Extensive genetic divergence among Diptychus maculatus populations in northwest China

D. maculates is a kind of specialized Schizothoracinae fish has been locally listed as a protected animal in Xinjiang Province,China. Ili River located in north of Tianshan Mountain and Tarim River located in north of Qinghai-Tibetan Plateau were two main distribution areas of this fish. To investigate the genetic diversity and genetic structure of D. maculates,four populations from Tarim River system and two populations from Ili River system were collected in this study. A 570-bp sequence of the control region was obtained for 105 specimens. Twenty-four haplotypes were detected from six populations,only Kunes River population and Kashi River population shared haplotypes with each other. For all the populations examined,the haplotype diversity(h) was 0.904 8±0.012 6,nucleotide diversity(π) was 0.027 9±0.013 9,and the average number of pairwise nucleotide differences(k) was 15.878 3±7.139 1. The analysis of molecular variance(AMOVA) showed that 86.31% of the total genetic variation was apportioned among populations,and the variation within sampled populations was 13.69%. Genetic differences among sampled populations were highly significant. F st statistical test indicated that all populations were significantly divergent from each other(P 0.01). The largest F st value was between Yurungkash River population and Muzat River population,while the smallest F st value was between Kunes River population and Kashi River population. NJ phylogenetic tree of D-loop haplotypes revealed two main clades. The neutrality test and mismatch distribution analysis suggested that the fish had went through a recent population expansion. The uplift of Tianshan Mountain and movement of Qinghai-Tibetan Plateau might contribute to the wide genetic divergence of D. maculates in northwest China.     

人类活动对海洋生态系统影响的空间量化评价——以莱州湾海域为例

本文针对海域管理中难以量化人类活动对海洋生态系统影响的问题,提出人海关系空间量化模型,通过完善概念模型、构建指标体系、确定权重及标准,最终利用作用强度和位置关系量化人类活动对海洋生态系统的影响,数值在0—1之间,等分为四个影响程度,由弱到强依次为微弱、中等、强烈和极强,并将该模型和量化方法应用于莱州湾海域。研究结果表明:整个莱州湾受人类活动的综合影响均值为0.425,处于中等影响程度;其中污水排放、围填海工程及港口航运是对莱州湾生态系统影响比较强烈的人类活动;研究海域中40%受到人类活动的强烈影响,44%为中等影响,16%为微弱影响。空间量化分析显示,人类活动对莱州湾近岸海域的综合影响比邻近外海强烈,其中影响较强烈的区域出现在西南近岸海区,而影响较微弱的海区则为莱州湾北部外海。该方法空间量化结果与莱州湾实际情况相符,说明人海关系空间量化模型适合定量化评估人类活动对该海域生态系统的影响,可为"山东半岛蓝色经济区"海域管理提供技术支持。     

南渡江河口洪、枯季节水文泥沙变化特征及台风“海鸥”对入海水沙的影响

基于2014年最新的洪、枯季节实测资料,分析了南渡江河口海域水文泥沙的季节性变化特征,借助台风"海鸥"过境期间的河道监测数据,研究了台风天气造成的洪水事件对河流入海水沙通量的影响。研究结果表明:(1)洪、枯季节河口海域的水体层化作用不强,洪季的水温高于枯季,但盐度低于枯季。水温呈现向海递减的趋势,而盐度整体分布较为均匀;(2)河口水体含沙量近岸大于远岸,枯季河口三角洲泥沙向西输运。洪季含沙量明显高于枯季,大量泥沙在台风季节被冲刷入海,而后向海或向西输运扩散;(3)河口海域为不规则全日潮,呈现东西向往复流特征。温度、盐度和浊度均呈现较强的潮汐性变化特征;(4)枯季河道内存在明显的盐水楔,锋面处的垂向梯度很大,在口门向陆大约12—15 km以远的河道水体不再受潮汐影响;(5)台风"海鸥"影响下,南渡江洪峰期间的径流量和含沙量均远远超过多年平均值,反映了南渡江河口地区"台风季节"的特点。     

基于氮稳定同位素的九龙江口鱼类营养级研究

采用氮稳定同位素技术对2013年6—8月九龙江口采集到的隶属于13目42科61属共71种鱼类的营养级进行研究。结果表明,九龙江口鱼类的δ15 N值跨度较大,介于7.23‰~14.66‰之间。以菲律宾蛤仔(Ruditapes philippinarum)为基准生物估算出鱼类的营养级介于1.70~3.89之间,营养级最低与最高的鱼类分别为少牙斑鲆(Pseudorhombus oligodon)与多鳞鱚(Sillago sihama)。与Fishbase同种鱼类的营养级数据进行比较,83.1%的鱼类的营养级均值处于Fishbase同种鱼类的营养级范围内,但81.7%的鱼类的营养级均值比Fishbase同种鱼类的营养级均值略低;与传统的胃含物分析结果比较,58.3%的鱼类的营养级在0.5个营养级的误差范围内。基于氮稳定同位素估算的鱼类营养级较低的原因可能与本研究样品中低龄鱼及幼鱼比例较大有关。与其他方法相比,稳定同位素法在研究体型较小的幼鱼和低龄鱼营养级更具优势。鱼类营养级随其个体大小的变化明显,但存在种的差异性:不同体长的髭缟鰕虎鱼(Tridentiger barbatus)和绿鳍鱼(Chelidonichthys kumu)的营养级跨度较大,分别为1.54和1.43;而鳓(Ilisha elongata)等的营养级则跨度较小,这可能与不同鱼类的食性变化有关。     

Grain size composition and transport of sedimentary organic carbon in the Changjiang River (Yangtze River) Estuary and Hangzhou Bay and their adjacent waters

Surface sediments from the Changjiang River(Yangtze River) Estuary,Hangzhou Bay,and their adjacent waters were analyzed for their grain size distribution,organic carbon(OC) concentration,and stable carbon isotope composition(δ13C).Based on this analysis,about 36 surface sediment samples were selected from various environments and separated into sand(0.250 mm,0.125–0.250 mm,0.063–0.125 mm) and silt(0.025–0.063 mm)fractions by wet-sieving fractionation methods,and further into silt-(0.004–0.025 mm) and clay-sized(0.004mm) fractions by centrifugal fractionation.Sediments of six grain size categories were analyzed for their OC andδ13C contents to explore the grain size composition and transport paths of sedimentary OC in the study area.From fine to coarse fractions,the OC content was 1.18%,0.51%,0.46%,0.42%,0.99%,and 0.48%,respectively,while theδ13C was –21.64‰,–22.03‰,–22.52‰,–22.46‰,–22.36‰,and –22.28‰,respectively.In each size category,the OC contribution was 42.96%,26.06%,9.82%,5.75%,7.09%,and 8.33%,respectively.The OC content in clay and fine silt fractions(0.025 mm) was about 69.02%.High OC concentrations were mainly found in offshore modern sediments in the northeast of the Changjiang River Estuary,in modern sediments in the lower estuary of the Changjiang River and Hangzhou Bay,and in Cyclonic Eddy modern sediments to the southwest of the Cheju Island.Integrating the distribution of terrestrial OC content of each grain size category with the δ13C of the bulk sediment indicated that the terrestrial organic material in the Changjiang River Estuary was transported seaward and dispersed to the Cyclonic Eddy modern sediments to the southwest of the Cheju Island via two pathways:one was a result of the Changjiang River Diluted Water(CDW) northeastward extending branch driven by the North Jiangsu Coastal Current and the Yellow Sea Coastal Current,while the other one was the result of the CDW southward extending branch driven by the Taiwan Warm Current.     

基于HJ-1A高光谱的黄河口碱蓬和柽柳盖度反演模型研究

碱蓬和柽柳是黄河口湿地典型的盐生植物类型,是多种保护珍禽的主要栖息地,具有景观尺度较小、分布广且多混生的特点。应用覆盖黄河口北部潮滩的HJ-1A高光谱遥感影像,基于现场测量的端元光谱和从遥感影像中使用顺序最大角凸锥法(SAMCC)自动提取的端元光谱,应用线性光谱分解法(LSU)、正交子空间投影法(OSP)、匹配滤波法(MF)、最小能量约束法(CEM)和自适应一致估计法(ACE)5种不同光谱解混方法进行混合像元光谱解混,对比两种方法得到的端元光谱分别对碱蓬和柽柳盖度的反演能力,并给出相应的反演模型。结果显示:(1)现场测量端元光谱取得了较好的碱蓬和柽柳盖度反演结果,其中应用LSU方法的光谱解混结果与现场测量盖度的决定系数对于碱蓬和柽柳分别达到了0.88和0.95;(2)两种端元获取方式的光谱解混结果中,LSU和OSP方法均获得了较高的相关性,ACE解混方法的相关性都最低;(3)SAMCC方法提取端元光谱对柽柳的分解结果与现场测量盖度的相关性远高于碱蓬。     

Characteristics and origin of carbon isotopes of n-alkanes in crude oils from the western Pearl River Mouth Basin,South China sea

The quantitative characterization of carbon isotopes of n-alkanes is commonly carried out in organic geochemical studies. Possible controls on carbon isotopes include source organic matter, maturity, fractionation during oil expulsion and migration, and the mixing of different oils. In this study of the origin of crude oils in the western Pearl River Mouth Basin, the influences of all of these factors have been considered in reaching a conclusion. Carbon isotopes of n-alkanes in the crude oils, and the extracts of the two effective source rocks (the Wenchang and Enping formations) in the basin, exhibit clear differences. The Wenchang source rocks have heavy δ¹³C values that remain almost constant or become slightly heavier with increasing carbon number. The Enping source rocks have light δ¹³C values that become lighter with increasing carbon number. Two groups of oils in this area were identified based on the carbon isotopes of the n-alkanes; groupIoils are similar to extracts of the Wenchang source rocks. However, the groupIIoils are different from both the Wenchang and Enping source rocks and the carbon isotopic profiles of their n-alkanes exhibit a “V” feature with increasing carbon number. The results of artificial thermal maturation experiments indicate that, from the early stage to the peak stage of oil generation (with EasyRo between 0.64% and 1.02%), the δ¹³C values of n-alkanes in the pyrolysis oils become heavier by about 3‰ with increasing thermal maturity, but the shape of the carbon isotopic profiles are not significantly changed. Calculated δ¹³C values of n-alkanes in “mixed” artificial pyrolysis oils indicate that the mixture of oils generated from the same source rocks with different maturities could not change the carbon isotopic profile of the n-alkanes, however, a mixing of the Wenchang and Enping oils could give the “V” feature in the profiles, similar to the groupIIoils in this area. The groupIIoils appear to be mixed Wenchang and Enping oils, the latter being the dominant component in the mixture. We conclude that the source organic matter and the degree of mixing are the main factors controlling the carbon isotopic characteristics of n-alkanes in crude oils in the western Pearl River Mouth Basin.     

黄河三角洲滨海湿地沉积环境演化与碳的累积

2007年在黄河三角洲布设一口23.7 m深的浅钻(ZK5),对ZK5进行沉积学观测及原位密度、总碳、有机碳、Al和营养元素实验室测试分析,对ZK5的沉积物性质、有孔虫种类和地层等进行分析,将ZK5自上而下划分为7个沉积环境,揭示了滨海湿地地质演化过程。利用AMS14C测年方法,结合黄河改道的历史,运用历史地理学和沉积地质学综合分析的方法对7个沉积地层进行了年代确定。利用所确定的年代计算了不同沉积环境总碳(TC)和有机碳(Corg)的加积速率。结果表明:TC和Corg与各营养元素及沉积物的沉积速率呈很好的线性相关,沉积物的沉积速率是Corg和TC加积速率的主控因素。还进一步指出,虽然现代黄河三角洲TC、Corg浓度相对较低(质量比分别为9~20 mg/g,1~4 mg/g),但由于其高沉积速率(最高可达60 cm/a),Corg的加积速率高于世界其他高Corg浓度的湿地,特别是浅海湿地(如三角洲前缘),其Corg加积速率可高达2 102 g/(m2·a)。由此,可以断言现代黄河三角洲是很好的碳汇地质体。     

长江口营养盐结构特征及其对浮游植物的限制

根据2013年5月、11月两个航次的调查资料,分析了长江口营养盐浓度及其结构的分布变化,并探讨了营养盐对浮游植物的限制情况。长江口营养盐分布存在季节差异:口门外NO3-N、NO2-N浓度均为春季高秋季低,PO34-P、3SiO2-Si、NH4-N浓度则秋季高春季低,口门内除NO2-N外,NO3-N、PO34-P、SiO23-Si、NH4-N浓度均为秋季高于春季。NO3-N、PO34-P、SiO23-Si浓度从近岸向外海逐渐降低,NO2-N、NH4-N浓度分布规律不明显。NO3-N是DIN的主要存在形态,其占DIN的比例为春季95%、秋季83%。春季、秋季DIN/P均高于16,表现出长江口过量的DIN输入,春季Si/DIN基本小于1,秋季Si/DIN大于1。春季由于硅藻的局部生长使DIN/P异常升高、Si/DIN异常降低,秋季西北部海区受苏北沿岸流影响,呈低DIN/P值和高Si/DIN值分布。受含过量DIN、SiO23-Si的长江冲淡水的影响,春、秋季均表现为PO34-P潜在相对限制。春季由于浮游植物的大量吸收,局部出现PO34-P、SiO23-Si的绝对限制。当同时考虑绝对限制和潜在相对限制时,春季15.38%的站位受PO34-P限制,限制情况较上世纪90年代更为突出。     

长江口九段沙潮沟系统分维研究

以长江口九段沙为研究区,采用2013年8月13日的Landsat-8遥感全色波段得到的九段沙潮沟分布图,利用Horton-Strahler原则将九段沙潮沟进行了分级。然后使用网格法对各个等级的潮沟进行了分维研究,计算了九段沙不同等级的每个潮沟左右两侧的维数值,并结合潮沟的宽度大小,对其维数值进行了比较和分析。研究结果表明,九段沙相同等级潮沟中,潮沟左侧维数的变化范围大于右侧,即潮沟左侧发育的复杂程度大于右侧;不同等级潮沟宽度的最小值变化幅度不大,但潮沟宽度的最大值变化幅度较大;不同等级潮沟宽度的变化和其潮沟左右两侧维数差值变化的相关性较小;不同等级潮沟,无论左侧还是右侧,潮沟维数大小随着潮沟的等级数和潮沟的平均宽度的增大而增大。