土结构性本构模型研究现状综述

土本构模型的建立是一个重要而又复杂的问题,到目前为止,国内外学者们已提出数以百计的土本构模型,诸多文献也对这些模型进行了评述和归纳。然而这些土本构模型多是在扰动土或砂土的基础上发展和建立起来的,它们难以描述由于土结构性引起的各种非线性行为,其计算结果与实际情况相差甚远。天然土体一般都具有一定的结构性,所以有必要建立考虑土结构性影响的土本构模型。针对这个现实,目前有些学者已基于各种理论和方法,提出了一些可以考虑土结构性影响的土本构模型,并得了较好的应用。但在目前的文献中还很少有对土的结构性本构模型研究进行归纳,基于此,本文简要介绍了一下目前土的结构性本构模型研究现状,并提出了这些本构模型在应用中所存在的问题。     

极地产适冷脂肪酶菌株的酶学性质研究

从南北极环境样品中分离到7株产脂肪酶的细菌,经16SrDNA序列分析表明,这些细菌分别属于假交替单胞菌属（Pseudoalteromonas）和嗜冷杆菌属（Psychrobacter）．采用p-NPP法对这7株细菌所产的脂肪酶进行酶学性质研究,结果显示这些酶的最适作用温度在30～40cC、最适作用pH值在7—8之间,在低温下能保持较高的剩余活力,对热敏感,属于适冷脂肪酶．其中假交替单胞菌（Psychrobacter sp．7342）所产脂肪酶具有低温下酶的剩余活力高、有效作用温度和pH范围广、热稳定性较好及对多种金属离子抗性强等特点．该菌株能利用多种单一氮源和碳源产酶,最适产酶温度为25℃．在此基础上进行正交实验分析得到了该菌株的最佳发酵条件为：蛋白胨和淀粉含量各为1．33％,酵母膏含量为0．3％,温度为25℃．     

壳聚糖酶基因在解脂耶氏酵母中的表达及重组酶性质研究

为构建高效表达壳聚糖酶工程菌株,以Microbacteriumsp.基因组为模板扩增壳聚糖酶基因,目的基因与表达载体pINA1317连接构建重组质粒,重组质粒NotⅠ酶切线性化后转化解脂耶氏酵母Y.liPolytica Po1h。所得结果为PCR扩增得到约1000bp的特异性片段,序列分析表明含有壳聚糖酶全长基因,开放阅读框801bp,编码266个氨基酸残基。转化子酶活力为10.4U/mL,是原菌株酶活力的3.15倍。重组蛋白经DEAE-Sepharose FF分离纯化,达到电泳纯,SDS-PAGE显示单一条带,分子量约41kDa。重组酶反应最适温度为45℃,最适pH为5.6,Km和Vmax分别是0.926mg/mL和6.15U/mL。质谱分析酶解产物主要为三糖和五糖。实现了壳聚糖酶在解脂耶氏酵母中的分泌表达,为壳聚糖酶的工业化生产奠定了理论基础。     

壳聚糖/α,β-甘油磷酸凝胶微球的制备及性质研究

以液体石蜡为油相,以壳聚糖/α,β-甘油磷酸凝胶溶液为水相,用乳化法制备了壳聚糖/α,β-甘油磷酸凝胶微球,并对微球形态、粒径分布、溶胀性、蛋白吸附率、溶血率、体外降解率进行了分析。光学显微镜下显示,微球形态圆整,粒径主要分布在100～400μm;扫描电镜显示,微球内部为三维网络状结构,骨架清晰;pH=7.4的PBS缓冲液中干凝胶微球的溶胀率为218.08%,普通壳聚糖微球为70.98%;1和24h测得凝胶微球的蛋白吸附量分别为13.21和15.68μg/g,普通壳聚糖微球分别为3.71×103和4.83×103μg/g;微球的溶血率小于5%,血液相容性良好;体外降解实验显示,壳聚糖的脱乙酰度、黏度以及溶菌酶浓度对壳聚糖/α,β-甘油磷酸凝胶微球的降解性质产生影响。     

Control of Aptian palaeobathymetry on turbidite distribution in the Buchan Graben,Outer Moray Firth,Central North Sea

It is widely recognised that palaeobathymetry is a key control on the distribution of turbidite deposits. Thus, the utilisation of palaeobathymetric surfaces as an input for numerical turbidity current modelling offers a potentially powerful method to predict the distribution of deep marine sands in ancient (subsurface or outcrop) successions. Such an approach has been tested on an Aptian turbidite deposit from the Buchan Graben, UK Central North Sea, where modelled sand distributions could be quality controlled against available well data.     

Sustainable development: Social outcomes of structural adjustments in a South Australian fishery

The assessment of sustainable development is a relatively recent advent in policy and the evaluation of industry structural adjustments. Although the elements of economic and environmental assessment have been relatively well developed and accepted, the effective inclusion of ‘social’ aspects in assessments of sustainable development are still being grappled with.     

Isolation and Characterization of Collagen from Squid(Ommastreoges bartrami) Skin

Collagen of squid (Ommastrephes bartrami) skin was examined in the present study. Histology showed that collagen fiber in the skin was partially cross-linked with muscle fiber. Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were extracted from the skin and characterized. The results of amino acid composition and electrophoretic patterns revealed that ASC and PSC were both type Ⅰ collagen, containing α1 and α2 chains. FTIR (fourier transform infrared spectroscopy) investigations con-firmed the existence of helical arrangements in PSC of squid skin. The denaturation temperature (Td) and shrinkage temperature (Ts)of PSC were 29.4℃ and 52.8℃, respectively.     

Isolation and Characterization of Collagen from Squid(Ommastrephes bartrami) Skin

Collagen of squid(Ommastrephes bartrami) skin was examined in the present study. Histology showed that collagen fiber in the skin was partially cross-linked with muscle fiber. Acid-solubilized collagen(ASC) and pepsin-solubilized collagen(PSC) were extracted from the skin and characterized. The results of amino acid composition and electrophoretic patterns revealed that ASC and PSC were both type Ⅰ collagen,containing α1 and α2 chains. FTIR(fourier transform infrared spectroscopy) investigations confirmed t...     

叠前地震反演技术的进展及其在岩性油气藏勘探中的应用

与叠后地震反演相比,叠前地震反演可得到更丰富的储层信息,可提高储层的描述精度.目前,叠前地震反演主要包括弹性阻抗反演、叠前P波阻抗和S波阻抗联合反演、叠前地震波形反演.文中概述了叠前地震反演各项技术的主要进展及其典型应用实例,提出了叠前地震反演技术在岩性油气藏勘探开发中的应用策略.叠前地震反演技术在未来的油气勘探开发中将有广阔的应用前景.     

The control of maturity and kerogen type on quantitative analytical pyrolysis data

Quantitative pyrolysis-gas chromatography has been performed on 96 kerogen samples isolated from 17 wells on the Norwegian Continental shelf. Petrographic and bulk geochemical measurements were also performed on the samples, and a combined data set of 117 variables for each sample was analysed using principal components analysis (PCA). This approach provides an objective and reproducible means of kerogen characterisation, which can be easily automated. In addition to objective kerogen characterisation and facile visualisation of facies and maturity related chemical trends, the method has the potential to allow objective prediction of key geochemical parameters such as maturity level from pyrogram data.