吉尔吉斯斯坦伊塞克湖一处土壤样品细菌多样性

采用纯培养方法从吉尔吉斯斯坦共和国伊塞克湖一处温泉旁的土壤中分离到32株细菌,通过ARDRA 分析后得到不同的类群,从各个类群中随机选取1个代表菌株进行16S rDNA 序列测定和系统发育的分析。结果表明：分离的菌株分布在假单胞菌属（Pseudomonas）、类芽孢杆菌属（Paenibacillus）、芽孢杆菌属（Bacillus）、寡养单胞菌属（Stenotrophomonas）、Lysinibacillus、溶杆菌属（Lysobacter）、短芽孢杆菌属（Brevibacillus）、根瘤菌属（Rhizobium）、变形杆菌属（Proteus）等9个属,且部分菌株的16S rDNA序列同源性低于97%,可能是潜在的新种。结果揭示了该样点可培养细菌种群的多样性及独特性。     

黑潮源区沉积物古菌多样性调查及研究

为了研究西太平洋黑潮源区不同深度沉积物中古菌群落结构的多样性,应用16S rDNA文库技术,对IMAGES XIV航次采集的岩芯MD06-3047进行基因文库的构建和分析,得到260个克隆,经分析处理后得到56个OTUs(Operational Taxonomic Units).基于16S rRNA序列的同源性比较,绘制系统进化树,进行统计学分析.研究结果显示,西太平洋黑潮源区的本哈姆高原(Benham Rise)沉积物中有丰富多样的古菌群落,在1m内的沉积物中分布着9种古菌类群.古菌序列归属于泉古菌(Crenarchaeota)和广古菌(Euryarchaeota),并以泉古菌为主(占总序列的91.5％).其中,泉古菌包含MGI、MBG-B、MCG和UCII-b 4个类群,并且主要由MGI构成(占总序列的40.8％),而广古菌包含MBG-D、SAGMEG、DHVEG、MBG-E和VAL Ⅲ 5个类群,各类群所占总序列比例均较低(占总序列的0.4％～5.4％之间).研究结果对于理解西太平洋黑潮源区沉积物中古菌多样性有重要意义,并为今后研究古菌群落与环境之间的关系提供科学依据.     

Spatial and temporal changes in microbial diversity of the Marmara Sea sediments

Spatial (10 different locations) and temporal (2 years) changes in characteristics of the Marmara Sea Sediments were monitored to determine interactions between the chemical and microbial diversity. The sediments were rich in terms of hydrocarbon, nitrate, Ni and microbial cell content. Denitrifying, sulfate reducing, fermentative and methanogenic organisms were co-abundant in 15 cm below the sea floor. The local variations in the sediments’ characteristics were more distinctive than the temporal ones. The sulfate and nitrate contents were the main drivers of the changes in the microbial community compositions. N and P were limited for microbial growth in the sediments, and their levels determined the total cell abundance and activity. Seasonal shifts in temperatures of the shallow sediments were also reflected in the active cell abundances. It was concluded that the Marmara Sea is a promising ecosystem for the further investigation of the ecologically important microbial processes.     

Direct PCR amplification of the 16S rRNA gene from single microbial cells isolated from an Antarctic iceberg using laser microdissection microscopy

Here, we describe a technique that allows the genetic linage analysis of 16S rRNA genes in bacteria observed under a microscope. The technique includes the isolation of microbial cells using a laser microdissection microscope, lysis of the cells, and amplification of the 16S rRNA genes in the isolated cells without interference by bacterial DNA contamination from the experimental environment or reagents. Using this technique, we successfully determined 15 16S rRNA gene sequences in cells isolated from an Antarctic iceberg. These sequences showed 94%–100% identity to their closest strains, which included bacteria that occur in aqueous, marine, and soil environments.     

两株产铁锰氧化酶细菌的鉴定及系统发育分析

从福建紫金山铜矿矿井水及连城锰废矿坑泥石样中分离到两株具氧化Fe^2＋和Mn^2＋能力的细菌,分别命名为4-05和11-02,对其进行了形态特征、生理生化特性及16SrDNA序列分析．结果显示菌株4-05为假单胞菌属中的一个种．菌株11-02为不动杆菌的一个种．这两株菌的16SrDNA序列已被GenBank数据库收录,序列号分别为FJ805431和FJ8054322．本实验为后续进一步研究地下水的微生物治理奠定了基础．     

南极菲尔德斯半岛表层土壤样品中细菌多样性的系统发育分析

通过PCR结合变性梯度凝胶电泳(DGGE)技术对从南极长城站附近表层土壤样品中获得16SrDNA序列特征片段V3区序列进行分离。对其中的主要12条DGGE条带进行胶回收,获得的DNA片段经测序以及计算机比对分析发现,它们分别属于β、γ、δ-变形细菌(Proteobac-teria)、噬纤维菌-屈挠杆菌-拟杆菌(Cytophaga-Flexibacter-Bacteroides,CFB)群细菌、放线细菌(Actinobacteria)、蓝细菌属(Cyanobacteria)、酸杆菌属(Acidobacteria)和绿屈挠菌属(Chlo-roflexi)等系统分类群。南极表层土壤样品中的大部分16S rDNA序列与从其他土壤或沉积物样品中直接获得的序列相似性较高(93%-100%)。     

Two stages of isotopic exchanges experienced by the Ertaibei granite pluton, northern Xinjiang, China

¹⁸O/¹⁶O and D/H of coexisting feldspar, quartz, and biotite separates of twenty samples collected from the Ertaibei granite pluton, northern Xinjiang, China are determined. It is shown that the Ertaibei pluton experienced two stages of isotopic exchanges. The second stage of¹⁸O/¹⁶O and D/H exchanges with meteoric water brought about a marked decrease in the δ¹⁸O values of feldspar and biotite from the second group of samples. The D/H of biotite exhibits a higher sensitivity to the meteoric water alteration than its¹⁸O/¹⁶O. However, the first stage of¹⁸O/¹⁶O exchange with the¹⁸O-rich aqueous fluid derived from the dehydration within the deep crust caused the Δ¹⁸O_{Quartz-Feldspar} reversal. It is inferred that the dehydration-melting may have been an important mechanism for anatexis. It is shown that the deep fluid encircled the Ertaibei pluton like an envelope which serves as an effective screen to the surface waters.     

青岛多种天气下生物气溶胶中细菌群落特征

为探究雾、霾、沙尘等多种天气下生物气溶胶中细菌群落结构特征,于2017年12月-2018年4月采集了青岛晴天和多种特殊天气下的大气生物气溶胶样品,运用16 S rRNA高通量测序方法分析了样品中细菌群落结构.结果表明,在雾、霾、沙尘天均检测到对人体健康存在潜在危害的条件致病菌,其中变形菌门(Proteobacteria...     

基于卫星遥感的临沂市冬小麦种植时空变化特征分析

以临沂地区为研究区,基于Terra/MOD13Q1遥感植被数据产品,通过分析冬小麦的遥感光谱特征、物候历特征及其生物学特性,提取2009—2019年临沂地区冬小麦种植信息,利用GIS空间分析方法和统计分析方法分析了临沂冬小麦种植的时空分布变化规律。结果显示:1)空间上,近10年来临沂地区冬小麦种植面积在2 800～3 000 km~2之间浮动,占整个地区土地面积的17%左右,总体变化较小,种植区域呈南多北少的分布格局,主要集中在兰陵、临沭和郯城; 2)从冬小麦种植稳定度上看,连续种植冬小麦10 a及以上的区域,主要集中在兰陵和郯城,面积为1 638 km~2,稳定度高,连续种植小于4 a的区域有1 914 km~2,主要分布在莒南和费县,种植不稳定; 3)时间上,冬小麦面积由2009年的2 838 km~2增加到2019年的2 985 km~2,变化幅度和年均变化率分别为5.5%和0.5%,呈缓慢增加趋势。本研究为临沂地区冬小麦种植的深入了解以及农业结构调整提供了较为准确、科学的参考依据。     

壳聚糖酶高产菌株的筛选和发酵条件的研究

从采集的土样中分离到1株产壳聚糖酶能力较强的菌株OU01,并对OU01进行了16S rDNA序列、形态及生理生化鉴定分析。16S rDNA序列分析表明该菌属于微杆菌属;该菌的形态特征与生理生化鉴定结果表明该菌与Microbacteriumsp.的相似性最高;因此将其初步鉴定为微杆菌属(Microbacterium)。同时对该菌的发酵条件进行了初步研究:该菌的最适培养基组成为(g/L):chitosan 10,(NH4)2SO420,MgSO4.7H2O 1.3,K2HPO4.3H2O 1.4,Glucose 1,Yeast extract 3,NaCl 5,起始pH=6.30。最适发酵温度为30℃,最佳发酵时间为96 h,在上述最优条件下,该菌株产酶达到118 U/mL。Microbacteri-umsp.OU01菌株为壳聚糖酶的研究和应用提供了新的来源。