喻意 小人物反映大剧作 咏情 小婢女牵出大剧情 示理 小夫人折射大剧意 运城 以关汉卿的名著《调风月》使其地名增加了无限的光彩

《调风月》,是元代伟大的剧作家关汉卿的作品,全名《诈妮子调风月》,有《元刻古今杂剧三十种》本、《元人杂剧全集》本、《世界文库》本。《调风月》是元杂剧中,婢女形象塑造得最鲜明的代表作之一。这个剧对于我们认识当时黑暗的社会,了解下层妇女的不幸命运和统治阶级的虚伪、凶残有一定的意义。     

溶藻弧菌acfA基因克隆与生物信息学分析

溶藻弧菌引起海水养殖产业弧菌病的主要病原,附着定植因子基因acfA是溶藻弧菌重要毒力基因之一,其表达产物是溶藻弧菌有效定植在宿主肠道上所必需的蛋白。根据弧菌属细菌acfA基因基因保守区设计引物,采用Touchdown PCR扩增acfA基因部分序列,Inverse PCR和Nested PCR扩增已知序列侧翼序列,成功克隆了溶藻弧菌acfA基因全长。克隆的acfA基因序列全长为743 bp,开放阅读框长度为648 bp组成,共编码215个氨基酸,在5′端上游未发现-35区和-10区序列。演绎的ACFA蛋白N端有18个氨基酸信号肽序列,表明该蛋白是分泌型蛋白;蛋白结构分析表明主要由α螺旋和不规则卷曲构成。BLAST分析表明,该蛋白氨基酸序列与其他弧菌相应蛋白同源性较高,是较保守的外膜蛋白。     

Molecular Phylogeny ofParapenaeopsis Alcock, 1901（Decapoda： Penaeidae） Based on Chinese Materialsand 16S rDNA and COI Sequence

The phylogenetic relationship among Chinese Parapenaeopsis species was studied by comparing 16S rDNA and CO1gene sequence. The results showed that Parapenaeopsis hardwickii （Miers, 1878） and P cultrirostris Alcock, 1906 identified fromChinese samples were the same species. Parapenaeopsis cornuta （Kishinouye, 1900）, P. incisa Liu and Wang, 1987, and P. sinicaLiu and Wang, 1987 were different species. However, their systematic relationship were closer than those of other species, and theyformed an advanced branch in the phylogenetic trees. Additionally, the systematic position of P. hardwickii （Miers, 1878）, P. hun-gerfordi Alcock, 1905, and P. tenella （Bate, 1888） were not stable in the phylogenetic trees. More genetic markers such as 18S rDNAand 28S rDNA were supposed to be used to confirm the phylogenic relationship of Parapenaeopsis species.     

青蛤(Cyclina sinensis)TLR2基因的克隆与表达分析

利用转录组文库分析得到青蛤(Cyclina sinensis)的Toll样受体2(Toll-like receptor 2,TLR2)基因序列,采用荧光定量PCR法分析该基因的表达过程。结果显示,青蛤TLR2基因的cDNA开放阅读框为2082bp,编码693个氨基酸,具典型的LRRs、单次跨膜区和TIR结构域。该基因在血淋巴中的表达量最高,与肝脏、鳃、外套膜、闭壳肌和性腺有显著性差异(P0.05)。在鳗弧菌和Poly I:C刺激下,青蛤血淋巴中TLR2基因3h开始升高,48h达到最大值,与对照组和空白组有极显著性差异(P0.01);藤黄微球菌刺激下,血淋巴中CsTLR2基因3h开始升高,48h亦达到最大值,实验组与对照组有显著性差异(P0.05)。     

三疣梭子蟹(Portunus trituberculatus)胞内氯离子通道蛋白基因克隆及其表达分析

本研究克隆了三疣梭子蟹(Portunus trituberculatus)胞内氯离子通道蛋白基因,命名为Pt CLIC。该基因c DNA全长2000bp,5’和3’非编码区(UTR)长分别为76bp和1162bp,开放阅读框长762bp,推测编码253个氨基酸,预测分子量为29.2k Da,理论等电点为5.93。生物信息学分析表明,Pt CLIC基因中未发现跨膜结构域,属于不稳定蛋白;同源性分析表明,Pt CLIC基因编码的氨基酸序列与蚤状溞(Daphnia pulex)CLIC基因的同源性高达83%;系统进化分析表明,三疣梭子蟹与拟穴青蟹首先聚为一支;实时荧光定量RT-PCR分析表明,Pt CLIC基因在选取的所有组织中均有表达,在肝胰腺中的相对表达量最高,且显著高于其它组织(P0.05)。低盐度胁迫显著改变了Pt CLIC基因在三疣梭子蟹鳃和肝胰腺中的表达模式,整体呈先上调后下调的趋势,并发现该基因在低盐耐受和低盐敏感家系中的表达规律差异显著。研究结果表明Pt CLIC基因在三疣梭子蟹渗透压调节中发挥重要作用,可辅助三疣梭子蟹耐低盐品系的选育。     

Cloning and expression analysis of the chloroplast fructose- 1,6-bisphosphatase gene from Pyropia haitanensis

Fructose-1,6-bisphosphatase(FBPase) is one of the key enzymes in Calvin circle and starch biosynthesis. In this study, the full-length of cpFBPase gene from Pyropia haitanensis was cloned by using rapid amplification of cDNA ends(RACE) technology. The nucleotide sequence of PhcpFBPase consists of 1 400 bp, including a 5′ untranslated region(UTR) of 92 bp, a 3′?UTR of 69 bp, and an open reading frame(ORF) of 1 236 bp, which can be translated into a 412-amino-acid putative peptides with a molecular weight of 44.3 kDa and a theoretical pI of 5.23. Multiple sequence alignment indicated that the protein belonged to the chloroplast FBPase enzyme. Phylogenetic analysis showed that the protein assembled with the cpFBPase of a thermal tolerant unicellular red micro-algae Galdieria sulphuraria. Expression patterns analyzed by qRT-PCR revealed that the expression of PhcpFBPase gene in the thallus phage was 7-fold higher than in the conchocelis phage, which suggested the different mechanisms of inorganic carbon utilization among the different life phages of P. haitanensis. And the different response modes of PhcpFBPase mRNA levels to high temperature and desiccation stress indicated that PhcpFBPase played an important role in responsing to abiotic stress.     

牙鲆NPY的体外表达及体内检测

神经肽Y(Neuropeptide Y,NPY)被普遍认为是一种重要的促食因子,在调节鱼类的摄食行为方面起着至关重要的作用。为进一步研究牙鲆NPY蛋白的生物学功能,作者克隆了牙鲆NPY成熟肽序列(m-NPY)及含有信号肽的全长序列(f-NPY),利用原核表达系统分别进行体外重组表达,筛选出诱导剂IPTG最佳诱导浓度为0.8 mmol/L及最佳诱导时间3 h;此外,根据牙鲆NPY第49-64位氨基酸序列制备了多克隆抗体并通过Western blot验证该多抗能够有效检验重组NPY及牙鲆体内NPY的表达。研究结果为研究重组牙鲆NPY蛋白在牙鲆水产养殖产业中的应用及检测提供了依据。     

Cloning and Characterization of Glyceraldehyde-3-phosphate Dehydrogenase Encoding Gene in Gracilaria/Gracilariopsis lemaneiformis

Glyceraldehyde-3-phosphate dehydrogenase （GAPDH） plays important roles in various cellular processes. A cytosolic GAPDH encoding gene （gpd） of Gracilaria/Gracilariopsis lemaneiformis was cloned and characterized. Deduced amino acid sequence of the enzyme of G. lemaneiformis had high homology with those of seven red algae. The 5＇-untranslated regions of the GAPDHs encoding genes of these red algae varied greatly. GAPDHs of these red algae shared the highly conserved glyceraldehyde 3-phosphate dehydrogenase active site ASCTTNCL. However, such active site of Cyanidium caldarium was different from those of the other six algae at the last two residues （CL to LF）, thus the spatial structure of its GAPDH active center may be different from those of the other six. Phylogenetic analysis indicated that GAPDH of G. lemaneiformis might have undergone an evolution similar to those of Porphyra yezoensis, Chondrus crispus, and Gracilaria verrucosa. C. caldarium had a closer evolutionary relationship with Cyanidioschyzon merolae than with Cyanidium sp. Virtual Northern blot analysis revealed that gpd of G. lemaneiformis expressed constitutively, which suggested that it might be house-keeping and could be adapted as an inner control in gene expression analysis of G. lemaneiformis.     

基于遗传克隆选择算法优化BP神经网络的地理信息预测研究

针对BP神经网络训练过程中的训练时间较长、完全不能训练或容易陷入局部极小值等问题,提出基于遗传克隆选择算法(CLOGA)优化BP神经网络的流程,克服BP算法的一些缺陷。并通过湖北省人口预测问题进行效果检验,得到满意的结果。     

元胞自动机和克隆选择算法的蓝藻水华预测模型

针对蓝藻水华频发影响水体质量和生态环境的问题,该文以太湖为例,探讨了元胞自动机和克隆选择算法在蓝藻水华预测方面的可行性。使用遥感经验算法计算水体叶绿素a浓度;将叶绿素a浓度分布数据作为预测模型的输入数据,将基于平衡方程的预报模型与元胞自动机进行耦合,对蓝藻水华时空变化过程进行了动态模拟;在预测方面实现了蓝藻水华空间分布情况的可视化,并采用克隆选择算法优化了预测模型中的重要参数。研究结果表明:利用克隆选择算法优化的元胞自动机模型能够在蓝藻水华预测中直观地显示蓝藻水华的时空变化特征;能够在短时间内重复性地预演蓝藻水华的变化过程,为决策者提供有用的参考。