基于PPGIS的乡村旅游社区景观价值变化研究——以丽江束河古镇为例

景观价值是人对环境的主观感知和效用评价,景观价值变化的研究能够帮助理解旅游社区的社会现象.以丽江束河古镇为例,采用参与式制图与半结构化访谈方法调查当地居民对旅游开发前后的感知景观价值,最终获取的有效问卷与填图为293份,提取空间数据点658个.通过对访谈结果进行编码和归纳得到旅游开发前后各7种景观价值类型.采用质性分析...     

居民感知视角下遗产地旅游空间生产研究——以江苏省无锡市南长街为例

运用定量和质性研究方法,以大运河文化遗产无锡市南长街为例,对居民感知视角下的旅游空间生产进行探究。结果表明：（1）在与居民身份重构的关联分析中,居民对旅游影响下的社会空间、生态空间、文化空间3种类型空间演化感知影响度呈现逐渐递减的状态。（2）旅游空间生产下社区性的转变对居民主体产生了明显的“挤出与吸纳”效应,使得南长街居民类型更加复杂化。（3）旅游发展过程中居民对旅游空间生产存在被动性的心理审视视角,同时对南长街旅游空间生产存在“我者、他者”并存的感知心理。（4）对南长街居民在旅游空间生产过程中“游离或融入”的心理定位分析发现,居民在旅游空间生产过程中对社会空间演化存在感知度、融入性均较高的表达状态,而对生态空间与文化空间的旅游实践则持以游离性的旁观者角色。     

缀锦蛤亚科(Tapetinae)贝类线粒体DNA序列的系统学分析

采用线粒体16S rRNA和COI序列扩增和测序方法,对隶属于缀锦蛤亚科5属7种动物进行了系统学分析.经Clustal x多重比对和PAuP 4.10 软件分析,得到种间序列的遗传距离并构建了邻接(NJ)系统树.实验数据显示,缀锦蛤亚科为遗传连续的同源类群,其中的浅蛤属(Gomphina)、缀锦蛤属(Tapes)和蛤仔属(Ruditapes)亲缘关系较近,结果与Fischer-Piette等(1971)的缀锦蛤亚科的分类方案基本一致.另外,菲律宾蛤仔R.philippinarum 和杂色蛤仔 R.variegata是蛤仔属在印度洋和太平洋海区的一个组群,尽管两种贝类有明显的重叠分布区和相近的贝壳形态,但二者间的16S rRNA 和COI序列遗传距离均达到了物种间差别.结果支持庄启谦(2001)有关菲律宾蛤仔与杂色蛤仔的形态分类及分布区划分的观点.     

溶藻弧菌asp基因在大肠杆菌中表达活性研究及条件优化

为进一步研究溶藻弧菌碱性丝氨酸蛋白酶(ASP)蛋白生物学活性和功能,将构建的pET23d-asp在大肠杆菌BL21(DE3)中表达,对表达产物进行纯化分析,并优化表达条件。结果表明:在咪唑洗脱缓冲液浓度为100 mmol/L时,表达的可溶性ASP被大量洗脱,纯化的ASP相对分子质量约为42 000,具有蛋白活性;在诱导温度28℃、异丙基-β-D硫代半乳糖苷(Isopropyl-β-D-thiogalactopyranoside,IPTG)浓度1 mmol/L及诱导时间16 h时,表达的活性ASP蛋白最多。     

对虾传染性皮下及造血组织坏死病毒衣壳蛋白基因的克隆表达及抗体制备

本文根据GenBank登录的对虾传染性皮下及造血组织坏死病毒(IHHNV)的全基因组序列(NC-002190),设计出主要结构蛋白基因的相应引物,从厦门发病的对虾中,提取DNA,以此为模板,利用PCR扩增目的基因.再将目的基因分别与pGEX-6p1载体和pET-28a载体连接,构建重组表达载体pGEX-Ⅳ、pET-Ⅳ,重组子经酶切和测序鉴定后导入表达型大肠杆菌BL21,IPTG诱导表达,SDS-PAGE检测,大小约为62KDa和41KDa,与预测大小一致.将pET-Ⅳ表达重组蛋白经纯化,免疫小鼠,Western免疫印迹反应结果表明其抗体均可与pGEX-Ⅳ和pET-Ⅳ表达的蛋白发生特异性反应.本研究的结果为对虾IHHNV的快速检测及其免疫提供了研究基础.     

连云港连岛海滨旅游度假区三维地形景观图制作

本文以连云港市连岛为例,利用1∶250 00地形图在Arcview GIS中建立DEM,采用ERDAS IMAGINE良好的三维显示功能,在自定义的投影坐标系统下,通过DEM和遥感正射影像的叠加,生成三维地形景观图,同时阐述了三维地形景观图是一种由基础测绘数字产品相互组合而形成的一种新型的复合测绘产品,并展望了三维地形景观图的应用前景.     

牙鲆GHR基因Promoter区微卫星序列多态性与生长性状关系的初步研究

采用牙鲆GHR基因5'端Promoter区的1个微卫星标记,对胶南和日照2个牙鲆养殖群体进行了群体遗传多样性的研究,并探索该基因多态性位点与牙鲆生长性状之间的相关性.结果表明,2个群体在该座位的等位基因数为12和9个,有效等位基因数为6.26和5.04个,多态信息含量为0.84和0.80.2个群体该座位的Hardy-Weinberg遗传偏离指数均为正值,并没有显示出杂合子缺失,但各基因型分布频率都在一定程度上偏离Hardy-Weinberg平衡(P<0.01).连锁分析中发现,在胶南群体中,IM基因型对应的个体在全重、全长、体长、头长、体高和眼径形态学数据中均是最大的;在日照群体中,BC基因型对应的个体在全重、全长、体高、尾柄高、尾柄长和眼径数据中均是最大的;而CJ基因型对应的个体在体长和头长这两组数据中是最大的.该结果为研究GHR基因的变异对鱼类生长发育的影响及探索将该基因作为生长遗传标记的可行性奠定了实验基础.     

Landscape Dynamics and Driving Factors in Da＇an County of Jilin Province in Northeast China During 1956-2000

The landscape pattern of Da＇an County, Northeast China has undergone significant changes since the 1950s as a result of climatic change and human activities. The aim of this paper is to quantitatively study landscape pattern and its spatial dynamics of Da＇an County at the landscape level over the nearly 50-year span. Patch dynamics were examined according to land use and land cover change processes built from a series of images, as well as topographic maps, and temporal patterns built from landscape pattern metrics. The transition matrix of landscape patch types and changes of various landscape metrics were applied. The results showed that, from 1956 to 2000, the landscape within the study area had undertaken a complicated evolution in landscape structure and composition. The outstanding characteristic is that saline-alkali land increased and grassland decreased. As some smaller patches amalgamated, the heterogeneity of patch decreased. All those changes were the synthetic result of both climatic and anthropogenic influences, but the predominant factor was different in different parts. In the southern part of the study area, the landscape pattern changes resulted from the modification of climate obviously, while in the northern part, the landscape pattern changes were mainly caused by human activities, such as the conversion between farmland and saline-alkali land. This phenomenon showed that human activities played more important role in the north than in the south of Da＇an County.     

Landscape Effects of Land Consolidation Projects in Central China——A Case Study of Tianmen City,Hubei Province

The goal of land consolidation in China is still to develop agricultural production. The study of landscape effects of land consolidation projects （LCPs） faces many difficulties because of the lack of government＇s interest and data. This paper, taking Tianmen City of Hubei Province in Central China as an example, presents a methodology for analyzing landscape effects of LCPs by GIS and Fragstats3.3. It describes landscape effects with indexes of Patch Density （PD）, Largest Patch Index （LPI）, Landscape Shape Index （LSI）, Interspersion and Juxtaposition Index （IJI）, Aggregation Index （AI）, and Shannon＇s Diversity Index （SHDI）, showing more regular shape, simpler structure and less habitat diversity after LCPs. It computes ten landscape indexes of four categories of patches including Cultivated Land, Road, Water Channel, and River and Pond. The indexes show that 1） cultivated land becomes more fragmental in patch area, less irregular in patch shape and more concentrated in block; 2） the transport capacity of roads and irrigation and drainage capacity of water channels have been improved; 3） the landscape change of river and pond can be summarized as decreasing scale, more regular shape, reducing connectivity and diversity of the class. LCPs can facilitate agricultural production as well as protect cultivated land and food security. However, it is doubted that the increase of cultivated land from LCPs results from the reducing in landscape diversity of water area.