Methods for extraction of microorganism DNA from glacier surface snow

In order to thoroughly investigate the diversity of glacier microorganisms, four DNA extraction methods with different lysis patterns were tested and two screened methods (the Bosshard-Bano method and the Zhou method) were optimized for the most effective form of the filter membrane (cut vs. uncut), the DNA extraction method, and the precipitation method. The two optimized methods were then compared with the commercial Mo-Bio DNA extraction kit, and the results showed that the kit was generally suitable for extraction of microorganism DNA from glacier surface snow. Procedurally, it was found that a modified Bosshard- Bano method (i.e., cutting the filter membrane into pieces, using a specific lysis pattern [lysozyme (5 mg/mL)-protease K (1 mg/mL)-CTAB (1%)-SDS (1%)], performing the extraction only once by chloroform-isoamyl alcohol (24:1), and conducting DNA precipitation by pure ethanol) was also an effective and less expensive method for extraction of microorganism DNA from glacier surface snow.     

Transnational technology transfer network in China: Spatial dynamics and its determinants

Patent transfer has been regarded as an important channel for the nations and regions to acquire external technology, and also a direct research object to depict the relationship between supply and demand of technology flow. Therefore, based on traceable patent transfer data, this article has established a dual-pipeline theoretical framework of transnational-domestic technology transfer from the interaction of the global and local(glocal)perspective, and combines social networks, GIS spatial ana...     

甘肃省“五化”协同发展时空演变格局及影响因素分析

以2005年、2010年和2015年甘肃省87个县区、县级市及自治县的工业化、城镇化、信息化、农业现代化与绿色化（简称“五化”）为研究对象,利用空间自相关分析、地理加权回归分析等空间分析方法,结合构建的“五化”协同发展模型,对甘肃省“五化”协同发展的时空分异格局、时空演变趋势和影响因素进行研究分析,以期为甘肃省各市州科学的制定发展政策提供参考。结果表明：甘肃省“五化”协同发展格局具有明显的空间相关性且空间差异显著,协同水平呈现出明显的西北高、东南低分布格局;“五化”发展水平和综合水平较低,协同水平总体不高,以轻度失调、濒临失调和勉强协同为主,但总体上升幅度较大;“五化”协同发展的影响因素,按其影响力大小依次为：农村居民人均可支配收入 > 城乡居民可支配收入差 > 固定资产投资总额 > 财政支出 > 地形起伏度 > 城乡居民消费差 > 降水量。     

A new methodology for measuring coastline recession using buffering and non‐linear least squares estimation

Coastline recession is one of the best indicators of coastal erosion. Three methods for computing coastline recession – the baseline approach, the dynamic segmentation approach and the area‐based approach – have been used, each of which has one or more drawbacks. To overcome these problems, a new methodology for measuring coastline recession is proposed, using buffering and non‐linear least squares estimation. The proposed method was compared with the three existing methods with respect to two simulated cases and two real coastlines. Test results confirmed that the new method is more reliable than the three other methods, all of which are susceptible to variability of recession, scale, number of line segments, length of coastlines and direction of the baseline. The proposed method, incorporating two physically meaningful values – magnitude and variability of coastline recession according to the mean and standard deviation of coastline offsets, respectively – presents itself as an effective alternative method of assessing coastline recession.     

利用TM数据提取干旱区土地覆被信息的方法比较

以柴达木盆地香日德绿洲作为研究实验区,对该区域ETM遥感数据经过空间分辨率融合、主成分分析等方法进行空间信息增强及专题信息增强处理,组合最佳视觉背景图像,分别在不同背景图像上选择训练样本,利用最大似然法监督分类方法(MLC)、多空间尺度分层聚类(SSHC)和基于知识的模糊聚类方法(KFC)等分类器,分别用各自训练样本初始化各类别信息特征值,形成类别特征值模式库,分别以此为基础对待分样本进行分类,对初分类的结果经过类别合并、碎斑滤除以及重新编码赋色等分类后处理,得到最终分类结果及分类精度评价结果。从所获数据可以得出如下结论：从总体精度和Kappa值可知,SSHC和．KFC分类方法所获结果精度较高,总体精度比MLC分类结果约高于3％,SSHC之结果精度略高于KFC之结果;SSHC、KFC和MLC三种分类方法对该区域地表覆被信息的提取分类中,SSHC分类方法对耕地、石砾地、河滩和荒漠分类结果较好,KFC分类方法对耕地、沙地、河滩和荒漠分类结果较好,MLC分类方法对耕地、河滩和荒漠分类结果较好,三种分类方法对耕地、河滩和荒漠等三种地类的分类精度较高,用户精度都在80％以上,而对沙地和石砾地的分类结果其用户精度大都低于80％。     

基于熵值法的淮海经济区城市中心性评价

根据城市中心性的内涵,从生产中心性、商贸中心性、信息中心性、交通中心性和科教中心性5方面构建城市中心性的综合评价指标体系,并运用熵值法实证分析了淮海经济区主要城市的中心性.淮海经济区城市的中心性主要表现为生产、信息和科教方面的中心性,且区域差异较小,该区主要城市的中心性均不太显著,其中徐州的城市中心性相对较强.     

肝脏肺吸虫病的CT诊断

作者认为肺吸虫病发生在肝脏系少见病例，以ＣＴ认识和诊断上有待进一步提高，特别与肝结核、肝脓肿、肝包虫囊肿等鉴别诊断，仍存在一定的困难。临床上肺吸虫病多见脑部，肺，及皮下组织，我院病理科近十余年在肝脏发现肺吸虫病也是仅有一例，上述疾病ＣＴ表现均可出现低密度坏死区，有规则环状增强，斑点状钙化灶，单房及改变及大小不等的结节。组织学上的改变，肝细胞混浊肿胀，液化，变性，坏死，钙化，所不同处寄生虫病的病理组     

松潘地区岩金统计成矿予测中泛克立格法的应用

川西北地区是国内重要的岩金普查靶区。本文对松潘地区的地质特征,泛克立格法原理,及在本区的应用情况作了介绍,对主要计算步骤,给出基本结果,对计算获得的有意义异常区,进行地质解释,并将此方法与趋势面分析进行对比。     

基于三角模糊数随机模拟的地下水环境系统综合风险评价模型

采用随机模拟方法模拟三角模糊数,把三角模糊数及函数间运算简化为普通的实数之间运算,建立基于三角模糊数随机模拟的地下水环境系统综合风险评价模型(ARA-SSTFN)。结果说明:以置信区间形式表示的ARA-SSTFN评价结果,比现有常规方法结果提供评价结果可靠性方面更多信息,能反映受多种不确定性因素综合影响的地下水环境系统综合风险评价客观实际情况;ARA-SSTFN在流域水资源、水环境和水旱灾害等有随机性、模糊性和数据资料不精确等多种不确定性因素综合作用的各种资源环境系统综合风险评价问题中有推广应用价值。     

黄土区人类活动影响下的 产汇流模拟研究

日益频繁的人类活动改变了流域下垫面条件,对流域产汇流产生很大的影响。本文以黄河中游典型支流岔巴沟为研究区域,提出利用基于DEM的分布式水文模拟技术,探讨流域人类活动过程中的产汇流模拟,避免了经验公式的概化和由此引起的局限。模拟的结果证实了该方法的可行性。采用网格滞蓄的方法可以在子网格上体现人类活动引起的下垫面的变化及其对产汇流的影响,反映各个时期的产汇流条件,对降雨做出合理响应。