Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITS sequence analysis

Abstract-The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associatedwith their colonial stages. In this study we applied molecular approaches to analyze the genetic variationof Phaeocystis globosa and Phaeocystis pouchetii from several geogrtaphic regions, and to assist in tracingthe dispersal of bloom-forming Phaeocystis species in coastal waters of China. The Sequences of the inter-nal transcribed sacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystisstrains were determined. Sequence comparison shows that P.globosa was the most divergent toP. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences ex-isted between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearlyshows that the species isolated from the southeast coast of China is identified as P. globosa rather thanP. cf. po     

GIS在全国江河堤防数据管理系统的应用

利用GlS技术，开发了全国江河堤防数据管理系统，方便、灵活地对江河堤防数据进行可视化管理，提高了工作效率。     

山东半岛东部诸岛水域叶绿素—a含量和初级生产力

据１９９０．１１－１９９１年８月期间，山东半岛东部诸岛水域调查资料，分析了该水域叶绿素－ａ含量的时空分布和初级生产力的分布与变化，此分布与该水域的温度和营养盐水含量密切相关。叶绿素－ａ含量的季节变化分三种类型，年变幅为０．１１－１２．８１ｍｇ／ｍ＾３，年平均值为１．１７ｍｇ／ｍ＾３。初级生产力夏季〉春季〉秋季〉冬季，年变幅为２３．００－７９１．６０ｍｇ．ｃ／ｍ＾２．ｄ年平均为１５２．０ｍｇ．ｃ／ｍ     

Spatial Variation in the Structure of Kelp Forest Communities Along a Wave Exposure Gradient

Abstract. Four sites were sampled in kelp (Macrocysiis pyrifera) forests occupying rocky bottom habitats along a wave exposure gradient in central California. Consistent betwecn-site differences were found in the three major structural elements - the surface canopy, the undcrstory assemblage, and the ground cover/turf assemblage - of kelp forest communities. Macrocysiis pyrifera was found at all four sites. Nereucyslis tuelkeana only at the most exposed site. The understory kelps Laminaria setchellii and Pterygopltora californica were also characteristic of exposed sites. Articulated coralline algae were more abundant at exposed sites than protected, while fleshy red algae showed the opposite pattern. All four study sites were located along 8.5 km of coastline, and thus were assumed to have available to them the same species pool for colonization. The substrate composition was the same and the amount of unconsolidated substrate was similar at all four sites. We suggest that exposure to wave-generated water motion, through its influence on the surface canopy and therefore on the amount of light reaching the bottom, is responsible for these between-site differences.     

Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

渤海湾天津近岸海域水环境空间变异分析

由于海岸带的地理条件和海洋动力特性的复杂性以及人类开发活动对海岸带的影响,近岸海域的生态环境系统在空间分布上有很大的变异性.基于现场实测资料,建立系统的空间变异分析模型,对海岸带生态环境空间变化规律进行分析,可以充分了解不同区域的污染特征和变化趋势.应用所构建的空间变异分析模型对渤海湾天津近岸海域的水环境特性进行分析,结合对应年份的入海流量及其变化,分析了天津渤海湾海岸带污染的主因子和区域水环境的分布特征,结果可以对海洋功能区的划分和水环境综合评价结果进行验证和补充.     

Species composition and spatial distribution of euphausiids of the yellow sea and relationships with environmental factors

We investigated species composition and spatial distribution of the euphausiid community in the Yellow Sea and identified the relationship with environmental factors (temperature, salinity, chlorophyll a, nitrate, phosphate, and silicate) using bimonthly data from June, 1997 to April, 1998. The environment varied during the sampling period. In warm seasons, thermocline was well developed rendering lower temperature and higher salinity and nutrient concentrations in the bottom layer. During cold seasons the water column was well mixed and no such vertical stratification was noted. Horizontal distribution of temperature, however, differed slightly between near-coast and offshore areas because of the shallow depth of the Yellow Sea, and between southern and northern areas because of the intrusion of water masses such as Yellow Sea Warm Current and Changjiang River Diluted Water. Four euphausiid species were identified:Euphausia pacifica, E. sanzoi, Pseudeuphausia sp. andStylocheron affine. E. sanzoi andS. affine were collected, just one juvenile each, from the southern area in June and December, respectively.Pseudeuphausia sp. were collected in the eastern area all the year round except June.E. pacifica occurred at the whole study area and were the predominant species, representing at least 97.6% of the euphausiid abundance. Further, the distribution pattern of the species was varied in regards to developmental stages (adult, furcilia, calyptopis, egg). From spring to fall,E. pacifica adults were abundant in the central area where the Yellow Sea Bottom Cold Water prevailed. Furcilia and calyptopis extended their distribution into nearly all the study area during the same period. From late fall to winter, adults were found at the near-coastal area with similar pattern for furcilia and calyptopis. The distribution pattern ofE. pacifica was consistent regarding temperature, salinity, and three nutrients during the sampling period, whereas chlorophyll a showed a different pattern according to the developmental stages. The nutrients should indirectly affect via chlorophyll a and phytoplankton concentration. With respect to these results, we presented a scenario about how the environmental factors along with the water current affect the distribution ofE. pacifica in the Yellow Sea.     

Regional variation in springtime ichthyoplankton assemblages in the northeast Pacific Ocean

The coastal regions of the northeast Pacific support large, economically valuable fishery resources and provide nursery areas for many fish species. Over the last few decades, there have been dramatic shifts in species abundance and composition in this area. In this paper, we examine the springtime spatial patterns in the ichthyoplankton of three oceanographically different regions, the Southeast Bering Sea, the Gulf of Alaska and the U.S. West Coast. The data examined are a subset of a larger database (comprising data from cruises conducted from 1972 to 1997) that is being used to investigate spatial, seasonal and interannual patterns in ichthyoplankton of the northeast Pacific in relation to environmental conditions. Ichthyoplankton were collected during seven cruises using 60-cm bongo nets. Spatial patterns of ichthyoplankton were examined using both classification and ordination techniques. Relative Bray-Curtis dissimilarity coefficients calculated from the log₁₀ (n+1) of abundance data were used as input to the numerical classification of species and stations. Nonmetric multidimensional scaling was also applied to the abundance data to examine geometric patterns in the data. The numerical analyses of the species abundance data sets for each cruise revealed spatial patterns in the ichthyoplankton that suggest the occurrence of geographically distinct assemblages of fish larvae in each region. For all three sampling regions, the assemblage structure is primarily related to bathymetry, and Shelf, Slope, and Deep-Water assemblages are described. This shallow to deep-water gradient in species occurrence and abundance reflects the habitat preference and spawning location of the adult fish. Another degree of complexity is superimposed on this primary assemblage structure in each region and seems to be related to local topography and the prevailing current patterns. The patterns in ichthyoplankton assemblages of the three regions in the northeast Pacific Ocean described here form the basis for future investigations of spatial and temporal patterns in the ichthyoplankton of the subarctic Pacific.Regional Index Terms: Northeast Pacific Ocean, Southeast Bering Sea, Gulf of Alaska, U.S. West Coast.     

厄尔尼诺现象和浙江近海鲐鲹鱼渔获量变化关系──长江口ENSO渔场学问题之二

本文研究浙江近海1970年到1992年鲐鱼渔获量变化与Elnino现象的关系，结合捕捞强度，长江中下游汛期（4—9月）降水量距平年际变化综合分析，探讨浙江近海鲐鱼渔获量变动规律，为合理开发渔业资源提供科学依据。