基于线粒体基因的石珊瑚分子系统学研究

以广东徐闻地区常见的6科10属17种石珊瑚的21个样本为研究对象,对线粒体COⅠ、16SrRNA和mtSSU三基因片段数据进行了联合分析,并计算了属间和科间遗传距离;利用邻接法、最大简约法和最大似然法分别构建了分子系统树。序列分析结果显示,石珊瑚线粒体基因碱基构成同样具有AT偏倚特征;石珊瑚属间的遗传距离显著大于属内遗传距离,而所有石珊瑚与两个外类种群的距离均大于石珊瑚之间的遗传距离;在系统发育树中,分子系统分类结果与形态学分类阐述的遗传进化关系略有差别,暗示传统形态学分类可能受珊瑚骨骼生长可塑性限制。     

Molecular iodine (I2) emission from two Laminaria species (Phaeophyceae) and impact of irradiance and temperature on I2 emission into air and iodide release into seawater from Laminaria digitata

Kelps of the genus Laminaria accumulate iodine at high concentrations, but the iodine retaining capacity can be affected by emersion and physiological stress. In this study, I₂ emission into the atmosphere from Laminaria digitata and Laminaria hyperborea was compared under controlled low irradiances and temperatures. The two species exhibited different I₂ emission rates as blades of L. digitata emitted I₂ at rates five times higher than those from newly-grown blades (current growth season) of L. hyperborea. I₂ emission was not detectable from old blades (previous growth season) of L. hyperborea. Additionally, effects of irradiance and temperature on both I₂ emission into air and net I⁻ release into seawater where assessed for L. digitata while monitoring photo-physiological parameters as stress indicators. Irradiances between 30 and 120 μmol photons m⁻² s⁻¹ had only marginal effects on both I₂ emission and I⁻ release rates, but physiological stress, indicated by photoinhibition, was observed. The results suggest that the irradiances applied here were not stressful enough to impact on the iodine release. By contrast, at elevated temperatures (20 °C), photoinhibition was accompanied by an increase in I₂ emission rates, but net I⁻ release rates remained similar at 10–20 °C. High I₂ emission rates into air and I⁻ release into seawater observed from L. digitata underpin the fundamental function of this kelp as mediator of coastal iodine fluxes.     

港池泊稳的能量平衡方程数值模拟

对基于能量平衡方程的多向随机波浪传播数学模型进行改进,通过模拟不同防波堤绕射引起的港池泊稳,验证模型的合理性和有效性。利用非线性弥散关系提高模型计算浅水变形的精度;采用二次逆风差分格式离散控制方程,避免了加入绕射项引起的数值耗散;并将文氏谱加入模型中,使其更加适合中国海域的工程应用。应用改进后模型绘制的双突堤和岛式防波堤绕射系数图与我国《海港水文规范》图进行了对比。对比结果十分接近,可以较好地描述港池的泊稳状况,为综合计算波浪在近岸的浅水变形、折射、绕射、反射和能量耗散等作用奠定了基础。     

强迫摇荡运动三维球体水动力系数的CFD计算

采用计算流体力学(CFD)的雷诺平均法(RANS方法),并考虑了自由表面的影响(采用VOF方法模拟自由表面),来模拟三维球体的单自由度强迫摇荡运动,得到纵荡、升沉及横摇的附加质量与阻尼系数。该数值模拟结果与三维势流理论计算的结果进行比较,趋势基本一致,但CFD方法更能凸显粘性的作用。本文所应用方法能合理给出浮式结构物的水动力系数,更精确描述海洋浮式结构物周围的流场,可应用于船舶与海洋工程浮式结构物的水动力性能研究。     

Vertical zonation and aggregated distribution of the Manila clam on subtidal sand flats in a coastal brackish lagoon along the Sea of Japan

The Manila clam, Ruditapes philippinarum, has maintained small‐sized populations in a semi‐enclosed brackish lake along the Sea of Japan, the Honjo area of Lake Nakaumi, although the environment and biota of this area have changed dramatically due to a large‐scale reclamation project. There should be underlying processes that enable the restoration of this species from small‐sized populations, such as the existence of source (i.e. reproductive) populations in other areas and depth zones of the lake. However, there has been no robust, properly designed evaluation of the distribution of the Manila clam in the subtidal sand flats. In order to elucidate the possible mechanisms that allow for the persistence of populations of the Manila clam, we examined the spatiotemporal and vertical variation in distributions of 0‐age clams in the subtidal zone of sand flats. Seasonal effects on population variations showed erratic changes among depth zones without a decreasing trend along the depth gradient. Further, many local populations became extinct even in the shallower zones due to seasonal (summer) hypoxia at deeper zones and hypoxia by the accumulation of key benthic species (Asian mussel and decaying macroalgae) in mats at shallower zones. A few surviving local populations were stable with a spatial‐fragmental (patchy) distribution, associated with fragmented accumulations of Asian mussels and macroalgae. Efforts to maintain stable populations and to restore this species in the subtidal area may depend on these small, restricted, patchy local populations. These findings suggest that high fertility and productivity of the Manila clam as well as patchy distribution of small populations may contribute to the maintenance of the population and the avoidance of extinction (by spatially diffusing the risk of extinction) in harsh environments resulting from the reclamation project.     

万米级深海陶瓷耐压结构水下内爆流场数值模拟

耐压结构是深海潜器的重要组成部分,但在深海的高压环境中却存在内爆的风险。为研究陶瓷耐压结构水下内爆的流场特性,使用针对可压缩多相流问题开发的开源代码,采用直接数值模拟,应用自适应直角网格,对两种压力条件下的耐压结构水下内爆进行了数值模拟。通过低压模拟结果与理论解和试验值比较,验证了模拟方法的有效性,进而开展万米级深海陶瓷耐压结构水下内爆模拟。分析发现：陶瓷耐压结构发生内爆后,其内部气腔存在多次压缩—反弹现象,深海环境压力越大则反弹越不明显;气腔反弹阶段,在结构外部将产生数倍于深海环境压力的冲击波,且传播速度接近声速;冲击波压力峰值与到球心距离呈负指数幂函数关系;在相同深海环境压力下,耐压结构外部监测点的冲击波压力与球体半径呈正比例关系。     

龙口湾水动力特征及其对人工岛群建设的响应

基于龙口湾及附近海域的水文实测资料,利用Mike21数学模型模拟了人工岛建设前后的潮流、波浪、纳潮量及水交换率等水动力特征,探讨了人工岛群建设对龙口湾水动力环境的影响。结果表明,人工岛建设显著改变了龙口湾潮流场特征及水体运动路径,湾内受到人工岛的阻挡,流速普遍减小,局部区域潮流运动形式由往复流变为旋转流,流向变化较大,余流形成多个涡旋;湾外由于堤头挑流作用导致局部区域流速增大且余流流速增大,潮流运动形式未发生明显改变。受人工岛的掩蔽作用,人工岛及附近区域的波浪有效波高普遍减小。龙口湾潮位出现北部最大潮差变小、南部最大潮差增大的格局,壅水作用导致人工岛内部水道潮差变化明显。人工岛建设直接占据了龙口湾海域面积,导致其纳潮量明显减小,水交换率呈现南部和北部增大、人工岛北侧以及内部水道减小的特征,人工岛造成的水动力环境的改变是影响水交换率变化的主要原因。人工岛群建设导致龙口湾内的潮流、波浪、纳潮量以及水交换等水动力特征减弱,是引起龙口湾水动力条件变化的根本因素。     

波浪作用下锚链系泊浮式防波堤动力响应的数值模型研究

浮式防波堤充分利用波能在水深方向的分布特性,在满足工程消浪要求的同时对海域水沙交换影响较小,且能够快速布置,在某些实际工程有一定应用前景。为了深入了解波浪作用下浮式防波堤的动力响应,基于OpenFOAM标准求解器olaFlow,在刚体运动求解计算中植入锚链求解模块MOODY(mooring cable dynamics),实现了基于重叠网格方法的浮体运动与锚链受力耦合求解,建立了锚链系泊浮式防波堤动力响应的二维数值模型。利用该数值模型对锚链系泊单方箱浮式防波堤在波浪作用下的透射系数、运动响应、锚链张力进行了模拟,并和相关试验结果进行了比较。结果表明,模型能够准确模拟二维波浪和浮式防波堤的相互作用,并用于三维模型的改进。     

Does the Port 2000 harbour construction have an effect on the Seine estuary suprabenthic community?

Using a Before/During/After protocol, the effects of Port 2000 (the Le Havre harbour extension) on the suprabenthic communities in the Northern Channel of the Seine estuary (English Channel) were examined from March 2001 to March 2009. Since the beginning of Port 2000 construction in 2002, there have been several changes in the hydrological and sedimentary characteristics in the North Channel, including changes in current velocity, bottoms salinity and sand accumulation, as well as in the benthic communities themselves, moving from mud to medium and coarse sand communities. For the suprabenthos, changes were moderate, corresponding to an increase in species richness in the upper-part of the North Channel and a decrease in species density in the dominant mysids species (i.e., mainly Mesopodospsis slabberi) over time. Due to hydrological changes since the beginning of the Port 2000 project (several years of low freshwater input) it has been difficult to attribute the biological changes to Port 2000 construction only. In the future, the results obtained for the suprabenthos will have to be compared to those obtained for the macrobenthos. Similarly, since the suprabenthos is known to play an important role in the estuarine trophic chain, it will also be essential to estimate the fish distribution in the North Channel and to examine their stomach contents to evaluate the probable changes since the Port 2000 project began.