(IAS) to local livelihoods in the Rema-Kalenga Wildlife Sanctuary in the northeastern hilly region of Bangladesh.Vegetation was sampled in 50 quadrates,each 10 m×10 m,and 25 line transects,each 50 m×20 m,through a combination of random and systematic sampling.Group discussions were also arranged with fifty local inhabitants(17 % of the total inhabitants) to learn the various uses of IAS.A total of 17 alien plant species belonging to 10 different families,of which Leguminosae constitutes the highest number(5 species),include tree(53%) followed by herb(12%),shrub(12%) and others(weed,climber and palm).The majority of the identified species were found to be used for fuel,followed by timber production,medicinal or curative uses,fodder,and many others.In addition to providing various forest products,alien tree species planted in forest plantation help provide many vital ecosystem services such as soil amelioration through nitrogen fixation.It is true that the alien species have some negative impacts on local ecosystems,and not all of them are harmful.Therefore,a national programme must be initiated to distinguish the harmful from the harmless species and to identify the uses and impacts of the former and latter.     

Repeat photography as a tool for detecting and monitoring historical changes in South African coastal habitats

Repeat photography was used to illustrate long-term changes occurring in coastal habitats in the Western Cape, South Africa. Historic images were sourced from books and theses, the public and subject specialists, and repeat photographs were then taken from the same perspectives. Visible changes could be categorised into four types: (1) changes in species’ ranges; (2) biological invasions; (3) sea level changes; and (4) direct engineering impacts. In terms of range changes, the images depict a progressive easterly spread of the cold-water kelp Ecklonia maxima and parallel easterly contraction of the warmer-water mussel Perna perna, both evidence for declining water temperatures along the South-West Coast. Since c. 1980 most shores have also become conspicuously invaded by the alien Mediterranean mussel Mytilus galloprovincialis, while those on the West Coast have also been visibly invaded by the more-recently introduced Pacific barnacle Balanus glandula. No changes in vertical zonation due to changing sea levels could be detected, despite suitable images being available. Construction along the shore has radically altered the appearance of the shoreline in urban areas. Repeat photography thus proved a useful tool for both detecting and dramatically illustrating historic changes over the past century. These changes have altered substantially both the appearance and ecological attributes of many rocky shores in this region.     

Differential feeding rates of native and alien predators on the invasive Asian date mussel Arcuatula senhousia (Benson in cantor, 1842) in a Mediterranean coastal lagoon

The alien Asian date mussel Arcuatula senhousia inhabits several coastal environments worldwide. This species can form dense mats where individuals attach through byssus threads, thus altering the structure, functioning and biodiversity of the native communities. We investigated the feeding preference of different predators on this alien species in a Mediterranean coastal lagoon. To do this, a mesocosm experiment was conducted using two crab species (the Mediterranean green crab Carcinus aestuarii) and the Say mud crab Dyspanopeus sayi, and one gastropod, the banded dye-murex Hexaplex trunculus as predators of A. senhousia with or without byssus mats. Our data suggest that C. aestuarii is a more effective predator against A. senhousia than D. sayi, and that H. trunculus is almost ineffective. A possible implication of this result is the potential use of the native crab C. aestuarii for limit the formation of the byssus mats, thus mitigating their potential negative effects on the native communities.     

回顾与展望:关于有害藻华种类休眠体通过船舶压舱水底泥地理扩散的研究

船舶压舱水可引起外来生物入侵,国际海事组织(International Maritime Organization, IMO)因此制定了具有约束力的压舱水公约,已于2019年1月对我国正式生效。大量研究证明船舶压舱泥是有害藻华种类特别是有害甲藻孢囊的携带者。另一方面,有害藻华在我国近三十年频发,尤其是新的种类不断出现。因此,这种态势是否与压舱泥带来的外来种入侵有关是一个具有重要科学和现实意义的问题。我们最近的工作表明,此前国内外通过形态学方法对压舱泥的检测都大大地低估了甲藻孢囊的种类多样性和样品间种类组成的异质性。因此,必须通过更深入全面的实证研究对外源有害藻华甲藻通过压舱泥入侵我国海域进行风险评估。本文从如下几个方面进行了综述与讨论：(1) 船舶压舱水(泥)与有害藻华地理扩散;(2) 国内外有关压舱泥中甲藻孢囊多样性的检测与溯源研究;(3) 我们对美国港口船舶压舱泥的检测发现压舱泥中甲藻孢囊具有很高的种类多样性和样品间的异质性。最后从我们最近的工作及与文献报道的工作比较获得了几个重要的科学问题。在评述国内外研究成果和前期研究结果基础上,提出有必要通过更大量、广泛的国际船舶压舱泥和中国全海域沉积物取样、高通量测序的分子鉴定与形态观察、单孢囊分子-形态鉴定、孢囊萌发、全面生信分析相结合的技术路线,实证性地评估有害藻华种类通过压舱泥入侵中国的风险,并以此建立对新出现藻华种类溯源或判断是否外来种入侵的基本依据,同时为海关职能部门的检验执法提供依据。     

Jonkershoek: Africa's oldest catchment experiment - 80 years and counting

The Jonkershoek Forestry Research Station was established in the Mediterranean climate region of South Africa in 1935 to implement a multiple catchment experiment to determine the effects of afforestation on water yield. The experiment consists of six neighbouring catchments previously supporting indigenous fynbos shrublands, five of which were sequentially afforested with Pinus radiata plantations every 8 years from 1940 to 1980 and one kept as the control. They conclusively demonstrated a significant impact of afforestation on streamflow. The treatment catchments have seen subsequent plantation rotations since 1980 and rainfall and streamflow observations have been continued to date. Here we describe the site, experimental design, rainfall and streamflow records, instrumentation, and how to access the data. We also provide details of recently installed instrumentation, including full weather stations, fog gauges, and an eddy covariance flux tower. The Jonkershoek catchment experiment was the core of a globally significant interdisciplinary research programme (1935–1992) that shaped most environmental policies and practices relating to biodiversity, forestry, fire and water in South Africa and beyond. The South African Environmental Observation Network (SAEON) inherited the experiment in 2010 and is maintaining it as a long-term eco-hydrological research platform and global change observatory.     

Invasion and spreading of Cabomba caroliniana revealed by RAPD markers

Applying randomly amplified polymorphic DNA （RAPD）, the genetic variation of Cabomba caroliniana Gray （cabomba or fanwort）, a new alien plant in China, was analyzed in this paper. Total 143 bands, including 47 polymorphic bands, were amplified from 23 primers in 20 samples. The sampling distance was large, but its genetic diversity was low. The main results were that： （1） Cabomba, which grew and dispersed mainly in fragment, was an abundant and dominant species in freshwater, and its main dispersal mechanism was vegetative reproduction. （2） Cabomba was originally introduced into China as an aquarium submerged plant. Somehow, those discarded cabomba became invasive species in the areas of Hangzhou, Shanghai, and Meicheng, and other places. （3） Although the level of genetic diversity in cabomba was low, their rapid dispersion and propagation could seriously harm to local aquatic community. Therefore, specific measure should be used to control cabomba from uncontrolled spreading and damage to local vegetation communities.     

罗园井田构造特征及发育规律探讨

通过对罗园井田普查、详查、勘探各阶段工作及以往资料的收集、整理,利用计算机成图,分析了该井田含煤地层的赋存形态,研究了井田的构造特征、变化规律,探讨了陡倾斜、缓倾斜断裂块段的辰布规律。对下一步的矿井设计、建设和开采具有一定的指导意义。     

Long-term physical,chemical and biological changes in a small,urban estuary

The Diep River estuary, a small estuary in suburban Cape Town, South Africa, has been subject to disturbance for centuries. Several earlier studies have documented conditions in the system, providing baselines against which to measure more recent changes. This study: (i) describes major physical and hydrological changes that have occurred within this estuary; (ii) documents faunistic changes subsequent to earlier biological surveys; and (iii) provides an up-to-date faunal list. Salinity measurements and both invertebrate and fish samples were taken at five stations along the estuary in summer and winter 2014. A census of sandprawn Callichirus kraussi densities was also undertaken to compare with earlier surveys. Developments within the Diep River catchment and estuary have resulted in extensive changes in flow and salinity regimes, causing marked reductions in summer salinity levels, changes in frequency of mouth closure, and deteriorations in water quality. These have resulted in major changes in faunal composition and distribution, including an increase in numbers of non-indigenous species. Surveys in the early 1950s recorded 47 invertebrate species, whereas only 23 were found in 1974. A total of 23 species were again recorded in 2014, but these included several freshwater forms not previously reported, which had entered the system due to lowered salinity values, as well as new alien introductions. Only six of the 69 taxa recorded were reported by all three surveys. There have been substantial declines in sandprawn abundance, from 40 million in 1998 to just over 12 million in 2014. In all, 12 fish species were recorded in the 1950s, nine in 1974, but only five in 2014, including the newly detected invasive mosquito fish Gambusia affinis and the translocated tilapia Tilapia sparrmanii. Thus, only three of the original native fish species remain. Contrary to these losses, the present bird fauna appear to be more abundant and diverse than previously. Regular monitoring is recommended to obtain a clearer understanding of ongoing changes, and major management interventions will be needed if further degradation is to be prevented.     

斑纹小贻贝的营养成分分析与评价

外来物种是一把“双刃剑”,对本土生态环境和社会经济产生重要影响,准确评估外来物种的营养价值是发挥其经济效益的重要环节。作为近年来侵入我国东南沿海的外来物种,斑纹小贻贝（Mytella strigata）一方面威胁着本地种翡翠股贻贝的生存,另一方面在海南、福建等地区已作为海产品被售卖和食用。在此背景下,本研究依据国家食品安全检测相关标准,对斑纹小贻贝软体部常规营养指标进行测定,分析与评估斑纹小贻贝的营养价值。结果显示：斑纹小贻贝软体部水分、灰分、粗蛋白和粗脂肪的含量（湿重）分别占83.20%、2.00%、12.09%和0.60%;共检测出16种氨基酸,占粗蛋白总量的84.53%,其中7种为必需氨基酸,占氨基酸总量的35.23%,必需氨基酸指数EAAI评分为76.24分,第一限制性氨基酸是半胱氨酸+蛋氨酸;检测出18种脂肪酸,包括7种饱和脂肪酸,占43.71%,4种单不饱和脂肪酸,占11.05%,7种多不饱和脂肪酸,占45.27%,DHA和EPA分别为脂肪酸总量的19.85%和13.32%;矿质元素种类丰富,钙、铁、锌、锰等微量元素含量高。研究表明,斑纹小贻贝软体部氨基酸比例符合FAO/W...