Directional dependence of P- and S-wave propagation and polarization in foliated rocks from the Kola superdeep well: Evidence from laboratory measurements and calculations based on TOF neutron diffraction

We have measured P- and S-wave velocities on two amphibolite and two gneiss samples from the Kola superdeep borehole as a function of pressure (up to 600 MPa) and temperature (up to 600 °C). The velocity measurements include compressional (Vp) and shear wave velocities (Vs1, Vs2) propagating in three orthogonal directions which were in general not parallel to inherent rock symmetry axes or planes. The measurements are accompanied by 3D-velocities calculations based on lattice preferred orientation (LPO) obtained by TOF (Time Of Flight) neutron diffraction analysis which allows the investigation of bulk volumes up to several cubic centimetres due to the high penetration depth of neutrons. The LPO-based numerical velocity calculations give important information on the different contribution of the various rock-forming minerals to bulk elastic anisotropy and on the relations of seismic anisotropy, shear wave splitting, and shear wave polarization to the structural reference frame (foliation and lineation). Comparison with measured velocities obtained for the three propagation directions that were not in accordance with the structural frame of the rocks (foliation and lineation) demonstrate that for shear waves propagating through anisotropic rocks the vibration directions are as important as the propagation directions. The study demonstrates that proper measurement of shear wave splitting by means of two orthogonal polarized sending and receiving shear wave transducers is only possible when their propagation and polarization directions are parallel and normal to foliation and lineation, respectively.     

An innovative priming system - emulsion-based booster

The mineral industry is leading towards a technology driven optimization process. Drilling and blasting are such unit operations in a mine, which can alter the balance sheet of the mine if not planned properly. The development, improvement and utilization of innovative technologies in terms of blast monitoring instruments and explosives technology are important for cost effectiveness and safety of mineral industries.

The ever-growing demand for minerals has compelled the industry to adopt large opencast projects using heavy equipment. This has necessitated use of a few hundred tonnes of explosives in each blast. The bulk delivered fourth generation explosives have solved the problem of explosive loading to a large extent as it provides improved safety in manufacturing, transportation and handling. Bulk delivered emulsion is non-explosive until gasification is complete and a large quantity of explosive can be transported and loaded into blast holes efficiently and with safety. The priming of bulk delivered explosives in Indian mines uses the conventional PETN/TNT-based boosters. The conventional booster possesses safety problems in terms of handling and use, so Indian Explosives Ltd has developed an emulsion-based booster (Powergel Boost).

This paper explores the potential of an emulsion-based booster used as a primer to initiate bulk delivered emulsion explosives used in mines. An attempt has been made at a comparative study between the conventional booster and the emulsion-based booster in terms of the initiation process developed and their capability of developing and maintaining a stable detonation process in the column explosives. The study has been conducted using a continuous velocity of detonation (VOD) measuring instrument, the VODMate two channel system manufactured by Instantel Inc. of Canada. During this study three blasts were monitored. In each blast two holes were selected for study, the first hole was initiated using a conventional booster while the other one used an emulsion-based booster. The findings of the study advocates that the emulsion-based booster is capable of the efficient priming of bulk delivered column explosive with a stable detonation process in the column.     

MALBAC技术扩增微生物群落基因组的效率评估

环境样品的低生物量是微生物宏基因组学研究面临的首要挑战,通过基因组扩增技术来满足高通量测序对DNA样品量的需求是最常用的解决策略。MALBAC(Multiple Annealing and Looping Based Amplification Cycles)基因组扩增试剂盒最初为扩增和研究哺乳动物的单细胞基因组而研发。本文中,我们通过人工构建的微生物群落来检测该试剂盒在微生物宏基因组扩增方面的效率和应用可行性。结果表明,每个标准反应中,10 pg的DNA模板量足以满足MALBAC试剂盒对样品扩增的需要。每个标准反应DNA模板用量为10和100 pg时,所扩增DNA样品的基因组覆盖度与原始未扩增样品表现出高度的一致性,证明MALBAC试剂盒扩增效果的高度稳定性和一致性。常用的GenomePlex全基因组扩增试剂盒使我们可以在每个标准反应DNA模板量为100 pg的条件下扩增获得足够的DNA样品,但是结果表明该参照试剂盒无法有效的实现对群落中低丰度细菌菌株基因组的线性扩增。对于MALBAC试剂盒和参照试剂盒而言,在扩增高GC含量的微生物物种基因组DNA方面效率低下。我们的实验结果表明MALBAC试剂盒在高效扩增环境样品宏基因组DNA方面的可行性,但对该试剂盒在扩增环境样品中高GC含量微生物物种方面的适用性存在疑虑。     

基于SLAF-seq技术的黑棘鲷微卫星标记开发及其在鲷科鱼类中的通用性研究

黑棘鲷(Acanthopagrusschlegelii)是广泛分布于西北太平洋的暖温性中下层经济鱼类,也是我国沿海重要的海洋捕捞鱼类和增养殖对象。然而,目前有关黑棘鲷的微卫星标记研究报道较少,难以对其种质资源状况作出精确评估。本研究采用SLAF-seq技术测序共获得22489个二至六碱基重复的黑棘鲷微卫星序列,短重复序列(二、三碱基)占总微卫星序列的90.8%,长重复序列(四至六碱基)占有9.2%。经过157对随机合成引物的多态性筛选,开发出49个高多态性的黑棘鲷微卫星标记,其中短重复序列位点有25个,长重复序列位点有24个。每个位点的等位基因数(Na)为2—20(均值为8.3),观测杂合度(Ho)和期望杂合度(He)分别为0.097—0.938和0.122—0.922(均值分别为0.663和0.701),多态信息含量(PIC)为0.118—0.897(均值为0.655)。经Bonferroni校正后,有47个位点符合哈迪-温伯格平衡(HWE),各位点间未检测到连锁不平衡现象,仅2个位点偏离HWE。结果表明,所开发的大部分微卫星标记具有高多态性,蕴含的遗传信息含量较为丰富,能够为黑棘鲷的种群遗传资源评估提供数量充足、类型多样的有效分子标记。跨物种扩增结果显示,有43个黑棘鲷微卫星标记可在9种鲷科鱼类中成功扩增,其中28个标记在太平洋棘鲷(Acanthopagruspacificus)、黄鳍棘鲷(Acanthopagruslatus)和澳洲棘鲷(Acanthopagrusaustralis)中具有较好的通用性,2个标记在平鲷(Rhabdosargussarba)、蓝点赤鲷(Pagruscaeruleostictus)、真赤鲷(Pagrusmajor)、二长棘犁齿鲷(Evynnis cardinalis)及黄牙鲷(Dentex hypselosomus)中具有通用性。这些通用性标记可为阐明鲷科属、种间的系统进化关系和棘鲷属鱼类的群体遗传学分析提供新的标记来源和研究角度。     

黄土河谷场地地形放大效应的研究

使用黄土河谷场地模型对地震动峰值加速度和频谱特征进行研究。结果表明,不同工况下各观测点PGA的放大比呈现出不同的变化规律。分析山顶和山谷各点的傅里叶谱以及谱比发现,在地形和土层共同作用下的放大效应是各自单独作用下放大效应的乘积。     

速度差对井间走时层析成像质量的影响及其改进方法

在井间地震初至走时层析成像中,随着相邻地质体速度差的增大,使得射线分布不均匀,以及网格剖分不合适,导致层析成像结果不理想.物理和数值模型的井间走时层析成像表明：当速度差超过300%时,层析结果畸变较大;在300%～150%之间时,层析结果较好;低于150%时,层析结果好.在此基础上,提出了井间多尺度初至走时层析成像方法,即对同一模型采用多种网格剖分来同时进行层析成像,以获得研究区域的速度图像.数学和物理模型的井间多尺度走时层析结果表明：该方法很好地兼顾了层析成像的分辨率和精度,极大地改善了井间地震层析成像的质量.即使速度差超过30%,其多尺度的层析结果仍然较好.因此,这种方法具有实际应用的潜力.     

复杂载荷作用下立管触地段损伤管道的动力响应

触地段(Touchdown zone, TDZ)是在役钢悬链线立管(Steel catenary riser, SCR)的关键部位,在复杂载荷作用下,极易形成损伤缺陷,其载荷寿命的评估是深海结构工程中的一个关键问题。本文以大型有限元软件ABAQUS为平台,运用损伤管道实体单元与土弹簧阻尼单元相互作用的模型模拟触地段损伤海底管道在复杂载荷作用下的动力响应,数值计算考虑了管-土相互作用过程中的材料非线性、几何非线性以及接触非线性。讨论了单一环向体积损伤位于触地段管道的不同位置时,触地段损伤管道在不同载荷作用下的动力特性及特征点的动力响应。结果表明,管道所受内外压力以及管道提升端的竖向位移载荷会影响结构的自振频率;体积损伤部位的动力响应较完好部位更剧烈;体积损伤的位置和动力载荷频率对管道动力放大系数的影响很大;当动力载荷的激励频率越接近结构基频时,损伤管道的动力响应及动力放大系数越大。     

不同光程放大校正因子对中国近海典型水体吸收光谱的影响

在总结各种文献对光程放大校正因子研究的基础上,通过颗粒物吸收系数量值和吸收光谱谱形两个方面,对不同光程放大校正因子对中国近海典型水体颗粒物吸收光谱的影响进行了比较。当ODf-ODf(750 nm)的吸光度在0.005～0.5的范围时,不同光程放大校正因子得到的悬浮颗粒物吸光度值差异较大。     

利用扩增vvhA基因的环介导等温扩增技术快速，特异而敏感地检测创伤弧菌

创伤弧菌是一种可感染人类的河口病原菌。建立快速,特异而敏感的检测方法,有助于创伤弧菌感染的早期疾病诊断和及时治疗。本研究设计了针对vvhA基因的一系列引物（包括两对外部引物和两对内部引物）,采用环介导等温扩增技术（LAMP）来检测创伤弧菌。结果显示,本方法的最适扩增温度是63℃,反应仅需35分钟。扩增产物不仅可以用含有DNA ladder的琼脂糖凝胶电泳检出,也可借助钙黄绿素直接肉眼观察。采用45株菌株检测该方法的特异性,其中所有创伤弧菌均被检出而其他菌株检测结果皆为阴性。本方法的敏感性是普通PCR扩增的100倍,同时利用该方法可以准确检测出所有的模拟样品、临床标本及环境样品。与其他已知方法比较,针对vvhA基因的介导等温扩增技术可以快速、简单、敏感及特异地鉴定创伤弧菌。     

Sensitive and rapid detection of two toxic microalgae Alexandrium by loop-mediated isothermal amplification

A loop-mediated isothermal amplification (LAMP) assay was designed and evaluated for rapid detection of the toxic microalgae Alexandrium catenella and A. minutum, which can produce paralytic shellfish poisoning (PSP). Two sets of four specific primers targeting these two species were derived from the sequence of internal transcribed spacer (ITS) of ribosomal DNA. The method worked well in less than an hour under isothermal conditions of 65℃. LAMP specificity was validated in closely related algae as a comparison, suggesting the strict specificity of the LAMP primers. Two visual inspection approaches were feasible to interpret the positive or negative results. The detection limits of A. catenella and A. minutum samples using the LAMP assay were found to be 5.6 and 4.5 pg DNA, respectively. The sensitivity of this LAMP assay was 10 or 100-fold higher than Polymerase Chain Reaction (PCR) method in detecting the two microalgae. These characteristics of species specificity, sensitivity, and rapidity suggest that this method has the potentiality in the monitoring of red tide caused by A. catenella and A. minutum.