Antiangiogenic activity of low-temperature lysozyme from a marine bacterium <Emphasis Type="Italic">in vivo</Emphasis> and <Emphasis Type="Italic">in vitro</Emphasis>

We extracted marine low-temperature lysozyme (MLTL), a novel lysozyme, from a marine microorganism through fermentation. Our previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity through antiangiogenesis. In this study, we extracted a high weight (39 kDa) and investigated its antiangiogenic activity in vivo and in vitro. Using zebrafish embryos as an in vivo study model, we found that treatment with MLTL significantly inhibited the growth of subintestinal vessels (SIVs) in a dose-dependent manner and that 400 μg/ml MLTL was sufficient to block the growth of SIVs. An in vitro study conducted using human umbilical vein endothelial cells (HUVECs) revealed that MLTL suppressed the proliferation, migration and tube formation of HUVECs in a dose-dependent manner. Interestingly, assays by flow cytometry and DNA electrophoresis indicated that MLTL was able to induce apoptosis of HUVECs. Moreover, further study demonstrated that the disruption of intracellular Ca²⁺ homeostasis may play an important role in MLTL induced apoptosis of HUVECs. Taken together, the results of this study demonstrate for the first time that MLTL inhibits angiogenesis through its pleiotropic effects on vascular endothelial cells and induces apoptosis through regulation of cellular Ca²⁺ levels. The results of this study also revealed a possible mechanism underlying the antiangiogenic effect of MLTL and suggested that MLTL may be a promising new antiangiogenic agent for use in cancer therapy.     

Marine bacterium strain screening and pyrethroid insecticide-degrading efficiency analysis

A pyrethroid insecticide-degrading bacterium, strain HS-24, was isolated from an offshore seawater environment. The strain, which can degrade cypermethrin(CYP) and deltamethrin(DEL), was identified as Methylophaga sp. The optimal culture and degradation conditions for CYP and DEL by strain HS-24 is pH 7 at 28°C. Under optimum culture conditions, strain HS-24 exhibited a broad degradation concentration range of 100, 200, 400, 600, and 800 mg/L for CYP and DEL. The metabolic intermediates were analyzed by NMR, which provided strong evidence that CYP and DEL removal occurred mainly because of a biological process. The toxicity of the degradation products of strain HS-24 was studied simultaneously by measuring the light output of the luminescence bacterium. This demonstrated that the biodegradation ability of strain HS-24 significantly decreased the toxicity of CYP- and DEL-contaminated aquaculture seawater. Finally, the findings of this paper indicate that strain HS-24 is thus revealed as a biological agent for the remediation of marine aquatic environments.     

行播冬小麦热辐射方向性的影响因子

混合像元的热辐射方向性由于受到多种因素的影响而变得极为复杂。该文以行播冬小麦为例，建立矩阵模型，研究混合像元热辐射方向性问题。结果表明，混合像元热辐射方向性主要受到混合像元内部各组分在三维上分布的不均一、像元比辐射率的方向性以及由外界影响造成的各组分温度差异的影响。在这三个主要影响因子中，各组分的比辐射率是地物固有的特征，不随外界条件而发生变化。在同温条件下，像元热辐射方向性完全由像元比辐射率的方向性决定。而各组分温度虽然没有方向性，但可以通过组分空间分布对其产生调制作用。     

用比电导法研究活性炭的吸附特性

用比电导法研究两种活性炭自稀水溶液中吸附强电解质硫酸铬和硫酸铜的吸附平衡特性。结果表明,在本文的研究条件下,两种活性炭对硫酸铬和硫酸铜都有吸附作用。同时,活性炭吸附硫酸铜的吸附平衡特性可以用 Freundlich 方程式来描述。研究的结果对固体在溶液中的吸附基础理论以及处理工业废水具有一定的意义。     

BS活性土壤生态

公司简介;技术原理;技术特点;施工流程     

绝对重力仪激光器光纤对准调节方法概述

绝对重力仪激光器光纤由于应力变化、形状改变、震动或耦合器维护等原因破坏了输出激光的偏振态,使其在光纤内的传输变得非常不稳定,导致光纤输出激光的消光比值小于100,无法满足绝对重力观测条件。针对该问题,介绍了光纤对准调节的方法,通过设计消光比检测装置检验消光比值是否大于100。对#181和#250两台WEO100型He-Ne激光器的光纤进行对准调节实验,并通过消光比检测装置实测,两台激光器输出的光束消光比值满足绝对重力测量的条件,表明该对准调节方法科学有效。     

冻藏龙眼果皮细胞膜透性、PPO活性和酚类物质含量的变化

将“油谭本”龙眼在-40℃条件下进行速冻，然后转入-18℃恒定低温冷库中冻藏180d。测定了龙眼果实速冻时的冻结曲线、冻藏过程中果皮细胞膜渗透率、果皮内PPO的活性和总酚含量的变化情况。试验结果表明，-40℃的冷冻温度即可满足速冻要求；冻藏可以有效地抑制果皮内PPO的活性，果皮细胞膜渗透率和总酚含量的变化较小，能够较好地保持果实的外观品质。     

粉喷桩在软基处理中的应用研究

分析了粉喷桩加固软土地基的工作原理,总结了在软基处理中的应用经验,对粉喷桩的设计提出了见解。     

鄱阳湖水域和洲滩围垦后土壤的真菌多样性

于2011年5月,以鄱阳湖围垦38 a的水域和洲滩为研究区,采集水稻田、菜地、鱼塘和荒地的0~20 cm深度的土壤样品,对其理化因子进行测定,采用变性梯度凝胶电泳(DGGE)方法,对其真菌群落结构进行分析;运用典范对应分析,研究不同土地利用方式下表层土壤真菌群落的主要影响因子。结果表明,各土地利用方式下,土壤容重、含水量、p H、总有机碳含量、全氮含量、硝态氮含量和真菌生物量存在显著差异,鱼塘底泥中的真菌生物量最高,其次是荒地和菜地,水稻田土壤的真菌生物量最低;DGGE图谱结果显示,真菌群落结构也存在明显的差异,荒地与其他采样地差异明显,水稻田土壤和鱼塘底泥的真菌群落结构最为相似;相关性分析结果显示,除全磷含量以外,所测定的其他土壤理化因子和真菌生物量都显著影响真菌群落结构变化,其中起决定作用的理化因子为含水量、总有机碳含量和土壤容重。土地利用方式的改变造成土壤理化性质的改变,从而间接影响土壤真菌群落结构与活性。     

Labile organic matter content and distribution as affected by six-year soil amendments to eroded Chinese mollisols

Labile organic carbon （LOC） is a fraction of soil organic carbon （SOC） with rapid turnover time and is affected by soil fertilization. This investigation characterized the SOC content, LOC content and LOC distribution in the treatment plots of surface soil erosion at five levels （0-, 5-, 10-, 20- and 30-cm erosion）. The soil had received contrasting fertilizer treatments （i.e., chemical fertilizer or chemical fertilizer ＋ manure） for 6 years. This study demonstrated that both SOC and various LOC fractions contents were higher in the plots with fertilizer ＋ manure than in those with fertilizer alone under the same erosion conditions. The SOC and LOC contents de- creased as the erosion depth increased. Light fraction organic carbon, particulate organic carbon, easily oxidizable organic carbon （KMnO4-oxydizable organic carbon）, and microbial biomass carbon were 27% 57%, 37%-7%, 20%-25%, and 29%-33% higher respectively in the fertilizer ＋ manure plots, than in the fertilizer alone plots. Positive correlations （p 〈 0.05） between SOC content and different fractions contents were observed in all plots except the correlation between total SOC content and water-soluble organic carbon content in the different fertilization treatments. Obviously, fertilizer ＋ manure treatments would be conducive to the accumulation of LOC and SOC in the Black soil of Northeast China.