Characterization of Turbot(Scophthalmus maximus) Skin and the Extracted Acid-Soluble Collagen

The biochemical composition of the turbot skin was investigated. The moisture level of the skin was found to be 51.4%. Based on dry matter content, there were relatively high protein(82.1%) and lipid(13.1%) concentrations in the turbot skin. Mineral element analysis revealed that the turbot skin had high Ca content(2069.0 mg kg^-1), and the concentrations of toxic heavy metals Hg and Pb were less than 0.005 mg kg^-1, which indicates that the turbot skin is a safe resource for collagen production. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) analysis showed that acid-soluble collagen(ASC) extracted from the turbot skin was type-I collagen. The imino acid content of the ASC was 241.6 per 1000 amino acids, which suggests a relatively high denaturation temperature. The Fourier transform infrared(FTIR) spectra of ASC reflected a highly stable structure, and the measured denaturation temperature of ASC was 29.5℃, which is higher than those from many temperate fishes. ASC was the most soluble at pH 4.0, and when the pH values were below or above 4.0, the solubility decreased rapidly. The ASC exhibited a relatively high solubility when NaCl concentration was lower than 2%. These results show that turbo skin can be employed as a source for producing collagen with high quality.     

Isolation of protein from Chlorella sorokiniana CY1 using liquid biphasic flotation assisted with sonication through sugaring-out effect

Microalgae,a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical,bioenergy and food applications.This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga,Chlorella sorokiniana CY1 which was assisted with sonication.A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides,glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein).The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases,as the yield of protein increased to 77%.The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well.The optimum protein productivity was obtained with 10s of resting time in between sonication.Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode.The optimum condition for protein extraction were found as followed:200g/L glucose as bottom phase with volume ratio of 1:1.25,10s of resting time for ultrasonication,5s of ultrasonication in pulse mode and 0.25g of biomass weight.The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future.     

结合深度学习和图割法的遥感影像建筑物检测

提出一种基于卷积神经网络和图割法的自动提取高分影像建筑物的方法。首先,通过卷积神经网络定位与检测建筑物的位置,逐一提取单个建筑物轮廓,利用检测结果分别建立建筑物和非建筑物的高斯混合模型（GMM）,然后结合最大流最小割的图像分割方式实现全局优化,完成建筑物初步提取,最后用形态学进行优化。通过试验证明了该方法的可行性。     

异源多分辨率卫星影像匹配算法研究

以异源多分辨率遥感卫星影像为研究对象,对影像特征提取、特征描述、特征匹配及匹配提纯算法进行了较为深入的研究;并将不同的特征提取与匹配算法进行组合,通过试验对比得出了各种算法的适用性。在此基础上,设计了一套面向异源多分辨率卫星影像的匹配流程。     

多时相阴影轮廓差分辅助下的城市水体提取

如何避免水体提取中阴影信息与水体信息的混淆,是利用遥感数据提取城市水体信息需要解决的一个问题。本文以高分一号WFV图像及Landsat8 OLI图像为数据源,利用阴影轮廓的位置与形状在不同太阳高度角及太阳方位角下的差异性,提出一种基于多时相阴影轮廓差分的城市水体提取方法(WMSD)。以广州市天河区为试验区进行水体信息提取,同时运用NDWI、MNDWI及SWI指数法分别提取水体信息,进行精度对比分析。结果显示,本文所提出的WMSD方法分类精度超过88%,较NDWI法、SWI法及MNDWI法的水体提取精度分别提高了8.50%、9.50%及4.67%。说明基于阴影轮廓位置与形状的差异提取水体信息的方法能够较好地解决阴影与水体提取信息混淆的问题,为利用遥感数据提取城市地区水体提供了一个可行的处理方法。     

运用人工鱼群算法的3D扫描碎片重建探究

针对传统三维碎片拼接匹配过程中依赖单一特征及存在误差累积的问题,提出了一种运用鱼群算法的全局最优匹配方法。该方法先对碎片点云数据进行多特征提取,结合纹理、专家经验信息对混合在一起的多种类型碎片进行粗糙集分类,之后采用鱼群算法的最优解求得最佳匹配方案。实例验证所提全局匹配方法具有能力强、与初始位置无关及较强的稳健性等特点,为三维碎片的全局匹配提供了一种有效的解决方案。     

大面积水深异常检测的条件变化自编码算法

针对大面积海底地形数据缺失或异常的复杂及多变性特点,结合条件变分自编码器(CVAE)与深度卷积生成对抗网络(DCGAN),构建了条件变分自编码生成对抗网络(CVAE-GAN)大面积海底伪地形的检测与剔除方法。本文方法利用条件变分自编码算法改变原有的样本分布,通过对训练样本的学习重新构建样本之间的分布规律,有效提高了高维到低维映射的稳定性;结合生成对抗网络,提高了整体算法的稳健性,最终得到较优的检测与剔除结果。采用水深格网数据进行试验,并与中值滤波法、趋势面滤波法进行比较。结果表明,本文方法在精度、稳定性及噪声稳健性方面有所提高,验证了本文方法在海底地形数据处理上具有可行性。     

浅析如何在溶剂萃取机理研究中正确使用斜率法

斜率法是萃取机理研究最为常用的方法之一。尽管其理论基础和使用方法非常简单,但该方法却常常被误用,导致其非但不能有效的确定萃取机理,反而产生了误导性结论。本文以t-BAMBP萃取碱金属离子(K~+、Rb~+、Cs~+)体系为例讨论了常见的错误用法以及造成这类情况的根源问题,之后对其他萃取体系中类似错误用法进行了简要评述,表明斜率法的错误使用主要源于对萃取反应方程式的错误假定及对工作方程推导过程中引入的近似处理理解不当。最后总结了在萃取机理研究中正确使用斜率法的几点注意事项。     

番红砗磲外套膜色素提取条件优化及成分分析

以番红砗磲(Tridacna crocea)为实验对象,通过单因素对比实验对其外套膜色素的提取条件进行了优化,并利用薄层层析色谱法对色素组成进行了初步分析。结果表明:丙酮和乙醇为适宜的番红砗磲外套膜色素提取剂;使用丙酮为提取剂时,浸提时间为4 h,提取温度为25℃,料液比为1︰40。使用乙醇为提取剂时,浸提时间为4 h,提取温度为30℃,料液比为1︰200;利用薄层色谱法分离外套膜颜色为蓝色和红棕色的番红砗磲外套膜色素后发现,蓝色番红砗磲外套膜色素由9种色素组分构成,红棕色个体番红砗磲外套膜色素由10种色素组分构成。